[논문]Classification of the Efficacy of Herbal Medicine Alterations in Neuronal Hypoxia Models through Analysis of Gene Expression

Hwang, Joo-Won; Shin, Gil-Cho; Moon, Il-Su

doi:10.13048/jkm.14042

[국내논문] Classification of the Efficacy of Herbal Medicine Alterations in Neuronal Hypoxia Models through Analysis of Gene Expression 원문보기

Journal of Korean Medicine = 대한한의학회지, v.35 no.4, 2014년, pp.36 - 51

Hwang, Joo-Won (Dept. of Internal Medicine, College of Korean Medicine, Dongguk University) , Shin, Gil-Cho (Dept. of Internal Medicine, College of Korean Medicine, Dongguk University) , Moon, Il-Su (Dept. of Anatomy, College of Medicine, Dongguk University)

Abstract ▼ AI-Helper

Objectives: cDNA microarray is an effective method to snapshot gene expression. Functional clustering of gene expressions can identify herbal medicine mechanisms. Much microarray data is available for various herbal medicines. This study compares regulated genes with herbal medicines to evaluate the nature of the drugs. Methods: Published microarray data were collected. Total RNAs were prepared from dissociated hippocampal dissociate cultures which were given hypoxic shock in the presence of each herbal medicine. Up- or downregulated genes higher than Global M value 0.5 were selected, clustered in functional groups, and compared with various herbal treatments. Results: 1. Akt2 was upregulated by Acorus gramineus SOLAND, Arisaema amurense var. serratum $N_{AKAI}$ and Coptis chinensis $F_{RANCH}$, and they belong to Araceae herb. 2. Nf-${\kappa}b1$, Cd5, $Gn{\gamma}7$ and Sgne1 were upregulated by Arisaema amurense var. serratum $N_{AKAI}$, Coptis chinensis $F_{RANCH}$ and Rheum coreanum $N_{AKAI}$. 3. Woohwangcheongsim-won, Sohaphyang-won and Scutellaria baicalensis $G_{EORGI}$ downregulated Scp2 and upregulated Tsc2. Woohwangcheongsim-won and Sohaphyang-won upregulated Hba1 and downregulated Myf6. 4. Sohaphyang-won and Scutellaria baicalensis $G_{EORGI}$ downregulated Slc12a1. 5. Woohwangcheongsim-won and Arisaema amurense var. serratum $N_{AKAI}$ upregulated $Rar{\alpha}$, Woohwangcheongsim-won and Coptis chinensis $F_{RANCH}$ downregulated Rab5a and $Pdgfr{\alpha}$, and Woohwangcheongsim-won and Rheum coreanum $N_{AKAI}$ upregulated $Plc{\gamma}1$ and downregulated Pla2g1b and Slc10a1. Conclusions: By clustering microarray, genes are commonly identified to be either up- or downregulated. These results will provide new information to understand the efficacy of herbal medicines and to classify them at the molecular level.

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가설 설정

1) A total of 4 types of genes were identical. Table 10 shows the result of the analysis.

제안 방법

The author analyzed experimental studies that observed expressed genes from herbal medicines or extracts in neurons where apoptosis has occurred under hypoxia and interpreted the influence on gene function related to the medicine’s neuron protection and anti-oxidation from the point of view of Korean medicine.
This study limited its target to experimental research that used clustering to investigate altered expression of genes through microarray after inducing apoptosis in neurons under hypoxia and treating with herbal medicines or extracts.
There recently has been a lot of research that tries to find the characteristics of herbal medicines or extracts through cDNA microarray. This study compared and analyzed research reports limited to those that observed gene expression alteration through the clustering method in the neuronal hypoxia model among research on the gene expression of herbal medicines using the cDNA microarray method. Target herbal medicines were Acorus gramineus S_OLAND⁷⁾, Arisaema amurense var.

성능/효과

Table 7 shows the result of the analysis. There were 2 types of genes related to apoptosis, 3 types of genes related to cell cycle, 10 types of genes related to response to stress, 11 types of genes related to signal transduction, 5 types of genes related to transcription, 17 types of genes related to physiological process, and 7 types of genes related to immune response.
serratum N_AKAI, Pinellia ternata B_REIT., Scutellaria baicalensis G_EORGI, Coptis chinensis F_RANCH, Woohwangcheongsim-won, and Sohaphyang-won because of excellent ROS removal ability, and they were helpful in maintaining MMP. In the case of Acorus gramineus S_OLAND, Arisaema amurense var.
There was no gene that was expressed commonly between Scutellaria baicalensis G_EORGI and Coptis chinensis F_RANCH in this study. This result lends the possibility that Coptis chinensis F_RANCH and Scutellaria baicalensis G_EORGI may function in a different mechanism rather than the clear heat function in the same mechanism. Future research on this aspect is necessary.
When there is low energy, AMP-activated protein kinase (AMPK) phosphorylates Tsc2 and protects the cell from apoptosis³³⁾. In this study, in all Woohwangcheongsim -won, Sohaphyang-won and Scutellaria baicalensis G_EORGI, Tsc2 was upregulated, and this is identical to the apoptosis inhibiting function. Meanwhile, Scp2 is a DNA combined protein that supports the chromosome structure in the former part of meiosis.

후속연구

Through this study, genes that are commonly expressed were identical in Scutellaria baicalensis G_EORGI which is an ingredient medicine of Woohwangcheongsim-won and Woohwangcheongsim-won and Sohaphyang-won. Future research should study the gene that is expressed in the identical direction as the function research of the ingredient medicine of Woohwangcheongsim-won or Sohaphyang-won.

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