Here, we present an approach of blood plasma proteome profiling and their comparisons between the young and the adult pigs as prerequisite for the identification of bio-markers related to the health conditions, growth performance and meat quality. To profile the proteome in porcine plasma, blood sam...
Here, we present an approach of blood plasma proteome profiling and their comparisons between the young and the adult pigs as prerequisite for the identification of bio-markers related to the health conditions, growth performance and meat quality. To profile the proteome in porcine plasma, blood samples were collected from 19 young piglets and 20 adult male barrows and the plasma was retrieved. Then, protein profiling was initiated using one and two-dimensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the results, more than thirty-six and twenty eight protein spots were selected in young piglets and adult pigs, respectively and twenty three proteins were identified. The proteome profile images were compared between those ones using Image Master Version 7.0. The image of expressed proteome showed that most of proteins from plasma of young piglet separated clearly and concentrated in 2DE display compared to ones from adult. Image analysis in detail was carried out to look for the specific proteins related to age progression. It demonstrated that the characteristics of proteome expression could be distinct to their age stages. Further investigations needed to proceed to understand the age dependent change of protein conformation and biological meaning of those differences in proteome expression between young and mature adult pigs.
Here, we present an approach of blood plasma proteome profiling and their comparisons between the young and the adult pigs as prerequisite for the identification of bio-markers related to the health conditions, growth performance and meat quality. To profile the proteome in porcine plasma, blood samples were collected from 19 young piglets and 20 adult male barrows and the plasma was retrieved. Then, protein profiling was initiated using one and two-dimensional electrophoresis. Proteins were spotted and then identified by MALDI-TOF-TOF and LC-MS-MS. In the results, more than thirty-six and twenty eight protein spots were selected in young piglets and adult pigs, respectively and twenty three proteins were identified. The proteome profile images were compared between those ones using Image Master Version 7.0. The image of expressed proteome showed that most of proteins from plasma of young piglet separated clearly and concentrated in 2DE display compared to ones from adult. Image analysis in detail was carried out to look for the specific proteins related to age progression. It demonstrated that the characteristics of proteome expression could be distinct to their age stages. Further investigations needed to proceed to understand the age dependent change of protein conformation and biological meaning of those differences in proteome expression between young and mature adult pigs.
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제안 방법
Data were collected in the “Triple Play” (MS scan, Zoom scan, and MS/MS scan) mode and filtered and analyzed by a proprietary algorithm.
대상 데이터
Blood samples were collected from 19 heads of crossbred 2 week old young male pigs weighing under 2 kg and 20 heads of 5 month old mature adult male barrow pigs weighing over 90 up to 120 kg which were managed at feeding barn in the National Institute of Animal Science. All experimental procedures and the care of animals were conducted in accordance with the guidelines of the animal Care and Use Committee (IACUC) of the National Institute of Animal Science in Korea.
이론/모형
The concentration of the total protein in the sample was determined by Bradford's protein assay method.
5% polyacrylamide sodium dodecyl sulphate gels (26×20 cm) with a constant voltage of 100 V for 30 min, 250 V for 6 hr using the EttanDALT II system (Amersham Bioscience, Piscataway, USA). The proteins were visualized using Coomassie Brilliant Blue (CBB) G-250 staining method.
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