개의 아토피성 피부염에서 Dermatophagoides farinae의 항원을 immunoblot을 통해 확인하였다. 실험은 D. farinae에 의해 아토피성 피부염이 유발된 16마리와 임상적으로 건강한 10마리 개의 혈청으로 수행하였으며, SDS-PAGE를 실시하여 D. farinae 추출물의 항원들이 15에서 220 kDa 범위 내에 존재하는 것을 관찰하였다. Immunoblot 결과, 임상적으로 건강한 10마리 개의 혈청에서는 단백띠가 보이지 않았으며, D. farinae에 의해 아토피성 피부염이 유발된 16마리 개의 혈청을 이용한 실험에서는 14마리에서 109 kDa, 13마리에서 44 kDa, 11마리에서 177 kDa, 7마리에서 27 kDa, 5마리에서 75 kDa, 3마리에서 15 kDa의 단백띠가 관찰되었다. 즉, D. farinae에 의해 아토피성 피부염이 유발된 개체 중 50% 이상에서 관찰되는 주요 항원은 177 kDa, 109 kDa, 44 kDa 항원이었다. 본 실험과 같이 주요 항원들을 확인하는 이러한 연구는 아토피성 피부염에 대한 새로운 면역 치료의 전략과 제제의 개발에 기여할 수 있을 것으로 생각된다.
개의 아토피성 피부염에서 Dermatophagoides farinae의 항원을 immunoblot을 통해 확인하였다. 실험은 D. farinae에 의해 아토피성 피부염이 유발된 16마리와 임상적으로 건강한 10마리 개의 혈청으로 수행하였으며, SDS-PAGE를 실시하여 D. farinae 추출물의 항원들이 15에서 220 kDa 범위 내에 존재하는 것을 관찰하였다. Immunoblot 결과, 임상적으로 건강한 10마리 개의 혈청에서는 단백띠가 보이지 않았으며, D. farinae에 의해 아토피성 피부염이 유발된 16마리 개의 혈청을 이용한 실험에서는 14마리에서 109 kDa, 13마리에서 44 kDa, 11마리에서 177 kDa, 7마리에서 27 kDa, 5마리에서 75 kDa, 3마리에서 15 kDa의 단백띠가 관찰되었다. 즉, D. farinae에 의해 아토피성 피부염이 유발된 개체 중 50% 이상에서 관찰되는 주요 항원은 177 kDa, 109 kDa, 44 kDa 항원이었다. 본 실험과 같이 주요 항원들을 확인하는 이러한 연구는 아토피성 피부염에 대한 새로운 면역 치료의 전략과 제제의 개발에 기여할 수 있을 것으로 생각된다.
Dermatophagoides farinae plays important role in the pathogenesis of canine atopic dermatitis as environmental allergens. Also, many studies revealed that D. farinae was the main causative allergen for Korean dogs with atopic dermatitis. To identify major allergens of D. farinae in Korean atopic dog...
Dermatophagoides farinae plays important role in the pathogenesis of canine atopic dermatitis as environmental allergens. Also, many studies revealed that D. farinae was the main causative allergen for Korean dogs with atopic dermatitis. To identify major allergens of D. farinae in Korean atopic dogs allergic to D. farinae by immunoblot using commercial allergenic extracts, 26 dogs from two groups were enrolled in the study. Control group consists of 10 dogs with no clinical signs of disease and atopic group consists of 16 dogs diagnosed as atopic dermatitis. Sera from Korean dogs with atopic dermatitis showed six allergens of D. farinae extract by procedure of immunoblot. The molecular weights of identifying protein bands were 177, 109, 75, 44, 27, 15 kDa. The major allergens showing reactivity with greater than 50% of atopic dogs were detected at approximately 44, 109 and 177 kDa. Subsequent investigations will be carried out to verify the identity of the allergens detected in this study.
Dermatophagoides farinae plays important role in the pathogenesis of canine atopic dermatitis as environmental allergens. Also, many studies revealed that D. farinae was the main causative allergen for Korean dogs with atopic dermatitis. To identify major allergens of D. farinae in Korean atopic dogs allergic to D. farinae by immunoblot using commercial allergenic extracts, 26 dogs from two groups were enrolled in the study. Control group consists of 10 dogs with no clinical signs of disease and atopic group consists of 16 dogs diagnosed as atopic dermatitis. Sera from Korean dogs with atopic dermatitis showed six allergens of D. farinae extract by procedure of immunoblot. The molecular weights of identifying protein bands were 177, 109, 75, 44, 27, 15 kDa. The major allergens showing reactivity with greater than 50% of atopic dogs were detected at approximately 44, 109 and 177 kDa. Subsequent investigations will be carried out to verify the identity of the allergens detected in this study.
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문제 정의
The 44, 109 and 177 kDa allergens appeared at high frequency, so these allergens will be helpful for diagnosis and therapy of canine atopic dermatitis. Further studies as cDNA and/or N-terminal amino acid sequencing are needed to understand the biological, chemical and structural properties of these allergens observed in this investigation.
Therefore, the purpose of this study was to identify major allergens of D. farinae in Korean atopic dogs allergic to D. farinae by immunoblot using commercial allergenic extracts.
성능/효과
farinae extract is shown in Fig 1. Analysis of the D. farinae extract by SDS-PAGE under reducing condition revealed protein bands at approximately 15 kDa, 20 kDa, 27 kDa, 32 kDa, 44 kDa, 60 kDa, 70 kDa, 109 kDa, 177 kDa, 220 kDa.
Also, another report identified high IgE-reactivity to Der f 2 from Japanese dogs (22). In this investigation, Der f 1 and Der f 2 were recognized by fewer than 50% of D. farinae-sensitized dogs, so it is determined that Der f 1 and Der f 2 are not major allergens in Korea.
In this study, the results of immunoblot assay using the D. farinae extract showed bands with different molecular weights ranging from 15 to 177 kDa.
참고문헌 (23)
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-254.
Cunha VES, Silva MH, Faccini JLH. Serological identification of house dust mite allergens in dogs with atopic dermatitis. Pesq Vet Bras 2012; 32: 917-921.
Favrot C, Steffan J, Seewald W, Picco F. A prospective study on the clinical features of chronic canine atopic dermatitis and its diagnosis. Vet Dermatol 2010; 21: 23-31.
Fujikawa A, Ishimaru N, Seto A, Yamada H, Aki T, Shigeta S, Wada T, Jyo T, Murooka Y, Oka S, Ono K. Cloning and characterization of a new allergen, Mag 3, from the house dust mite, Dermatophagoides fariane: cross-reactivity with high-molecular-weight allergen. Mol Immunol 1996; 33: 311-319.
Fujikawa A, Uchida K, Yanagidani A, Kawamoto S, Aki T, Shigeta S, Wada T, Suzuki O, Jyo T, Ono K. Altered antigenicity of M-177, a 177-kDa allergen from the house dust mite Dermatophagoides fariane, in stored extract. Clin Exp Allergy 1998; 28: 1549-1558.
Hillier A, Kwochka KW, Pinchbeck LR. Reactivity to intradermal injection of extracts of Dermatophagoides farinae, Dermatophagoides pteronyssinus, house dust mite mix, and house dust in dogs suspected to have atopic dermatitis: 115 cases (1996-1998). J Am Vet Med Assoc 2000; 217: 536-540.
Le Mao J, Mayer C, Peltre G, Desvaux FX, David B, Weyer A, Senechal H. Mapping of Dermatophagoides farinae mite allergens by two-dimensional immunoblotting. J Allergy Clin Immunol 1998; 66: 203-223.
Lian TM, Halliwell RE. Allergen-specific IgE and IgGd antibodies in atopic and normal dogs. Vet Immunol Immunopathol 1998; 66: 203-223.
Masuda K, Tsujimoto H, Fujiwara S, Kurata K, Hasegawa A, Yasueda H, Yamashita K, DeBoer DJ, de Weck AL, Sakaguchi M. IgE sensitivity and cross-reactivity to crude and purified mite allergens (Der f 1, Der f 2, Der p 1, Der p 2) in atopic dogs sensitive to Dermatophagoides mite allergens. Vet Immunol Immunopathol 1999; 72: 303-313.
McCall C, Hunter S, Stedman K, Weber E, Hillier A, Bozic C, Rivoire B, Olivry T. Characterization and cloning of a major high molecular weight house dust mite allergen (Der f 15) for dogs. Vet Immunol Immunopathol 2001; 78: 231-247.
Noli C, Bernadina WE, WillemseT. The significance of reactions to purified fractions of Dermatophagoides pteronyssinus and Dermatophagoides farinae in canine atopic dermatitis. Vet Immunol Immunopathol 1996; 52: 147-157.
Nuttall TJ, Hill PB, Bensignor E, Willemse T. House dust and forage mite allergens and their role in human and canine atopic dermatitis. Vet Dermatol 2006; 17: 223-235.
Platts-Mills TA, Vervloet D, Thomas WR, Aalverse RC, Chapman MD. Indoor allergens and asthma: report of the Thir d International Workshop. J Allergy Clin Immunol 1997; 100: S2-24.
Song KH, Lee JY, Liu J, Lee SE, Park SJ, Kim DH. Prevalence of Causative Allergens on Canine Atopic Dermatitis in Daejeon Area. J Vet Clin 2005; 22: 26-30.
Sture GH, Halliwell RE, Thoday KL, van den Broek AH, Henfrey JI, Lloyd DH, Mason IS, Ferguson E. Canine atopic disease: the prevalence of positive intradermal skin tests at two sites in the north and south of Great Britain. Vet Immunol Immunopathol 1995; 44: 293-308.
Weber E, Hunter S, Stedman K, Dreitz S, Olivry T, Hillier A, McCall C. Identification, characterization, and cloning of a complementary DNA encoding a 60-kd house dust mite allergen (Der f 18) for human beings and dogs. J Allergy Clin Immunol 2003; 112: 79-86.
Yamashita K, Fujiwara C, Azuma R, Sawazaki T, Nakao Y, Hasegawa A. Determination of antigenic proteins of housedust mites in 90 dogs suffering from atopic dermatitis. J Vet Med Sci 2002; 64: 673-676.
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