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The Bioconversion of Red Ginseng Ethanol Extract into Compound K by Saccharomyces cerevisiae HJ-014 원문보기

Mycobiology, v.42 no.3, 2014년, pp.256 - 261  

Choi, Hak Joo (Traditional and Biomedical Research Center, Daejeon University) ,  Kim, Eun A (Traditional and Biomedical Research Center, Daejeon University) ,  Kim, Dong Hee (Traditional and Biomedical Research Center, Daejeon University) ,  Shin, Kwang-Soo (Division of Life Sciences, College of Natural Science, Daejeon University)

Abstract ▼ AI-Helper 아이콘AI-Helper

A ${\beta}$-glucosidase producing yeast strain was isolated from Korean traditional rice wine. Based on the sequence of the YCL008c gene and analysis of the fatty acid composition, the isolate was identified as Saccharomyces cerevisiae strain HJ-014. S. cerevisiae HJ-014 produced ginsenos...

주제어

#Bioconversion   #Compound K   #Red ginseng extract   #Saccharomyces cerevisiae HJ-014  

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제안 방법

  • PCR amplification was performed using Saccharomyces specific primers YCL008c-U (5'-TTCCGTTGGATGTGCCATCG-3') and YCL008c-L (5'-GGAGCCACCAAGGGATGG-3') [20].
  • 1B). The consensus sequence of the Saccharomyces YCL008c gene (1,514 bp) from the isolate was aligned with other S. cerevisiae sequences and the taxonomic relationships were analyzed. Phylogenetic trees based on the aligned sequences were constructed using MEGA ver.

이론/모형

  • cerevisiae sequences and the taxonomic relationships were analyzed. Phylogenetic trees based on the aligned sequences were constructed using MEGA ver. 5.1 [22] with neighbor-joining [23], based on 1,000 randomly chosen bootstrap replication and tree/branch style was circle (Fig. 1C). The sequence was 97% homologous to the S.
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참고문헌 (28)

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    상세보기

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