[논문]Efficacy and Reusability of Commercial Adsorbent for Isolation of Proanthocyanidins from Hot Water Extract of Pinus radiata Bark

Mun, Sung Phil

doi:10.5658/wood.2014.42.2.207

[국내논문] Efficacy and Reusability of Commercial Adsorbent for Isolation of Proanthocyanidins from Hot Water Extract of Pinus radiata Bark 원문보기

목재공학 = Journal of the Korean wood science and technology, v.42 no.2, 2014년, pp.207 - 213

Mun, Sung Phil (Department of Wood Science and Technology, Chonbuk National University)

Abstract ▼ AI-Helper

This study was carried out to investigate the feasibility and usefulness of a commercial synthetic adsorbent, Diaion HP 20, for the isolation of proanthocyanidins (PAs) from hot water extract (HWE) prepared from Pinus radiata bark. Most ultraviolet (UV) absorbing materials in HWE were adsorbed onto Diaion HP 20 and easily recovered by simple ethanol (EtOH) washing. More than 50% of the UV absorbing materials were adsorbed within 20 minutes, and the adsorption equilibrium was reached within 3 h. The recovered materials from Diaion HP 20 were mostly composed of PAs, but some monomeric flavonoids such as taxifolin and unidentified hydrophobic materials were also adsorbed. The impurities such as carbohydrates and inorganic materials contained in HWE were mostly removed by Diaion HP 20. The adsorption ability of the UV absorbing materials onto Diaion HP 20 was almost the same as the first cycle even after 14 times of repeating cycles of adsorption and desorption. The radical scavenging ability of the recovered materials from the adsorbent was slightly higher than that of the pure PAs prepared by Sephadex LH 20 from the same HWE.

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문제 정의

Therefore, these impurities in HWE should be removed not only for the production of pure PAs; but also for further useful applications, such as health supplements, food additives, and medical supplies. The aim of this study was to explore that PAs can be effectively isolated from HWE using Diaion HP 20. We also investigated the reusability of Diaion HP 20, which can provide useful information for the commercialization of PAs isolation and purification method.

대상 데이터

This study was carried out with the support of “Forest Science & Technology Projects (Project No. S211313L010110)” provided by Korea Forest Service.

성능/효과

The fresh HWE was in the state of slight cloudy solution but some precipitations start to appear as time passed. It was thought that the slight decrease in the adsorption yield as the repeating cycle is increased is due to the aggregation of some high-molecular-weight PAs having a poor solubility in water. Even after repeating 14 cycles of adsorption and desorption, Diaion HP 20 still kept all most the same recovery efficiency as the first cycle, and the average adsorption yield was maintained at about 80%.

참고문헌 (15)

Bagchi, D., M. Bagachi, S. J. Stohs, D. K. Das, S. D. Ray, C. A. Ray, S. S. Joshi, and H. G. Pruess. 2000. Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention, Toxicol. 148: 187-197.

상세보기
Diaion ${\circledR}$ , Product Line Brochure, Mitsubishi Chemical Coporation, www.diaion.com.
Kohama, T., N. Suzuki, S. Ohno, and M. Inoue. 2004. Analgesic efficacy of French maritime pine bark extract in dysmenorrheal: an open clinical trial, J. Reprod. Med. 49: 828-832.
Ku, C. S. and S. P. Mun. 2007. Characterization of proanthocyanidin in hot water extract isolated from Pinus radiata bark, Wood Sci. Technol. 41(3): 235-247.

상세보기
Ku, C. S. and S. P. Mun. 2008. Antioxidant properties of monomeric, oligomeric and polymeric fractions in hot water extract from Pinus radiata bark, Wood Sci. Technol. 42(1): 47-60.

상세보기
Ku, C. S. and S. P. Mun. 2008. Stabilization of extracellular matrix by Pinus radiata bark extracts with different molecular weight distribution against enzymatic degradation and radicals, Wood Sci. Technol. 42(5): 427-436.

상세보기
Ku, C. S., S. P. Mun, and J. P. Jang. 2011. Effects of water extraction temperatures on the yield, molecular weight, and antioxidant activity of proanthocyanidins extracted from Pinus radiata bark, Forest Prod. J. 61(4): 321-325.

상세보기
Mun, S. P. and C. S. Ku. 2006. Characterization of low molecular weight polyphenols from pine (Pinus radiata) bark, Food Sci. Biotechnol. 15(3): 424-430.

원문보기 상세보기
Mun, S. P., I. A. Gilmour, and P. J. Jordan. 2006. Effect of organic sulfonic acids as catalysts during phenol liquefaction of Pinus radiata bark, J. Ind. Eng. Chem. 12: 720-726.

상세보기
Mun, S. P., J. P. Jang, and M. G. Kim. 2012. Effect of Pinus radiata bark polyphenols on phenol liquefaction of cellulose in the presence of p-toluene sulfonic acid catalyst, Forest Products J. 62(3): 234-239.

상세보기
Park, I. J., S. Y. Cha, M. Kang, Y. S. So, H. G. Go, S. P. Mun, K. S. Ryu, and H. K. Jang. 2011. Effect of Proanthocyanidin-rich extract from Pinus radiata bark on immune responses of specificpathogen- free White Leghorn chickens, Poult. Sci. 90: 977-982.

상세보기
Prior, R. L. and L. Gu. 2005. Occurrence and biological significance of proanthocyanidins in the American diet, Phytochem. 66: 2264-2280.

상세보기
Shan, B., Y. Z. Cai, J. D. Brooks, and H. Corke. 2007. Antibacterial properties and major bioactive components of cinnamon stick (Cinnamomum burmannii): activity against food borne pathogenic bacteria, J. Agric. Food Chem. 55: 5484-5490.

상세보기
Stevens, J. F., C. L. Miranda, K. R. Wolthers, M. Schimerlik, M. L. Deinzer, and D. R. Buhler. 2002. Identification and in vitro biological activities of hop proanthocyanidins inhibition of nNOS activity and scavenging of reactive nitrogen species, J. Agric. Food Chem. 50: 3435-3443.

상세보기
Torras, M. A. C., C. A. Faura, F. Schonlau, and P. Rohdewald. 2005. Antimicrobial activity of Pycnogenol, Phytother. Res. 19: 647-648.

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