Different with other fishes, the guppies (Poecilia reticulata) is ovoviviparity, which retain their fertilized eggs within the follicle throughout gestation. The synchronously growing diplotene oocytes store nutrients in droplets and yolk, before their maturation and fertilization. The lecithotrophi...
Different with other fishes, the guppies (Poecilia reticulata) is ovoviviparity, which retain their fertilized eggs within the follicle throughout gestation. The synchronously growing diplotene oocytes store nutrients in droplets and yolk, before their maturation and fertilization. The lecithotrophic strategy of development entails the provisioning of embryos with resources from the maternal yolk deposit rather than from a placenta, it allows the extracorporeal culture of guppy embryo. Studies on their early development of live bearers like the guppy including lineage tracing and genetic manipulations, have been limited. Therefore, to optimize conditions of embryo in vitro culture, explanted embryos from pregnant females were incubated in embryo medium (L-15 medium, supplemented with 5, 10, 15, 20% fetal bovine serum, respectively). We investigated whether the contents of FBS in vitro culture medium impact the development of embryos, and whether they would hatch in vitro. Our study found that in 5% of FBS of the medium, although embryos developed significantly slower in vitro than in the ovary, it was impossible to exactly quantify the developmental delay in culture, due to the obvious spread in developmental stage within each batch of eggs, and embryos can only be maintained until the early-eyed. And although in culture with 20% FBS the embryos can sustain rapid development of early stage, but cannot be cultured for the entire period of their embryonic development and ultimately died. In the medium with 10% and 15% FBS, the embryos seems well developed, even some can continue to grow after follicle ruptures until it can be fed. We also observed that embryonic in these two culture conditions were significantly different in development speed, in 15% it is faster than 10%. But 10% FBS appears to be more optimizing condition than 15% one on development process of embryos and survival rate to larvae stage.
Different with other fishes, the guppies (Poecilia reticulata) is ovoviviparity, which retain their fertilized eggs within the follicle throughout gestation. The synchronously growing diplotene oocytes store nutrients in droplets and yolk, before their maturation and fertilization. The lecithotrophic strategy of development entails the provisioning of embryos with resources from the maternal yolk deposit rather than from a placenta, it allows the extracorporeal culture of guppy embryo. Studies on their early development of live bearers like the guppy including lineage tracing and genetic manipulations, have been limited. Therefore, to optimize conditions of embryo in vitro culture, explanted embryos from pregnant females were incubated in embryo medium (L-15 medium, supplemented with 5, 10, 15, 20% fetal bovine serum, respectively). We investigated whether the contents of FBS in vitro culture medium impact the development of embryos, and whether they would hatch in vitro. Our study found that in 5% of FBS of the medium, although embryos developed significantly slower in vitro than in the ovary, it was impossible to exactly quantify the developmental delay in culture, due to the obvious spread in developmental stage within each batch of eggs, and embryos can only be maintained until the early-eyed. And although in culture with 20% FBS the embryos can sustain rapid development of early stage, but cannot be cultured for the entire period of their embryonic development and ultimately died. In the medium with 10% and 15% FBS, the embryos seems well developed, even some can continue to grow after follicle ruptures until it can be fed. We also observed that embryonic in these two culture conditions were significantly different in development speed, in 15% it is faster than 10%. But 10% FBS appears to be more optimizing condition than 15% one on development process of embryos and survival rate to larvae stage.
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제안 방법
Remaining follicles detached from the embryos within the first 24 hr of culture and were removed. Embryos were inspected daily and medium was changed twice a week.
성능/효과
(2006) cultured guppy embryos with 15% L-15 medium, and can continue development. However, our results according to experimental data showed, both 10% and 15% FBS in medium can be used for in vitro culture, but the development in 10% FBS appears to be more optimizing condition than 15% FBS on development process of embryos and survival rate to larvae stage (Table 1).
참고문헌 (15)
1 Endler JA Variation in the appearance of guppy color patterns to guppies and their predators under different visual conditions Vision Res 1991 31 587 608 1843763
2 Endler JA Multiple-trait coevolution and environmental gradients in guppies Trends Ecol Evol 1995 10 22 29 21236940
3 Evans JP Zane L Francescato S Pilastro A Directional postcopulatory sexual selection revealed by artificial insemination Nature 2003 421 360 363 12540898
4 Haas-Andela H In vitro culture of embryos of the Xiphophorine fish and their raising to adulthood Zool Anz Jena 1976 197 1 5
5 Lambert JGD The ovary of the guppy Poecilia reticulata. The granulosa cells as sites of steroid biogenesis Gen Comp Endocrinol 1970 15 464 476 5500428
6 Magurran AE Henderson PA Explaining the excess of rare species in natural species abundance distributions Nature 2003 422 714 716 12700760
8 Reznick DN Mateos M Springer MS Independent origins and rapid evolution of the placenta in the fish genus Poeciliopsis Science 2002 298 1018 1020 12411703
9 Reznick DN Life history evolution in guppies ( Poecilia reticulata ): guppies as a model for studying the evolutionary biology of aging Exp Gerontol 1997 32 245 258 9193894
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