[논문]Tyrosinase 및 TRP-2의 발현 억제를 통한 황칠나무 잎 추출물의 Melanin 생성 저해 효과

박수아; 이혜미; 하지훈; 전소하; 박수남

doi:10.14478/ace.2014.1058

[국내논문] Tyrosinase 및 TRP-2의 발현 억제를 통한 황칠나무 잎 추출물의 Melanin 생성 저해 효과
Inhibitory Effects of Dendropanax Morbifera Leaf Extracts on Melanogenesis through Down-Regulation of Tyrosinase and TRP-2 원문보기

공업화학 = Applied chemistry for engineering, v.25 no.5, 2014년, pp.468 - 473

박수아 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실, 화장품종합기술연구소) , 이혜미 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실, 화장품종합기술연구소) , 하지훈 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실, 화장품종합기술연구소) , 전소하 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실, 화장품종합기술연구소) , 박수남 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실, 화장품종합기술연구소)

초록
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황칠나무는 한국의 남부 해안 및 제주도 등에서 자생하는 식물이다. 본 연구에서는 황칠나무 잎 추출물의 미백 활성의 원인을 규명하고자 연구를 수행하였다. ${\alpha}$-MSH로 자극된 B16 melanoma 세포에 대한 추출물의 멜라닌 생합성 저해 활성을 측정하였다. 추출물은 25 및 $50{\mu}g/mL$ 농도에서 유의적으로 멜라닌 함량을 감소시켰으며, 세포 내의 tyrosinase 활성뿐만 아니라 tyrosinase와 TRP-2의 단백질 발현도 상당히 저해하였다. 결과적으로, 황칠나무 잎 추출물은 세포 내의 tyrosinase활성과 멜라닌 생합성에 직접적으로 관련된 효소의 발현을 저해함으로써 미백효과를 나타내었다. 본 연구 결과는 황칠나무 잎 추출물이 새로운 미백 화장품의 원료로써 이용 가능함을 시사한다.

Abstract ▼ AI-Helper

Dendropanax morbifera (D. morbifera) grows in the southern coastal areas and on Jeju Island in Korea. In this study, D. morbifera leaf extract was investigated to determine the mechanism of its whitening effect. The inhibitory activities of the extract on melanogenesis were tested in B16 melanoma cells treated with the ${\alpha}$-melanocyte stimulating hormone (${\alpha}$-MSH). D. morbifera leaf extracts remarkably decreased the melanin content at 25 and $50{\mu}g/mL$. The extracts significantly inhibited the intracellular tyrosinase activity and protein expression of tyrosinase and tyrosinase related protein-2 (TRP-2). In conclusion, D. morbifera leaf extracts would show a whitening effect by inhibiting intracellular tyrosinase activities and the expression of enzymes directly involved in the melanin biosynthesis. The results indicate that fractions of D. morbifera leaf extracts show potential for application as a whitening agent in the new whitening cosmetics.

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문제 정의

In this study, we aimed to reveal the mechanism underlying the ability of D. morbifera leaf extracts to inhibit melanin biosynthesis and expression of melanin synthesis related proteins using B16 melanoma cells. We propose that D.

제안 방법

The inhibitory effects of D. morbifera leaf extract on tyrosinase activity in cells were examined by measuring the intracellular tyrosinase activity. B16 melanoma cells were seeded on a 6-well plate template at a density of 1 × 10⁵cells/well at 37 ℃ in a 5% CO₂ atmosphere.
morbifera leaf extracts on intracellular tyrosinase activity in B16 melanoma cells. After treatment with various concentrations of the ethyl acetate fraction (A; DM EA) and the aglycone fraction (B; DM Agly) of D. morbifera leaf extracts for 3 days, their tyrosinase inhibitory activities were investigated in the cellular level.
The experiment was carried out in 90 µL of reaction mixture comprising the cell extract (40 µg), 1 M sodium phosphate buffer (pH 6.8, Biosesang Inc.)with various concentrations of D. morbifera leaf extract (3.125 ~ 50 µg/mL), and then 10 µL L-DOPA was added.
The experiment was carried out in 90 µL of reaction mixture comprising the cell lysates as the tyrosinase enzyme source (40 µg protein), 1 M sodium phosphate buffer (pH 6.8, Biosesang Inc.), and 10 µL L-DOPA.

대상 데이터

(Pasching, Austria). Air-dried D. morbifera leaves were purchased from the Jecheon medicinal plant market in June 2012 (Hwasan-dong, Jecheon-si, Chungbuk, South Korea).

데이터처리

All reported data are presented as mean ± S.E.M. Statistical significance was determined using the Student’s t-test.

이론/모형

Cell viability was determined using an MTT assay. B16 melanoma cells were seeded on a 96-well plate template at 37 ℃ in a 5% CO₂ atmosphere.

성능/효과

The ethyl acetate fraction of D. morbifera leaf extracts administered at 6.25, 12.5, 25, and 50 µg/mL showed 2%, 13%, 26%, and 35% intracellular tyrosinase inhibitory activity, respectively, compared with the group treated with α-MSH alone (+ 85% of control).
The results showed that D. morbifera leaf extracts do not directly inhibit tyrosinase activity. However, the extracts showed an inhibitory effect on melanogenesis, and therefore must affect a process either before or after the tyrosinase step in B16 melanoma cells.
These results indicate that fractions of D. morbifera leaf extract show potential for application as a whitening agent in development of the new whitening cosmetics via the inhibition of α-MSH-induced melanin biosynthesis.

참고문헌 (25)

H. Ando, Y. Niki, M. Ito, K. Akiyama, M. S. Matsui, D. B. Yarosh, and M. I. Chihashi, Melanosomes are transferred from melanocytes to keratinocytes through the processes of packaging, release, uptake, and dispersion, J. Invest. Dermatol., 132, 1222-1229 (2012).

상세보기
M. Seiberg, Keratinocyte-melanocyte interation during melanosome transfer, Pigment. Cell Res., 14, 236-242 (2001).

상세보기
Y. Yamaguchi, M. Brenner, and V. J. Hearing, The regulation of skin pigmentation, J. Biol. Chem., 282, 27557-27561 (2007).

상세보기
G. E. Costin and V. J. Hearing, Human skin pigmentation: melanocytes modulate skin color in response to stress, FASEB J., 21, 976-994 (2007).

상세보기
H. Li, J. Kim, H. G. Hahn, J. Yun, H. S. Jeong, H. Y. Yun, K. J. Baek, N. S. Kwon, Y. S. Min, K. C. Park, and D. S. Kim, KHG26792 inhibits melanin synthesis in mel-Ab cells and a skin equivalent model, Korean J. Physiol. Pharmacol., 18, 249-254 (2014).

원문보기 상세보기
R. Busca and R. Ballotti, Cyclic AMP a key messenger in the regulation of skin pigmentation, Pigment Cell Res., 13, 60-69 (2000).

상세보기
M. Iwata, T. Corn, S. Iwata, M. A. Everett, and B. B. Fuller, The relationship between tyrosinase activity and skin color in human foreskins, J. Invest. Dermatol., 95, 9-15 (1990).

상세보기
K. Kameyama, T. Takemura, Y. Hamada, C. Sakai, S. Kondoh, S. Nishiyama, K. Urabe, and V. J. Hearing, Pigment production in murine melanoma cells is regulated by tyrosinase, tyrosinase-related protein 1 (TRP1), DOPA chrometautomerase (TRP2), and a melanogenic inhibitor, J. Invest. Dermatol., 100, 126-131 (1993).

상세보기
E. J. Seo, E. S. Hong, M. H. Choi, K. S. Kim, and S. J. Lee, Antioxidant and skin whitening effects of Rhamnus yoshinoi extracts, Korean J. Food Scl. Technol., 42, 750-754 (2010).
H. Seo, G. Y. Seo, S. Z. Ko, and Y. H. Park, Inhibitory effects of ethanol extracts from Polygonimultiflori radix and Cynanchiwilfordii radix on melanogenesis in melanoma cells. J. Korean Soc. Food Sci. Nutr., 40, 1086-1091 (2011).

원문보기 상세보기
L. Weixiong and Z. H. Helene, Induced melanin reduces mutations and cell killing in mouse melanoma, Phytochem. Phytobiol., 65, 480-485 (1997).

상세보기
N. R. Jo, M. A. Park, K. Y. Chae, S. A. Park, S. H. Jeon, J. H. Ha, and S. N. Park, Cellular protective and antioxidative acivities of Parthenocissus tricuspidata stem extracts, J. Soc. Cosmet. Scientists Korea, 38, 225-236 (2012).

원문보기 상세보기
L. Packer, Ultraviolet radiation (UVA, UVB) and skin antioxidants. In: C. A. Rice-Evans, and R. H. Burdon (eds.). Free radical damage and its control. Elsevier Science, Amsterdam (1994).
K. Scharffetter-Kochanek, Photoaging of the connective tissues of skin: Its prevention and therapy, Adv. Pharmacol., 38, 639-655 (1997).
K. H. Bae, J. A. Kim, and Y. E. Choi, Induction and in vitro proliferation of adventitious roots in Dendropanax morbifera, J. Plant Biotechnol., 36, 163-169 (2009).

원문보기 상세보기
S. M. An, J. S. Koh, and Y. C. Boo, p-coumaric acid not only inhibits human tyrosinase activity in vitro but also melanogenesis in cells exposed to UVB, Phytotherapy Research, 24, 1175-1180 (2010).

상세보기
S. M. An, S. I. Lee, S. W. Choi, S. W. Moon, and Y. C. Boo, p-Coumaric acid, a constituent of Sasa quelpaertensis Nakai, inhibits cellular melanogenesis stimulated by ${\alpha}$ -melanocyte stimulating hormone, British J. Dermatol., 159, 292-299 (2008).

상세보기
M. K. Huh, M. H. Han, C. Park, and Y. H. Choi, Inhibitory effects of Phyllostachys bambusoides on melanin synthesis and tyrosinase activity in cultured human melanoma cells, J. Life Sci., 24, 284-289 (2014).

원문보기 상세보기
H. Y. Park, R. Vladimir, O. Shigeo, and B. A. Gilchrest, The ${\beta}$ Isoform of protein kinase C stimulates human melanogenesis by activating tyrosinase in pigment cells, J. Boil. Chem., 268, 11742-11749 (1993).
S. Kazuomi, T. Hideki, I. Remina, and T, Masaru, Down-regulation of tyrosinase expression by acetylsalicylic acid in murine B16 melanoma, Biol. Pharm. Bull., 31, 33-37 (2008).

상세보기
M. Kim, J. Park, K. Song, H. G. Kim, J. S. Koh, and Y. C. Boo, Screening of plant extracts for human tyrosinase inhibiting effects, Int. J. Cos. Sci., 34, 202-208 (2012).

상세보기
D. S. Kim, S. Y. Kim, S. H. Park, Y. G. Choi, S. B. Kwon, M. K. Kim, J. I. NA, S. W. Youn, and K. C. Park, Inhibitory effects of 4-n-butylresorcinol on tyrosinase activity and melanin synthesis, Biol. Pharm. Bull., 28, 2216-2219 (2005).

상세보기
K. Maeda and M. Fukuda, Arbutin: mechanism of its depigmenting action in human melanocyte culture. J. Phamacol. Exp. Ther., 276, 765-769 (1996).
G. Battaini, E. Monzani, L. Casella, L. Santagostini, and R. Pagliarin, Inhibition of the catecholase activity of biomimetic dinuclear copper complexes by kojic acid. J. Biol. Inorg. Chem., 5, 262-268 (2000).

상세보기
A. Tuerxuntayi, Y. Q. Liu, A. Tulake, M. Kabas, A. Eblimit, and H. A. Aisa, Kaliziri extract upregulates tyrosinase, TRP-1, TRP-2 and MITF expression in murine B16 melanoma cells, BMC Complement. Altern. Med., 14, 166 (2014).

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