This study screened for radio-resistant strains lactic acid bacteria (LAB) by evaluating their capability to survive exposure to ionizing radiation. Ten strains of LAB - Lactobacillus bulgaricus, Lactobacillus paracasei, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus plantarum, Lactob...
This study screened for radio-resistant strains lactic acid bacteria (LAB) by evaluating their capability to survive exposure to ionizing radiation. Ten strains of LAB - Lactobacillus bulgaricus, Lactobacillus paracasei, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus delbruekii, Lactococcus lactis, Streptococcus thermophilus, Bifidobacterium breve, and Pediocuccos pentosaceus - were selected and subcultuted twice. The LAB was then further cultured for 3 d at $37^{\circ}C$ to reach 7-10 Log colony-forming units (CFU)/mL prior to irradiation and immediately exposed to gamma rays or electron beams with absorbed doses of 0, 1, 2, 3, 4, 5, 6, 8, and 10 kGy. Gamma irradiation gradually decreased the number of the tested viable LAB, and the effect was irradiation dose dependent. A similar effect was found in electron beam-irradiated LAB. Radiation sensitivity of LAB was calculated as $D_{10}$ values, which ranged from 0.26 kGy to 0.9 kGy and 0.5 kGy to 1.44 kGy with exposure to gamma and electron beam irradiation, respectively, in all tested LAB. L. acidophilus was the most resistant to gamma and electron beam irradiation, with $D_{10}$ values of 0.9 kGy and 1.44 kGy, respectively. These results suggest that L. acidophilus might be suitable for the preparation of probiotics as direct-fed microbes for astronauts in extreme space environments.
This study screened for radio-resistant strains lactic acid bacteria (LAB) by evaluating their capability to survive exposure to ionizing radiation. Ten strains of LAB - Lactobacillus bulgaricus, Lactobacillus paracasei, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus delbruekii, Lactococcus lactis, Streptococcus thermophilus, Bifidobacterium breve, and Pediocuccos pentosaceus - were selected and subcultuted twice. The LAB was then further cultured for 3 d at $37^{\circ}C$ to reach 7-10 Log colony-forming units (CFU)/mL prior to irradiation and immediately exposed to gamma rays or electron beams with absorbed doses of 0, 1, 2, 3, 4, 5, 6, 8, and 10 kGy. Gamma irradiation gradually decreased the number of the tested viable LAB, and the effect was irradiation dose dependent. A similar effect was found in electron beam-irradiated LAB. Radiation sensitivity of LAB was calculated as $D_{10}$ values, which ranged from 0.26 kGy to 0.9 kGy and 0.5 kGy to 1.44 kGy with exposure to gamma and electron beam irradiation, respectively, in all tested LAB. L. acidophilus was the most resistant to gamma and electron beam irradiation, with $D_{10}$ values of 0.9 kGy and 1.44 kGy, respectively. These results suggest that L. acidophilus might be suitable for the preparation of probiotics as direct-fed microbes for astronauts in extreme space environments.
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제안 방법
For electron beam irradiation, an ELV4-electron accelerator (energy, 10 MeV; beam power, 570 kW) was used with a beam current of 1 mA. Dosimetry was performed using an alanine dosimeter (5 mm; Bruker Instruments, Germany) for gamma irradiation and a ceric-cerous dosimeter (Bruker Instruments, Germany) for electron beam irradiation, respectively. The error of the total adsorbed doses in the LAB samples was within 5%.
In this study, 10 LAB used in the production of fermented milk products were tested for their capability to survive exposure to ionizing radiation. Among those LAB strains, L.
In this study, LAB used for the production of fermented milk products were tested for their capability to survive exposure to ionizing radiation, and were screened for the selection of radiation-resistant strains suitable for the preparation of probiotics as direct-fed microbes for astronauts in extreme space environments.
대상 데이터
LAB samples were irradiated with gamma or electron beams at doses of 0, 1, 2, 3, 4, 5, 6, 8, and 10 kGy. For gamma irradiation, a cobalt-60 irradiator (IR-79; Nordion International Ltd., Canada) with a source strength of 11.1 PBq was used. For electron beam irradiation, an ELV4-electron accelerator (energy, 10 MeV; beam power, 570 kW) was used with a beam current of 1 mA.
bulgaricus (KCTC3635), Lactobacillus paracasei (KCTC 13169), Lactobacillus casei (KCTC3109), Lactobacillus acidophilus (KCTC3140), Lactobacillus plantarum (KCTC 3103), Lactobacillus delbrueckii (KCTC13730), Lactococcus lactis (KCTC2013), Streptococcus salivarius subsp. thermophilus (KCTC3658), Bifidobacterium breve (KCTC 3419), Pediococcus pentosaceus (KCTC3116) - were obtained from the Korean Collection for Type Cultures (KCTC) at the Korea Research Institute of Bioscience and Biotechnology (Korea).
데이터처리
Statistical analysis of the data was carried out with 1-way analysis of variance using SPSS 19.0 software (SPSS Inc, USA), and significantly different means were compared using the multiple range test of Duncan. A p value of <0.
성능/효과
The rank order of D10 values of electron beamirradiated LAB was L. acidophilus (1.44 kGy) > B. breve (0.82 kGy) > P. pentosaceus (0.80 kGy) > L. casei (0.68 kGy) > L. paracasei (0.64 kGy) > S. thermophilus (0.59 kGy) = L. delbrueckii (0.59 kGy) > L. lactis (0.57 kGy) > L. plantarum (0.50 kGy) = L. bulgaricus (0.50 kGy).
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