선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- One of the most interesting ways to use immobilized enzymes is through their ability to be recovered and used multiple times [14]. In this study, the recovery ability of immobilized K80 was evaluated by repeating the pNPC assay 10 times. The immobilized K80 activity decreased gradually but retained >80% of initial activity after five reactions and about 42% after 10 reactions (Fig.
대상 데이터
- p-Nitrophenyl esters, tributyrin, tricaprylin, fish oil, coconut oil, olive oil, and soybean oil were purchased from Sigma-Aldrich (St. Louis, MO, USA). Castor oil was acquired from the OCI Co.
이론/모형
- The homology model of lipase K80 (Fig. 3A) was constructed through the SWISS-MODEL homology modeling server using the P. mirabilis structure as the template. P.
- 1) was submitted to the SWISS-MODEL homology modeling server [1, 2], and modeling was performed in the automatic mode. The homology model was based on the crystal structure (PDB code: 4GW3) of P. mirabilis lipase with 80% amino acid identity [9].
- aHydrolytic activity of soluble lipase K80 in the supernatant was measured using pNPC method.
- bHydrolytic activity of immobilized lipase K80 on beads was measured using pNPC method.
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