[논문]Phycobilisome composition in Chondrus crispus (Gigartinales, Rhodophyta) from a wild type strain and its vegetatively derived green mutant

Cornish, M. Lynn; O' Leary, Stephen J.B.; Garbary, David J.

doi:10.4490/algae.2013.28.1.121

Phycobilisome composition in Chondrus crispus (Gigartinales, Rhodophyta) from a wild type strain and its vegetatively derived green mutant 원문보기

Algae, v.28 no.1, 2013년, pp.121 - 129

Cornish, M. Lynn (Acadian Seaplants Ltd.) , O' Leary, Stephen J.B. (National Research Council Canada, Aquatic & Crop Resource Development Portfolio) , Garbary, David J. (Department of Biology, St. Francis Xavier University)

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Intact phycobilisomes from a wild-type red Chondrus crispus and its vegetatively derived green mutant were isolated by centrifugation through a discontinuous sucrose density gradient. Pigment composition was subsequently characterized by spectrophotometry. Vegetative thalli of the two strains grown together for six months in the laboratory resulted in different pigment profiles. Two pigmented phycobilisome bands appeared in the sucrose gradient of the wild-type alga, a purple coloured one, and a pink one, whereas only a single blue band appeared in the gradient of the green mutant. Spectrophotometric and fluorescence analyses identified the phycobiliprotein composition of the purple band as the typical phycoerythrin-phycocyanin-allophycocyanin complement in the wild-type, but there was no detectable phycoerythrin present in the blue band of the green mutant. Sodium dodecyl sulphate, preparative polyacrylamide gel electrophoresis analysis confirmed the presence of allophycocyanin subunits in all extracts, but firm evidence of an R-phycoerythrin linker polypeptide in the blue band was missing. These results highlight the ability of C. crispus to adapt to a phycoerythrin deficiency by adjusting light harvesting pigment ratios.

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PE is the outermost pigment protein and its function is to increase the light harvesting capacity of the alga. This study has provided a clear explanation for the reduced growth rate of the natural green clone (Cornish unpublished data). An apparent inability to produce a full complement of functionally active PE proteins, accentuated by a deficiency of R-PE linker peptides would account for this inability to maximize light harvesting.

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Absorbance spectra of the pigment proteins isolated by the sucrose density gradient showed that the purple band from the wild type C. crispus consisted primarily of R-PE type I (3 peaks, 498, 540, and 565) (Su et al. 2010b) with some absorption at approximately 620 nm and 650 nm, indicating the presence of PC and APC (Fig. 4).
Pigment content calculated for the coloured bands indicated that the purple band extracted from the red C. crispus had similar PE and APC contents compared to the other samples, but was relatively low in PC content, 20.4, 27.1, and 1.4, respectively. The pink band from this strain had 5.
This study compared the PBP complement of a wild type red alga to that of a green colour mutant that arose vegetatively on a frond of the original wild type plant. Ascertaining that the green mutant was PE deficient, and quantifying the resultant ratios of PC and APC would be instructive in establishing commercial value of the alga as a crop.

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Phycobilisome composition in Chondrus crispus (Gigartinales, Rhodophyta) from a wild type strain and its vegetatively derived green mutant 원문보기

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Phycobilisome composition in Chondrus crispus (Gigartinales, Rhodophyta) from a wild type strain and its vegetatively derived green mutant 원문보기

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