[논문]Comparison of Acid and Bile Tolerances, Cholesterol Assimilation, and CLA Production in Probiotic Lactobacillus acidophilus Strains

Oh, Se-Jong; Chai, Chang-Hun; Kim, Sae-Hun; Kim, Young-Jun; Kim, Hyung-S.; Worobo, Randy W.

doi:10.5851/kosfa.2012.32.4.409

Comparison of Acid and Bile Tolerances, Cholesterol Assimilation, and CLA Production in Probiotic Lactobacillus acidophilus Strains 원문보기

Korean journal for food science of animal resources = 한국축산식품학회지, v.32 no.4, 2012년, pp.409 - 413

Oh, Se-Jong (Division of Animal Science, Chonnam National University) , Chai, Chang-Hun (Divison of Food Science & Technology, Korea University) , Kim, Sae-Hun (Divison of Food Science & Technology, Korea University) , Kim, Young-Jun (Department Food & Biotechnology, Korea University) , Kim, Hyung-S. (Culture Systems Inc.) , Worobo, Randy W. (Department of Food Science & Technology, Cornell University)

Abstract ▼ AI-Helper

This study aimed to compare the probiotic characteristics of twelve strains of Lactobacillus acidophilus including cholesterol assimilation and conjugated linoleic acid (CLA) production. Cholesterol assimilation exhibited some variation among L. acidophilus strains, which could be classified into three groups based on their assimilation levels (p<0.05). The high cholesterol assimilation group exhibited a significantly higher tolerance to 0.3 and 0.5% bile acid than the low cholesterol assimilation group (p<0.05). Cholesterol assimilation showed positive correlation with 0.5% bile tolerance, and a negative correlation with acid tolerance (p<0.01). Glycocholate deconjugation activity showed no relationship with cholesterol assimilation, whereas taurocholate deconjugation activity was shown to have negative correlation with cholesterol assimilation (p<0.05). CLA production by L. acidophilus strains exhibited a wide variation, ranging from 2.69 to 5.04 mg/g fat. CLA production of L. acidophilus GP1B was the highest among the tested strains, but there was no evidence for differences in CLA production in strain specificity. Based on these results, the cholesterol assimilation of L. acidophilus strains may not be related to deconjugation activity, but may in-fact be attributed to their bile-tolerance.

주제어

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제안 방법

The purpose of this study was to determine cholesterol assimilation ability, acid and bile tolerances, deconjugation of glycocholate and taurocholate, and CLA production of probiotic strains of L. acidophilus.

데이터처리

All experiments were replicated six times and viable cell counts were performed in duplicate. Statistical analysis was performed on data using GLM and CORR procedures with SAS systems (SAS, 2008), to compare within groups and to establish if any relationship existed between the different characteristics.

이론/모형

The amount of cholesterol in the cell-free spent broth was determined by the method of Rudel and Morris (1973). A 0.

성능/효과

The high cholesterol assimilation group showed a higher tolerance to 0.3% and 0.5% oxgall than either the medium or low assimilation groups (p<0.05), whereas the low cholesterol assimilation group showed a higher acid tolerance than the high and medium assimilation groups (p<0.05).
The strains 4356, 30SC, 393, and 4962 were classified as the low assimilation group, within which cholesterol assimilation ranged from 28% to 36%. Strains 107A and GP4A, the medium assimilation group, showed 48.

후속연구

The possibility of strain variation was not completely ruled out, but the level of cholesterol assimilation appears to have a correlation with bile resistance. Further research is required in order to fully understand the mechanism of cholesterol assimilation in L. acidophilus strains.

참고문헌 (20)

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