잡곡류의 항혈전작용을 조사하기 위해 국내산 11종의 잡곡으로부터 80% 에탄올 추출물을 얻어 혈액응고저해활성을 thrombin time (TT)법으로 측정한 결과, 황금찰수수의 에탄올 추출물이 가장 높은 혈액응고저해활성을 보였으며, 뒤이어 청차조 유래의 에탄올 추출물도 혈액응고저해활성을 보였다. 황금찰수수의 에탄올 추출물을 n-hexane, methylene chloride, ethyl acetate 및 n-butanol로 분획하였을 때, 대부분의 TT-저해활성은 hexane과 methylene chloride 분획에 분포하였으며, 이들 분획의 활성은 동일 농도의 aspirin (최종 농도 480 ${\mu}g/ml$)보다 더 높은 것으로 나타났다. 또한 황금찰수수의 에탄올 추출물의 혈액응고저해활성은 activated partial thromboplastin time (APTT)법 및 prothrombin time (PT)법으로도 확인되었다. 이때 APTT-저해활성은 hexane 및 methylene chloride분획에 주로 분포하였으나 PT-저해활성은 hexane 분획에 주로 분포하였다. 이들 분획들의 APTT-저해활성 및 PT-저해활성은 warfarin (최종농도 3.13 mg/ml) 보다 더 높은 것으로 확인되었다. 황금찰수수 유래 에탄올 추출물의 TT-저해활성은 열 안정성 및 pH 안정성이 매우 우수하였다. 한편, 이와 같은 황금찰수수 유래 에탄올추출물, hexane 분획 및 methylene chloride 분획의 경우는 피브린 응괴를 가용화할 수 있는 피브린용해활성을 지닌 것으로 나타났다. 이러한 결과들은 황금찰수수가 혈액응고저해활성과 혈전용해활성을 지니고 있음을 보여주며, 아울러 항혈전 식이요법의 소재가 될 수 있음을 시사한다.
잡곡류의 항혈전작용을 조사하기 위해 국내산 11종의 잡곡으로부터 80% 에탄올 추출물을 얻어 혈액응고저해활성을 thrombin time (TT)법으로 측정한 결과, 황금찰수수의 에탄올 추출물이 가장 높은 혈액응고저해활성을 보였으며, 뒤이어 청차조 유래의 에탄올 추출물도 혈액응고저해활성을 보였다. 황금찰수수의 에탄올 추출물을 n-hexane, methylene chloride, ethyl acetate 및 n-butanol로 분획하였을 때, 대부분의 TT-저해활성은 hexane과 methylene chloride 분획에 분포하였으며, 이들 분획의 활성은 동일 농도의 aspirin (최종 농도 480 ${\mu}g/ml$)보다 더 높은 것으로 나타났다. 또한 황금찰수수의 에탄올 추출물의 혈액응고저해활성은 activated partial thromboplastin time (APTT)법 및 prothrombin time (PT)법으로도 확인되었다. 이때 APTT-저해활성은 hexane 및 methylene chloride분획에 주로 분포하였으나 PT-저해활성은 hexane 분획에 주로 분포하였다. 이들 분획들의 APTT-저해활성 및 PT-저해활성은 warfarin (최종농도 3.13 mg/ml) 보다 더 높은 것으로 확인되었다. 황금찰수수 유래 에탄올 추출물의 TT-저해활성은 열 안정성 및 pH 안정성이 매우 우수하였다. 한편, 이와 같은 황금찰수수 유래 에탄올추출물, hexane 분획 및 methylene chloride 분획의 경우는 피브린 응괴를 가용화할 수 있는 피브린용해활성을 지닌 것으로 나타났다. 이러한 결과들은 황금찰수수가 혈액응고저해활성과 혈전용해활성을 지니고 있음을 보여주며, 아울러 항혈전 식이요법의 소재가 될 수 있음을 시사한다.
To examine whether miscellaneous cereal grains have an antithrombotic effect, we investigated the anticoagulant activity of 80% ethanol extracts from eleven selected miscellaneous cereal grains. The 80% ethanol extract of hwanggeumchal sorghum (Sorghum bicolor) showed the highest anticoagulant activ...
To examine whether miscellaneous cereal grains have an antithrombotic effect, we investigated the anticoagulant activity of 80% ethanol extracts from eleven selected miscellaneous cereal grains. The 80% ethanol extract of hwanggeumchal sorghum (Sorghum bicolor) showed the highest anticoagulant activity, followed by that of green foxtail millet grains, in terms of thrombin time (TT). When the ethanol extract of hwanggeumchal sorghum was sequentially fractionated with n-hexane, methylene chloride, ethyl acetate, and n-butanol, the majority of the TT-inhibitory activity was detected in the hexane and methylene chloride fractions. Whereas aspirin (final conc. 480 ${\mu}g/ml$) prolonged TT by 2-fold, the ethanol extract, hexane fraction, and methylene chloride fraction in the same dose prolonged TT by 2.2-fold, 2.9-fold, and 2.5-fold, respectively. The ethanol extract of hwanggeumchal sorghum could delay activated partial thromboplastin time (APTT) as well as prothrombin time (PT). Although the APTT-inhibitory activity of the ethanol extract was mainly partitioned into the hexane and methylene chloride fractions, the PT-inhibitory activity of the ethanol extract was solely partitioned into the hexane fraction. The APTT- and PT-inhibitory activities of these organic solvent fractions were more potent than those of the control warfarin (final conc. 3.13 mg/ml). The TT-inhibitory activity of the ethanol extract was heat-stable and acid-stable. The ethanol extract, hexane fraction, and methylene chloride fraction of hwanggeumchal sorghum appeared to possess a direct fibrinolytic activity toward fibrin clotting. These results show that hwanggeumchal sorghum can exert anticoagulant and fibrinolytic effects and, thus, have the potential to be applicable as antithrombotic dietary sources.
To examine whether miscellaneous cereal grains have an antithrombotic effect, we investigated the anticoagulant activity of 80% ethanol extracts from eleven selected miscellaneous cereal grains. The 80% ethanol extract of hwanggeumchal sorghum (Sorghum bicolor) showed the highest anticoagulant activity, followed by that of green foxtail millet grains, in terms of thrombin time (TT). When the ethanol extract of hwanggeumchal sorghum was sequentially fractionated with n-hexane, methylene chloride, ethyl acetate, and n-butanol, the majority of the TT-inhibitory activity was detected in the hexane and methylene chloride fractions. Whereas aspirin (final conc. 480 ${\mu}g/ml$) prolonged TT by 2-fold, the ethanol extract, hexane fraction, and methylene chloride fraction in the same dose prolonged TT by 2.2-fold, 2.9-fold, and 2.5-fold, respectively. The ethanol extract of hwanggeumchal sorghum could delay activated partial thromboplastin time (APTT) as well as prothrombin time (PT). Although the APTT-inhibitory activity of the ethanol extract was mainly partitioned into the hexane and methylene chloride fractions, the PT-inhibitory activity of the ethanol extract was solely partitioned into the hexane fraction. The APTT- and PT-inhibitory activities of these organic solvent fractions were more potent than those of the control warfarin (final conc. 3.13 mg/ml). The TT-inhibitory activity of the ethanol extract was heat-stable and acid-stable. The ethanol extract, hexane fraction, and methylene chloride fraction of hwanggeumchal sorghum appeared to possess a direct fibrinolytic activity toward fibrin clotting. These results show that hwanggeumchal sorghum can exert anticoagulant and fibrinolytic effects and, thus, have the potential to be applicable as antithrombotic dietary sources.
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제안 방법
In order to screen eleven miscellaneous cereal grains for anticoagulant effect on thrombin-mediated coagulation, the 80% ethanol extracts from individual miscellaneous cereal grains were dissolved in DMSO, and then their effects on thrombin time (TT) were investigated. As shown in Fig.
To assess anticoagulant activity of sample, the effect of sample on thrombin time (TT) was determined using an Auto Blood Coagulation Analyzer (Sysmex CA-540, Japan), according to the manufacturer’s instructions.
To assess inhibitory effect of the sample on the intrinsic pathway of coagulation cascade, activated partial thromboplastin time (APTT) was determined using an Auto Blood Coagulation Analyzer (Sysmex CA-540), according to the manufacturer’s instructions.
대상 데이터
Eleven miscellaneous cereal grains, including proso millet (polished grains), yellow glutinous proso millet (unpolished grains), glutinous sorghum (polished grains), hwanggeumchal sorghum (unpolished grains), white glutinous sorghum (unpolished grains), yellow glutinous foxtail millet (polished grains), non-glutinous foxtail millet (polished grains), green glutinous foxtail millet (unpolished grains), golden foxtail millet (unpolished grains), barnyard millet (unpolished grains), and adlay (polished grains) harvested in Korea were provided by National Institute of Crop Science, Rural Development Administration, Miryang, Gyeongnam 627-803, Korea. As shown in Fig.
The thrombin time (TT)-inhibitory activity (%)=(coagulation time of sample/coagulation time of negative control)×100. The experiment was performed in triplicates.
데이터처리
The statistical significance was calculated with Student’s t-test.
성능/효과
In conclusion, current results show that the 80% ethanol extract from hwanggeumchal sorghum grains could exert not only anticoagulant activity in terms of TT, APTT and PT, but also fibrinolytic activity. These results suggest that hwanggeumchal sorghum grains might have a potential to be applicable as antithrombotic dietary source.
The results show that the 80% ethanol extract of hwanggeumchal sorghum grains possesses not only anticoagulant activity in terms of TT, APTT and PT, but also fibrinolytic activity.
0-fold, respectively, whereas aspirin at a concentration of 240 μg/ml was unable to prolong TT. These results confirm that the anticoagulant activity of 80% ethanol extract of hwanggeumchal sorghum grains is more potent than that of aspirin, and suggest that the anticoagulant activities of hexane fraction and methylene chloride fraction might be at least 3-fold stronger than that of aspirin.
These results showed that the 80% ethanol extracts obtained from hwanggeumchal sorghum grains and green foxtail millet grains exerted a remarkable anticoagulant effect among the eleven miscellaneous cereal grains tested. Current results also suggested that the anticoagulant activity of the 80% ethanol extract of hwanggeumchal sorghum grains, which was assessed by TT assay, was more potent than that of the positive control aspirin in the same dose (final conc.
참고문헌 (44)
Ajjan, R. and Grant, P. J. 2006. Coagulation and atherothrombotic disease. Atherosclerosis 186, 240-259.
Bazzano, L. A., He, J., Ogden, L. G., Loria, C. M., Vupputuri, S., Myers, L. and Whelton, P. K. 2002. Fruit and vegetable intake and risk of cardiovascular disease in US adults: the first national health and nutrition examination survey epidemiologic follow-up study. Am J Clin Nutr 76, 93-99.
Dahback, B. 2005. Blood coagulation and its regulation by anticoagulant pathways: Genetic pathogenesis of bleeding and thrombotic disease. J Intern Med 257, 209-223.
Fan, J., Zhang, Y., Chang, X., Zhang, B., Jiang, D., Saito, M. and Li, Z. 2009. Antithrombotic and fibrinolytic activities of methanolic extract of aged sorghum vinegar. J Agric Food Chem 57, 8683-8687.
Gillman, M. W., Cupples, L. A., Gagnon, D., Posner, B. M., Ellison, R. C., Castelli, W. P. and Wolf, P. A. 1995. Protective effect of fruits and vegetables on development of stroke in men. JAMA 273, 1113-1117.
Gwak, H. S. and Chun, I. K. 2000. Preformulation study of Aspalatone, a new antithrombotic agent. J Appl Pharm 8, 332-337.
Ha, Y. D. and Lee, S. P. 2001. Characteristics of proteins in Italian millet, sorghum and common millet. Korean J Postharvest Sci Technology 8, 187-192.
Hoffman, M. 2004. Remodeling the blood coagulation cascade. J Thromb Thrombolysis 16, 17-20.
Hoppe, B., Tolou, F., Dorner, T., Kiesewetter, H. and Salama, A. 2006. Gene polymorphisms implicated in influencing susceptibility to venous and arterial thromboembolism: frequency distribution in a healthy German population. Thromb Haemost 96, 465-470.
Jin, Y. R., Yu, J. Y., Lee, J. J., You, S. H., Chung, J. H., Noh, J. Y., Im, J. H., Han, X. H., Kim, T. J., Shin, K. S., Wee, J. J. and Yun, Y. P. 2007. Antithrombotic and antiplatelet activities of Korean red ginseng extract. Basic Clin Pharmacol Toxicol 100, 170-175.
Joshipura, K. J., Ascherio, A., Manson, J. E., Stampfer, M. J., Rimm, E. B., Speizer, F. E., Hennekens, C. H., Spiegelman, D. and Willett, W. C. 1999. Fruit and vegetable intake in relation to risk of ischemic stroke. JAMA 282, 1233-1239.
Joshipura, K. J., Hu, F. B., Manson, J. E., Stampfer, M. J., Rimm, E. B., Speizer, F. E., Colditz, G., Ascherio, A., Rosner, B., Spiegelman, D. and Willett, W. C. 2001. The effect of fruit and vegetable intake on risk for coronary heart disease. Ann Intern Med 134, 1106-1114.
Kil, H. Y., Seong, E. S., Ghimire, B. K., Chung, I. M., Kwon, S. S., Goh, E. J., Heo, K., Kim, M. J., Lim, J. D., Lee, D. K. and Yu, C. Y. 2009. Antioxidant and antimicrobial activities of crude sorghum extract. Food Chem 115, 1234-1239.
Kim, J. S., Hyun, T. K. and Kim, M. J. 2011. The inhibitory effects of ethanol extracts from sorghum, foxtail millet and proso millet on $\alpha$ -glucosidase and $\alpha$ -amylase activities. Food Chem 124, 1647-1651.
Kwak, C. S., Lim, S. J., Kim, S. A., Park, S. C. and Lee, M. S. 2004. Antioxidative and antimutagenic effects of Korean buckwheat, sorghum, millet and job's tears. J Korean Soc Food Sci Nutr 33, 921-929.
Liu, S., Manson, J. E., Lee, I. M, Cole, S. R., Hennekens, C. H., Willett, W. C. and Buring, J. E. 2000. Fruit and vegetable intake and risk of cardiovascular disease: the women' health study. Am J Clin Nutr 72, 922-928.
Marquart, L., Wiemer, K. L., Jones, J. M. and Jacob, B. 2003. Whole grain health claims in the USA and other efforts toincrease whole-grain consumption. Proc Nutr Soc 62, 151-160.
Melzig, M. F. and Henke, K. 2005. Inhibition of thrombin activity by selected natural products in comparison to neutrophil elastase. Planta Med 71, 787-789.
Nagda, K. K., Ganeriwal, S. K., Nagda, K. C. and Diwan, A. M. 1983. Effect of onion and garlic on blood coagulation and fibrinolysis in vitro. Indian J Physiol Pharmacol 27,141-145.
Park, M. Y., Kim, J. H. and Park, D. S. 2011. Anti-inflammatory activities of hog millet (Panicum miliaceum L.) in murine macrophages through IRAK-4 signaling. Korean J Food Nutr 24, 268-272.
Rajput, M. S., Mathur, V., Agrawal, P., Chandrawanshi, H. K. and Pilaniya, U. 2011. Fibrinolytic activity of kaempferol isolated from the fruits of Lagenaria siceraria (Molina) Standley. Nat Prod Res 25, 1870-1875.
Sahyoun, N. R., Jacques, P. F., Zhang, X. L., Juan, W. and McKeown, N. M. 2006. Whole-grain intake is inversely associated with the metabolic syndrome and mortality in older adults. Am J Clin Nutr 83, 124-131.
Slavin, J. L., Martini, M. C., Jacobs, D. R. and Marquart, L. 1999. Plausible mechanisms for the protectiveness of whole grains. Am J Clin Nutr 70, 459S-463S.
Sohn, H. Y., Kwon, C. S., Son, K. H., Kwon, G. S., Kwon, Y. S., Ryu, H. Y. and Kum E. J. 2005. Antithrombosis and antioxidant activity of methanol extract from different brands of rice. J Korean Soc Food Sci Nutr 34, 593-598.
Taussig, S. J. and Batkin, S. 1988. Bromelain. The enzyme complex of pineapple (Ananas comosus) and its clinical application. J Ethnopharmacol 2, 191-203.
Torres-Urrutia, C., Guzman, L., Schmeda-Hirschmann, G., Moore-Carrasco, R., Alarcon, M., Astudillo, L., Gutierrez, M., Carrasco, G., Yuri, J. A., Gwak, H. S. and Chun, I. K.2000. Antiplatelet, anticoagulant, and fibrinolytic activity in vitro of extracts from selected fruits and vegetables. Blood Coagul Fibrinolysis 22, 197-205.
Ulbricht, T. L. and Southgate, D. A. 1991. Coronary heart disease: seven dietary factors. Lancet 338, 985-992.
Wee, J. J., Kim, Y. S., Kyung, J. S., Song. Y. B., Do, J. H., Kim. D. C. and Lee, S. D. 2010.Identification of anticoagulant components in Korean red ginseng. J Ginseng Res 34, 355-362
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