Jang, Young-Eun
(Department of Conservative Dentistry, Chonnam National University School of Dentistry and Dental Science Research Institute)
,
Lee, Bin-Na
(Department of Conservative Dentistry, Chonnam National University School of Dentistry and Dental Science Research Institute)
,
Koh, Jeong-Tae
(Department of Pharmacology and Dental Therapeutics, Chonnam National University School of Dentistry and Dental Science Research Institute)
,
Park, Yeong-Joon
(Department of Dental Materials, Chonnam National University School of Dentistry and Dental Science Research Institute)
,
Joo, Nam-Eok
(University of Michigan School of Dentistry)
,
Chang, Hoon-Sang
(Department of Conservative Dentistry, Chonnam National University School of Dentistry and Dental Science Research Institute)
,
Hwang, In-Nam
(Department of Conservative Dentistry, Chonnam National University School of Dentistry and Dental Science Research Institute)
,
Oh, Won-Mann
(Department of Conservative Dentistry, Chonnam National University School of Dentistry and Dental Science Research Institute)
,
Hwang, Yun-Chan
(Department of Conservative Dentistry, Chonnam National University)
Objectives: The aim of this study was to evaluate the cytotoxicity, setting time and compressive strength of MTA and two novel tricalcium silicate-based endodontic materials, Bioaggregate (BA) and Biodentine (BD). Materials and Methods: Cytotoxicity was evaluated by using a 2,3-bis(2-methoxy-4-nitro...
Objectives: The aim of this study was to evaluate the cytotoxicity, setting time and compressive strength of MTA and two novel tricalcium silicate-based endodontic materials, Bioaggregate (BA) and Biodentine (BD). Materials and Methods: Cytotoxicity was evaluated by using a 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide (XTT) assay. Measurements of 9 heavy metals (arsenic, cadmium, chromium, copper, iron, lead, manganese, nickel, and zinc) were performed by inductively coupled plasma-mass spectrometry (ICP-MS) of leachates obtained by soaking the materials in distilled water. Setting time and compressive strength tests were performed following ISO requirements. Results: BA had comparable cell viability to MTA, whereas the cell viability of BD was significantly lower than that of MTA. The ICP-MS analysis revealed that BD released significantly higher amount of 5 heavy metals (arsenic, copper, iron, manganese, and zinc) than MTA and BA. The setting time of BD was significantly shorter than that of MTA and BA, and the compressive strength of BA was significantly lower than that of MTA and BD. Conclusions: BA and BD were biocompatible, and they did not show any cytotoxic effects on human periodontal ligament fibroblasts. BA showed comparable cytotoxicity to MTA but inferior physical properties. BD had somewhat higher cytotoxicity but superior physical properties than MTA.
Objectives: The aim of this study was to evaluate the cytotoxicity, setting time and compressive strength of MTA and two novel tricalcium silicate-based endodontic materials, Bioaggregate (BA) and Biodentine (BD). Materials and Methods: Cytotoxicity was evaluated by using a 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide (XTT) assay. Measurements of 9 heavy metals (arsenic, cadmium, chromium, copper, iron, lead, manganese, nickel, and zinc) were performed by inductively coupled plasma-mass spectrometry (ICP-MS) of leachates obtained by soaking the materials in distilled water. Setting time and compressive strength tests were performed following ISO requirements. Results: BA had comparable cell viability to MTA, whereas the cell viability of BD was significantly lower than that of MTA. The ICP-MS analysis revealed that BD released significantly higher amount of 5 heavy metals (arsenic, copper, iron, manganese, and zinc) than MTA and BA. The setting time of BD was significantly shorter than that of MTA and BA, and the compressive strength of BA was significantly lower than that of MTA and BD. Conclusions: BA and BD were biocompatible, and they did not show any cytotoxic effects on human periodontal ligament fibroblasts. BA showed comparable cytotoxicity to MTA but inferior physical properties. BD had somewhat higher cytotoxicity but superior physical properties than MTA.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
Six specimens per test materials were placed individually in wells of sterile 48-well culture plate and then 1 mL DMEM with 10% fetal bovine serum and 1% antibiotics were added. The plates with the specimens were placed in the humidified incubator at 37℃ for 24 hours to obtain the extracts.
However, there are no reports describing the cytotoxicity of BDon hPDL fibroblasts compared to that of MTA and BA, or the possible effects of the release of heavy metals on cytotoxicity. The aim of this study is to assess the cytotoxicity of three tricalcium silicate-based endodontic materials (MTA, BA, and BD) by evaluating the cell viability of hPDL fibroblasts, to evaluate the concentration of9 heavy metals (arsenic, cadmium, chromium, copper, iron, lead, manganese, nickel, and zinc) leached out in distilled water, and finally, to examine the setting time and compressive strength of these three endodontic materials.
The hPDL fibroblasts and 100 μL of DMEM supplemented with 10% fetal bovine serum and 1% antibiotics were used as a positive control in addition to the BA, BD, and MTA groups. The cells exposed to the extracts were incubated for 1,3, and 7 days and XTT assay was performed at each time point.
After 7 days, the specimens were removed, and the solutions were filtered to remove the crystalline precipitate. The concentration of 9 heavy metals (arsenic, cadmium, chromium, copper, iron, lead, manganese, nickel, and zinc) in the extracts was examined using inductively coupled plasma-mass spectrometry (ICPMS, Perkin Elmer Nexion 300X, Perkin Elmer, Ontario, Canada). The measuring procedure was performed using ICP-MS with 4 repeats.
The concentration of 9 heavy metals (arsenic, cadmium, chromium, copper, iron, lead, manganese, nickel, and zinc) in the extracts was examined using inductively coupled plasma-mass spectrometry (ICPMS, Perkin Elmer Nexion 300X, Perkin Elmer, Ontario, Canada). The measuring procedure was performed using ICP-MS with 4 repeats. The significance of the difference in leachable heavy metal contents among BA, BD and MTA was evaluated.
All defective specimens were discarded, and 10 acceptable samples were prepared for each test material at each time interval. The specimens were immersed in distilled water for 24 hours, 3 days, and 7 days and maintained at 37℃. The compressive strengths were then measured by using a universal testing machine at a crosshead speed of 1.
28 The results of heavy metal analysis tend to vary widely.29Therefore, in this study the measuring procedure was performed using ICP-MS with 4 repeats, and the significance of differences in leachable heavy metal contents among BA, BD and MTA was evaluated. Copper, iron and manganese can cause discoloration of the teeth.
대상 데이터
, Woodland, CA, USA) and 1% antibiotics(Penicillin/Streptomycin, Gibco) in a humidified incubator(MCO-18AIC, Sanyo, Osaka, Japan) with 5% CO2 at 37℃. Human PDL fibroblasts from the third to eighth passages were used for this study.
The excess flash was removed. Six specimens of each material were fabricated. Samples were placed in a cabinet at 37℃ and relative humidity of 95 to 100%.
1 mm in diameter. The test materials were mixed and placed in a cylindrical stainless steel mold (10 mm in diameter and 5 mm in height). The assembly was placed in a cabinet at 37℃ and relative humidity of 95 to 100%, 2 minutes after the start of mixing.
Three materials were prepared: BA, BD, and MTA. The test materials were mixed according to the manufacturer’s instructions and placed into a sterile cylindrical polyethylene tube (5 mm in diameter and 3 mm in height).
To determine the amount of leachable heavy metals, we used ICP-MS to evaluate the release, and not the presence of 9 heavy metals (arsenic, cadmium, chromium, copper, iron, lead, manganese, nickel, and zinc) in the test materials. The overall concentration of heavy metals leached out in distilled water did not exceed 0.
데이터처리
Two-way analysis of variance (ANOVA) followed by Tukey post hoc test was used to determine statistically significant differences in cell viability and compressive strength according to the cultivation time and the test materials. One-way ANOVA followed by the Tukey post hoc test was used to test the differences in setting time and concentration of 9 heavy metals. A p-value < 0.
Two-way analysis of variance (ANOVA) followed by Tukey post hoc test was used to determine statistically significant differences in cell viability and compressive strength according to the cultivation time and the test materials. One-way ANOVA followed by the Tukey post hoc test was used to test the differences in setting time and concentration of 9 heavy metals.
성능/효과
In this study, although the cell viability of BD was significantly lower than that of MTA (p < 0.05) at 3 days, the cell viability of BA, BD and MTA was no significantly different compared to the DMEM control (p < 0.05), suggesting that all three materials do not have a cytotoxic effect on hPDL fibroblasts.
후속연구
It could be used as a root-end filling material. Further studies evaluating the in vivo biocompatibility and tooth discoloration caused by MTA, BA and BD are needed.
However, there is no prior report related to the discoloration of teeth after the use of BD. Further studies evaluating the tooth discoloration caused by MTA, BA and BD are needed.
참고문헌 (35)
1 Cohen S Hargreaves KM Chapter 21 Cohen's pathways of the pulp 10th ed St Louis Mosby Elsevier 2011
2 Johnson BR Considerations in the selection of a rootend filling material Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1999 87 398 404 10225620
3 Saxena P Gupta SK Newaskar V Biocompatibility of root-end filling materials: recent update Restor Dent Endod 2013 38 119 127 24010077
4 Torabinejad M Hong CU Pitt Ford TR Kettering JD Cytotoxicity of four root end filling materials J Endod 1995 21 489 492 8596067
5 Torabinejad M Higa RK McKendry DJ Pitt Ford TR Dye leakage of four root end filling materials: effects of blood contamination J Endod 1994 20 159 163 8035153
6 Koh ET Torabinejad M Pitt Ford TR Brady K McDonald F Mineral trioxide aggregate stimulates a biological response in human osteoblasts J Biomed Mater Res 1997 37 432 439 9368148
7 Torabinejad M Hong CU Lee SJ Monsef M Pitt Ford TR Investigation of mineral trioxide aggregate for rootend filling in dogs J Endod 1995 21 603 608 8596081
9 Monteiro Bramante C Demarchi AC de Moraes IG Bernadineli N Garcia RB Spångberg LS Duarte MA Presence of arsenic in different types of MTA and white and gray Portland cement Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008 106 909 913 18835535
10 Chang SW Shon WJ Lee W Kum KY Baek SH Bae KS Analysis of heavy metal contents in gray and white MTA and 2 kinds of Portland cement: a preliminary study Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010 109 642 646 20303061
11 Maroto M Barberia E Planells P Garcia Godoy F Dentin bridge formation after mineral trioxide aggregate (MTA) pulpotomies in primary teeth Am J Dent 2005 18 151 154 16158803
12 Boutsioukis C Noula G Lambrianidis T Ex vivo study of the efficiency of two techniques for the removal of mineral trioxide aggregate used as a root canal filling material J Endod 2008 34 1239 1242 18793929
13 Chang SW Chemical characteristics of mineral trioxide aggregate and its hydration reaction Restor Dent Endod 2012 37 188 193 23429542
14 Innovative BioCeramix, Inc.: Information updated 2012 Jan Available from: http://www.ibioceramix.com/Publications.html
15 Active Biosilicate Technology, Septodont. septodontusa.com updated 2013 Oct 10 Available from: http://www.septodontusa.com/products/biodentine
16 Park JW Hong SH Kim JH Lee SJ Shin SJ X-ray diffraction analysis of white ProRoot MTA and Diadent BioAggregate Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010 109 155 158 20123390
17 Zhang H Pappen FG Haapasalo M Dentin enhances the antibacterial effect of mineral trioxide aggregate and bioaggregate J Endod 2009 35 221 224 19166777
18 De-Deus G Canabarro A Alves G Linhares A Senne MI Granjeiro JM Optimal cytocompatibility of a bioceramic nanoparticulate cement in primary human mesenchymal cells J Endod 2009 35 1387 1390 19801236
19 Yuan Z Peng B Jiang H Bian Z Yan P Effect of bioaggregate on mineral-associated gene expression in osteoblast cells J Endod 2010 36 1145 1148 20630287
20 Chung CR Kim E Shin SJ Biocompatibility of bioaggregate cement on human pulp and periodontal ligament (PDL) derived cells J Korean Acad Conserv Dent 2010 35 473 478
21 Yan P Yuan Z Jiang H Peng B Bian Z Effect of bioaggregate on differentiation of human periodontal ligament fibroblasts Int Endod J 2010 43 1116 1121 20812946
22 Grech L Mallia B Camilleri J Investigation of the physical properties of tricalcium silicate cement-based root-end filling materials Dent Mater 2013 29 e20 e28 23199808
23 Laurent P Camps J De Méo M Déjou J About I Induction of specific cell responses to a Ca(3)SiO(5)-based posterior restorative material Dent Mater 2008 24 1486 1494 18448160
24 Laurent P Camps J About I Biodentine(TM) induces TGF-β1 release from human pulp cells and early dental pulp mineralization Int Endod J 2012 45 439 448 22188368
25 Peng W Liu W Zhai W Jiang L Li L Chang J Zhu Y Effect of tricalcium silicate on the proliferation and odontogenic differentiation of human dental pulp cells J Endod 2011 37 1240 1246 21846540
28 Camilleri J Kralj P Veber M Sinagra E Characterization and analyses of acid-extractable and leached trace elements in dental cements Int Endod J 2012 45 737 743 22394277
29 Chang SW Baek SH Yang HC Seo DG Hong ST Han SH Lee Y Gu Y Kwon HB Lee W Bae KS Kum KY Heavy metal analysis of ortho MTA and ProRoot MTA J Endod 2011 37 1673 1676 22099903
33 Huan Z Chang J Study on physicochemical properties and in vitro bioactivity of tricalcium silicate-calcium carbonate composite bone cement J Mater Sci Mater Med 2008 19 2913 2918 18347949
34 Lee BN Hwang YC Jang JH Chang HS Hwang IN Yang SY Park YJ Son HH Oh WM Improvement of the properties of mineral trioxide aggregate by mixing with hydration accelerators J Endod 2011 37 1433 1436 21924197
※ AI-Helper는 부적절한 답변을 할 수 있습니다.