Protaetia brevitarsis seulensis (Kolbe) is widely used in Korea, as a protein-rich, alternate, functional food with pharmacological benefits. In addition to anti-oxidant properties, the larvae of P. b. seulensis also show positive effects against hepatic disorder and diabetes; therefore, P. b. seule...
Protaetia brevitarsis seulensis (Kolbe) is widely used in Korea, as a protein-rich, alternate, functional food with pharmacological benefits. In addition to anti-oxidant properties, the larvae of P. b. seulensis also show positive effects against hepatic disorder and diabetes; therefore, P. b. seulensis larvae are being reared on a large scale in Korea. We evaluated reared larvae of P. b. seulensis from Gyeong-gi in Korea. Using 16SrRNA PCR, electro-microscopy, and bioassay techniques, we found that the larvae harbored Spo-1, a bacterium identified as the insect pathogen Serratia marcescens. Therefore, we highlight the use of this insect as an alternate food and the need for its sanitary rearing conditions, as contamination may affect public health.
Protaetia brevitarsis seulensis (Kolbe) is widely used in Korea, as a protein-rich, alternate, functional food with pharmacological benefits. In addition to anti-oxidant properties, the larvae of P. b. seulensis also show positive effects against hepatic disorder and diabetes; therefore, P. b. seulensis larvae are being reared on a large scale in Korea. We evaluated reared larvae of P. b. seulensis from Gyeong-gi in Korea. Using 16SrRNA PCR, electro-microscopy, and bioassay techniques, we found that the larvae harbored Spo-1, a bacterium identified as the insect pathogen Serratia marcescens. Therefore, we highlight the use of this insect as an alternate food and the need for its sanitary rearing conditions, as contamination may affect public health.
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제안 방법
The oral treatments doses comprised of 1011/50 mL volume of bacteria mixed with fermented sawdust as food to 10 larvae in rearing box. Bioassay tests with oral or injection treatment were performed in triplicate, using 7 to 10 healthy, second- or third-stage larvae per test.
brevitarsis seulensis. In this work, we have identified the bacterium Spo-1, newly isolated from P. brevitarsis seulensis from Gyeonggi Province, as Serratia marcescens and verified its pathogenicity and toxicity via a bioassay involving intracoelomic injection or oral treatment of P. brevitarsis seulensis larvae with bacterial suspension.
대상 데이터
seulensis that appeared to have amber disease. The larvae were collected from Gyeong-gi Province, Korea. Intracolelomic injection assay was done to evaluate the fatal effects of Spo-1 on the P.
성능/효과
The 16SrRNA sequences were highly conserved and PCR products contained approximately 1500 bp. The 16SrDNA sequence of isolate Spo-1 showed 99% similarity to S. marcescens, NCBI accession number NR 102509.1. (Table 2).
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