Objectives : Diabetic nephropathy is associated with morbidity and mortality of diabetes mellitus patients. Mesangial cell proliferation is known as the major pathologic features such as glomerulosclerosis. Oryeong-san, Korean formula, is widely used for the treatment of nephrosis, edema, and uremia...
Objectives : Diabetic nephropathy is associated with morbidity and mortality of diabetes mellitus patients. Mesangial cell proliferation is known as the major pathologic features such as glomerulosclerosis. Oryeong-san, Korean formula, is widely used for the treatment of nephrosis, edema, and uremia. Oryeong-san is composed of five herbs: Alismatis Rhizoma, Polyporus, Atractylodis Rhizoma Alba, Hoelen, and Cinnamomi Cortex. Methods : The present study was performed to investigate potent inhibitory effect of Oryeong-san on high glucose (HG)-induced rat mesangial cells (RMC) proliferation. Results : RMC proliferation under 25 mM glucose was significantly accelerated compared with 5.5 mM glucose, which was inhibited by Oryeong-san in dose dependent manner. Pre-treatment of Oryeong-san induced down-regulation of cyclins/CDKs and up-regulation of CDK inhibitor, p21waf1/cip1 and p27kip1 expression. In addition, Oryeong-san reduced HG-induced RMC proliferation by suppressed the mitogen-activated protein kinase (MAPK) phospholyration such as extracellular signal regulated kinase (ERK), Jun N-terminal Kinase (JNK), and p38. Oryeong-san significantly suppressed HG-induced ROS production. Conclusions : Oryeong-san consequently inhibited HG-induced mesangial cell proliferation through the inhibition of MAPK and ROS signaling pathway. These results suggest that Oryeong-san may be effective in the treatment of renal dysfunction leading to diabetic nephropathy.
Objectives : Diabetic nephropathy is associated with morbidity and mortality of diabetes mellitus patients. Mesangial cell proliferation is known as the major pathologic features such as glomerulosclerosis. Oryeong-san, Korean formula, is widely used for the treatment of nephrosis, edema, and uremia. Oryeong-san is composed of five herbs: Alismatis Rhizoma, Polyporus, Atractylodis Rhizoma Alba, Hoelen, and Cinnamomi Cortex. Methods : The present study was performed to investigate potent inhibitory effect of Oryeong-san on high glucose (HG)-induced rat mesangial cells (RMC) proliferation. Results : RMC proliferation under 25 mM glucose was significantly accelerated compared with 5.5 mM glucose, which was inhibited by Oryeong-san in dose dependent manner. Pre-treatment of Oryeong-san induced down-regulation of cyclins/CDKs and up-regulation of CDK inhibitor, p21waf1/cip1 and p27kip1 expression. In addition, Oryeong-san reduced HG-induced RMC proliferation by suppressed the mitogen-activated protein kinase (MAPK) phospholyration such as extracellular signal regulated kinase (ERK), Jun N-terminal Kinase (JNK), and p38. Oryeong-san significantly suppressed HG-induced ROS production. Conclusions : Oryeong-san consequently inhibited HG-induced mesangial cell proliferation through the inhibition of MAPK and ROS signaling pathway. These results suggest that Oryeong-san may be effective in the treatment of renal dysfunction leading to diabetic nephropathy.
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제안 방법
In the experiment to determine the effect of high glucose (25 mM) on RMC proliferation, this study examined cell proliferation using MTT. Exposure of RMC to 25 mM D-glucose for 24 h induced cell proliferation (Fig.
RMC were incubated for 24 h with or without HG and Oryeong-san, and then cell viability and proliferation were assessed using a MTT assay. Results are expressed as the mean±S.
Therefore, the present study was performed to determine the possible effects of Oryeong-san decoction on the proliferation of primary RMC in response to high glucose (HG).
대상 데이터
CM-H2DCFDA was purchased from Invitrogen, Inc (Eugene, OR). Mouse monoclonal antibodies to p21waf1/cip1, p27kip1, JNK, ERK and p38 MAPK were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit polyclonal antibodies to CDKs, Cyclins, β-actin were purchased from Santa Cruz Biotechnology.
데이터처리
Unpaired Student's t test was used for comparison between two groups. For multiple comparisons, data were analyzed by one-way ANOVA with Post Hoc Multiple Comparisons. The independent experiments were performed at least 3 times with similar results.
성능/효과
. In conclusion, Oryeong-san has a protective effect on renal proliferation, suggesting that Oryeong-san is beneficial traditional decoction in the treatment of mesangial cell proliferation leading to diabetic nephropathy.
Some studies have shown a role for the CDKIs p21waf1/cip1 and p27kip1 in the proliferation of rat mesangial cell5-7). Our results showed that Oryeong-san inhibited cyclinD1/CDK4 and cyclinE/CDK2 activities by increasing the expression of p21waf1/cip1 and p27kip1 in rat mesangial cell following HG treatment. These results suggest that treatment with Oryeong-san induces a G0/G1 arrest in the cell cycle by increasing the expression of CDKIs, p21waf1/cip1 and p27kip1, and decreasing CDK2/4 activities.
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