[논문]Inhibition of Proinflammatory Cytokine Generation in Lung Inflammation by the Leaves of Perilla frutescens and Its Constituents

Lim, Hun Jai; Woo, Kyeong Wan; Lee, Kang Ro; Lee, Sang Kook; Kim, Hyun Pyo

doi:10.4062/biomolther.2013.088

[국내논문] Inhibition of Proinflammatory Cytokine Generation in Lung Inflammation by the Leaves of Perilla frutescens and Its Constituents 원문보기

Biomolecules & therapeutics, v.22 no.1, 2014년, pp.62 - 67

Lim, Hun Jai (College of Pharmacy, Kangwon National University) , Woo, Kyeong Wan (College of Pharmacy, Sungkyunkwan University) , Lee, Kang Ro (College of Pharmacy, Sungkyunkwan University) , Lee, Sang Kook (College of Pharmacy, Seoul National University) , Kim, Hyun Pyo (College of Pharmacy, Kangwon National University)

Abstract ▼ AI-Helper

This study was designed to find some potential natural products and/or constituents inhibiting proinflammatory cytokine generation in lung inflammation, since cytokines such as tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interleukin-6 (IL-6) are pivotal for provoking airway inflammation. In our preliminary screening procedure, the 70% ethanol extract of the leaves of Perilla frutescens (PFE) was found to clearly inhibit TNF-${\alpha}$ production in the lung at 100 mg/kg, after intranasal lipopolysaccharide treatment of mice. Based on this result, ten constituents including phenylpropanoids (allyltetramethoxybenzene, caffeic acid, dillapiole, elemicin, myristicin, nothoapiole, rosmarinic acid methyl ester, rosmarinic acid) and monoterpenes (perilla aldehyde and perilla ketone) were successfully isolated from the extract. Among them, elemicin and myristicin were found for the first time to concentration-dependently inhibit IL-$1{\beta}$-treated IL-6 production from lung alveolar epithelial cells (A549) at concentrations of $10-100{\mu}M$. These findings suggest that the phenylpropanoids including elemicin and myristicin have the potential to be new inhibitory agents against lung inflammation and they may contribute, at least in part, to the inhibitory activity of PFE on the lung inflammatory response.

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문제 정의

And the results show that PFE and the constituents possess meaningful activity. To the best of our knowledge, this is the first report demonstrating the potential therapeutic effects of PFE and its constituents on lung inflammation.

대상 데이터

Lee (Dongguk University, Gyoungju, Korea). A voucher specimen (SKKU NPL 1207) was deposited in the herbarium of the School of Pharmacy, Sungkyunkwan University, Suwon, Korea.
The search for the active compounds yielded three phenylpropanoids including elemicin, myristicin and rosmarinic acid. These compounds clearly inhibited IL-6 production in lung epithelial cells.

데이터처리

One way ANOVA followed by Dunnett’s test was used to determine the statistical significance.

성능/효과

In conclusion, all results of the present study demonstrated that the leaves of P. frutescens (L.) Britton var. acuta Kudo inhibited lung inflammation in vivo.

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