[논문]Ethanol Extract of Ulmus pumila Ameliorates Heat Stress through the Induction of Heat Shock Proteins Expression in RAW264.7 Macrophage Cells

dela Cruz, Joseph; Byambaragchaa, Munkhzaya; Choi, Seok-Geun; Hwang, Seong-Gu

doi:10.11109/jaes.2014.20.4.147

Ethanol Extract of Ulmus pumila Ameliorates Heat Stress through the Induction of Heat Shock Proteins Expression in RAW264.7 Macrophage Cells 원문보기

한국축산시설환경학회지 = Journal of animal environmental science, v.20 no.4, 2014년, pp.147 - 154

dela Cruz, Joseph (Department of Animal Life and Environmental Science, Hankyong National University) , Byambaragchaa, Munkhzaya (Department of Animal Life and Environmental Science, Hankyong National University) , Choi, Seok-Geun (Department of Animal Life and Environmental Science, Hankyong National University) , Hwang, Seong-Gu (Department of Animal Life and Environmental Science, Hankyong National University)

Abstract ▼ AI-Helper

Heat stress is a significant burden to animal production in most areas of the world. Improving our knowledge of physiological and metabolic mechanisms of acclimation may contribute to the development of procedures that may help to maintain health and production efficiency under hot temperature. The effect of Ulmus pumila (UP) extract in inducing Heat Shock Proteins (HSPs) expression in heat-stressed RAW264.7 macrophage cells was investigated. Cell viability assay showed a dose dependent increase in cells after treatment with UP for 24 hours. RT-PCR and western blot analysis showed that increasing concentrations of UP induce the expression of Heat Shock Factor 1 (HSF1) and dose dependently upregulated the expression of Heat shock protein 70 (Hsp70) and Hsp90. LPS-induced nitric oxide was dose-dependently reduced while phagocytic activity greatly recovered with UP treatment. These data demonstrated that UP can be a potential candidate in the development of cytoprotective agent against heat stress.

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제안 방법

But the cytoprotective effect of Ulmus pumila against heat stress has not been reported so far. In this study our aim was to determine the cytoprotective ability of Ulmus pumila ethanol extract on the heat stressed Raw264.7 cells, as well as to reveal a potential gene transduction which are involved in cytoprotective function of this plant extract.
Thus, phagocytosis is an important indicator of macrophage effector activity. In this study, we evaluated the effect of heat stress on the phagocytic activity of RAW264.7 macrophage cells.
7 cells. To further investigate the mechanism of action behind the cytoprotective activity of UP, we analyzed the expressions of heat stress related genes and proteins.

대상 데이터

RAW264.7 cells were obtained from the Korean Cell Line Bank (KCLB). The cells were maintained in Dulbecco’s Modified Eagle’s medium (DMEM), supplemented with 10% heat inactivated fetal bovine serum (FBS), penicillin (100 U/ml), streptomycin (100 mg/ml), and 3.

데이터처리

All quantitative data are representative of at least three independent experiments and the results were expressed as means + S.D. Differences between means were evaluated using ANOVA test (one-way) followed by Duncan’s Multiple Range Test.

이론/모형

Cell viability was quantified by CCK-8 assay. Briefly, the cells were plated in 96-well plates at a density of 1×10⁵ cells/mL and allowed to adhere at 37℃ for 3h.

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