Lactic acid bacteria (LAB) in fermented foods have attracted considerable attention recently as treatment options for immune diseases, the incidence of which has been increasing worldwide. The ability of 500 strains of LAB, isolated from kimchi, to induce TNF-${\alpha}$ production in peri...
Lactic acid bacteria (LAB) in fermented foods have attracted considerable attention recently as treatment options for immune diseases, the incidence of which has been increasing worldwide. The ability of 500 strains of LAB, isolated from kimchi, to induce TNF-${\alpha}$ production in peritoneal macrophages was investigated. Lactobacillus plantarum HY7712 most strongly induced TNF-${\alpha}$ production as well as NF-${\kappa}B$ activation. However, HY7712 inhibited NF-${\kappa}B$ activation in LPS-stimulated peritoneal macrophages. When HY7712 was orally treated in cyclophosphamide (CP)-immunosuppressed mice for 5 or 15 days, it reversed the body and spleen weights, blood RBC and WBC levels, and splenocyte and bone marrow cells that were reduced by CP. Orally administered HY7712 increased concanavalin A-induced T cell proliferation to 84.5% of the normal group on day 15, although treatment with CP alone markedly reduced it to 53.7% of the normal group. Furthermore, orally administered HY7712 significantly induced the expressions of IL-2 and IFN-${\gamma}$ in ConA-induced splenic cytotoxic T cells of CP-treated mice. Orally administered HY7712 restored the CP-impaired phagocytosis of macrophages in mice. Orally administered HY7712 also restored the cytotoxicity of NK and cytotoxic T cells derived from spleen and bone marrow against YAC-1 in CP-immunosuppressed mice. Based on these findings, orally administered HY7712 may accelerate the recovery of cyclophosphamide-caused immunosuppression, without evident side effects, by immunopotentiating NK and Tc cells, and may provide a mechanistic basis for using HY7712 as an alternative means in lessening chemotherapyinduced immunosuppression in cancer patients.
Lactic acid bacteria (LAB) in fermented foods have attracted considerable attention recently as treatment options for immune diseases, the incidence of which has been increasing worldwide. The ability of 500 strains of LAB, isolated from kimchi, to induce TNF-${\alpha}$ production in peritoneal macrophages was investigated. Lactobacillus plantarum HY7712 most strongly induced TNF-${\alpha}$ production as well as NF-${\kappa}B$ activation. However, HY7712 inhibited NF-${\kappa}B$ activation in LPS-stimulated peritoneal macrophages. When HY7712 was orally treated in cyclophosphamide (CP)-immunosuppressed mice for 5 or 15 days, it reversed the body and spleen weights, blood RBC and WBC levels, and splenocyte and bone marrow cells that were reduced by CP. Orally administered HY7712 increased concanavalin A-induced T cell proliferation to 84.5% of the normal group on day 15, although treatment with CP alone markedly reduced it to 53.7% of the normal group. Furthermore, orally administered HY7712 significantly induced the expressions of IL-2 and IFN-${\gamma}$ in ConA-induced splenic cytotoxic T cells of CP-treated mice. Orally administered HY7712 restored the CP-impaired phagocytosis of macrophages in mice. Orally administered HY7712 also restored the cytotoxicity of NK and cytotoxic T cells derived from spleen and bone marrow against YAC-1 in CP-immunosuppressed mice. Based on these findings, orally administered HY7712 may accelerate the recovery of cyclophosphamide-caused immunosuppression, without evident side effects, by immunopotentiating NK and Tc cells, and may provide a mechanistic basis for using HY7712 as an alternative means in lessening chemotherapyinduced immunosuppression in cancer patients.
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제안 방법
First, we selected HY7712, which induced TNF-α production via NF-κB activation in the macrophage, but inhibited NF-κB activation in LPS-stimulated macrophages, and investigated its immunopotentiating effect in cyclophosphamideimmunosuppressed mice.
Mice were divided into 9 groups (8 mice per group) 24 h after the final injection of cyclophosphamide and received Lactobacillus plantarum HY7712 (1×108 , 1×109 , or 1×1010 CFU), orally administered once a day for 5 or 15 days and levamisole (2.5 mg/kg) as a positive control or vehicle as a negative control.
To evaluate the immunopotentiating effect of HY7712 in vivo, we administered it to CP-immunosuppressed mice and measured the weights of the body and spleen and the numbers of peripheral RBC and WBC, splenocytes, and BMC (Fig. 2). Treatment with CP significantly reduced body weight to 78.
To isolate immunopotentiating LAB, we selected LAB from kimchi, a Korea fermented food, and measured their TNF-α productivity and NF-κB activation in peritoneal macrophages stimulated with or without LPS.
대상 데이터
LAB were selected from the Korea Yakult Company Collection (Gyunggi prefecture, Korea), of which 500 strains were LAB isolated from kimchi by using MRS agar.
데이터처리
All data were expressed as the mean ± standard deviation, with statistical significance analyzed using one-way ANOVA followed by a Student-Newman-Keuls test.
이론/모형
The effect of HY7712 on the phagocytosis of macrophages isolated from mice was measured by the neutral red uptake method (Fig. 5). Treatment with CP in mice significantly reduced the phagocytosis of macrophages.
1 ml) with or without LPS (50 ng/ml). Their cytotoxicities were measured using the crystal violet method.
성능/효과
Treatment with CP alone significantly reduced the NK cell and cytotoxic T cell cytotoxicities on days 5 and 15, compared with normal mice. Treatment with HY7712 restored the cytotoxicity of NK and cytotoxic T cells derived from spleen against YAC-1 cells to 98.2% and 99.1% of the normal mice on day 5, and 98.1% and 99.6% on day 15, respectively. The cytotoxicity of NK and cytotoxic T cells from bone marrow that was reduced by CP was reversed to 98.
Treatments with HY7712 (1×1010 CFU) increased concanavalin A-induced T cell proliferation to 80.3% and 84.5% of the normal group on days 5 and 15, respectively, whereas the T cell proliferation in the vehicle groups was significantly lower than the normal group.
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