Lee, Young Mee
(Genetics and Breeding Research Center, NFRDI)
,
Lee, Jeong-Ho
(Genetics and Breeding Research Center, NFRDI)
,
Noh, Jae Koo
(Genetics and Breeding Research Center, NFRDI)
,
Kim, Hyun Chul
(Genetics and Breeding Research Center, NFRDI)
,
Park, Choul-Ji
(Genetics and Breeding Research Center, NFRDI)
,
Park, Jong-Won
(Genetics and Breeding Research Center, NFRDI)
,
Hwang, In Joon
(Genetics and Breeding Research Center, NFRDI)
,
Kim, Sung Yeon
(Genetics and Breeding Research Center, NFRDI)
TCR subunits are members of membrane-bound receptors which allow the fast and efficient elimination of the specific fish pathogens have regulated function in adaptive immunity. Sequence structure of TCR subunits have been reported for various teleosts, but the information of each TCR subunit functio...
TCR subunits are members of membrane-bound receptors which allow the fast and efficient elimination of the specific fish pathogens have regulated function in adaptive immunity. Sequence structure of TCR subunits have been reported for various teleosts, but the information of each TCR subunit functional characterization through expression analysis in fish was unknown. In this study, we examined the gene expression of TCR subunits in the early developmental stages and observed transcript levels in various tissues from healthy adult olive flounder by RT-PCR. The mRNA expression of alpha subunit was already detected in the previous hatching step. But the transcripts of another TCR subunit were not observed during embryo development and increased after hatching and maintained until metamorphosis at the same level. It was found that all TCR subunits mRNAs are commonly expressed in the immune-related organ such as spleen, kidney and gill, also weak expressed in fin and eye. TCR alpha and beta subunit were expressed in brain, whereas gamma and delta were not expressed same tissue. The sequence alignment analysis shows that there are more than 80% sequence homology between TCR subunits. Because it has a high similarity of amino acid sequence to expect similar in function, but expression analysis show that will have may functional diversity due to different time and place of expression.
TCR subunits are members of membrane-bound receptors which allow the fast and efficient elimination of the specific fish pathogens have regulated function in adaptive immunity. Sequence structure of TCR subunits have been reported for various teleosts, but the information of each TCR subunit functional characterization through expression analysis in fish was unknown. In this study, we examined the gene expression of TCR subunits in the early developmental stages and observed transcript levels in various tissues from healthy adult olive flounder by RT-PCR. The mRNA expression of alpha subunit was already detected in the previous hatching step. But the transcripts of another TCR subunit were not observed during embryo development and increased after hatching and maintained until metamorphosis at the same level. It was found that all TCR subunits mRNAs are commonly expressed in the immune-related organ such as spleen, kidney and gill, also weak expressed in fin and eye. TCR alpha and beta subunit were expressed in brain, whereas gamma and delta were not expressed same tissue. The sequence alignment analysis shows that there are more than 80% sequence homology between TCR subunits. Because it has a high similarity of amino acid sequence to expect similar in function, but expression analysis show that will have may functional diversity due to different time and place of expression.
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제안 방법
In this study, to information of TCR subunits expression during early stage of development, we analyzed the expression pattern of TCR subunits at the three stages of embryos and five laval stages of days post hatching (Fig. 2). As a result, alpha subunit was expressed in the previous hatched stage, while the another subunits were increased at a later larval stage, although a few difference in time.
대상 데이터
The sample was prepared from various tissues including brain, muscle, fin, eye, liver, spleen, kidney, and gill obtained from 2-year-old healthy olive flounder (38 cm±SEM, n=3).
후속연구
The egg and larval stages of marine fishes are the most critical in the successful survival and are extremely sensitive to environmental stress, especially pathogen infection (Rosenthal & Alderice, 1976). Therefore, further study is specifically required of the fish immune system to establish in its own the functions and characteristics, during early step of development which will provide a clear understanding of immune response.
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