Bixofis, Regiane B.
(Department of Oral and Maxillofacial Surgery, Erasto Gaertner Hospital)
,
Sassi, Laurindo Moacir
(Department of Oral and Maxillofacial Surgery, Erasto Gaertner Hospital)
,
Patussi, Cleverson
(Department of Oral and Maxillofacial Surgery, Erasto Gaertner Hospital)
,
Jung, Juliana E.
(Department of Pathology, Erasto Gaertner Hospital)
,
Ioshii, Sergio O.
(Department of Pathology, Erasto Gaertner Hospital)
,
Schussel, Juliana L.
(Department of Oral and Maxillofacial Surgery, Erasto Gaertner Hospital)
Background: The involvement of HPV in oral and oropharyngeal carcinogenesis was first proposed in 2004, based on epithelial HPV tropism and detection of HPV genotypes in oral squamous cell carcinoma samples. While 60-70% of oropharynx tumors may be HPV-positive, only 10 to 19% of tumors of the oral ...
Background: The involvement of HPV in oral and oropharyngeal carcinogenesis was first proposed in 2004, based on epithelial HPV tropism and detection of HPV genotypes in oral squamous cell carcinoma samples. While 60-70% of oropharynx tumors may be HPV-positive, only 10 to 19% of tumors of the oral cavity, larynx and hypopharynx appear to have HPV infection. The aim of the study was to evaluate HPV infection associated with oropharyngeal cancer. Materials and Methods: Seventy-eight cases were selected for p16 immunoexpression reactions, and demographic data were collected for comparisons. Results: Most patients were over 60 years old, and 64.1% were smokers. Immunohistochemistry results showed that 86.3% of cases stained positive for p16 protein. Conclusion: The oropharyngeal cancer profile at Erasto Gaertner Hospital presented a high index of smokers over 60 years as well a high number of p16+ tumors, for what we can not determinate the main etiologic factor, but can be aware of the number of patients that presented HPV infection. Since prevention is still the best way to deal with cancer disease, it is important to analyze the interaction of these two etiologic factors and how to detect lesions at an early stage.
Background: The involvement of HPV in oral and oropharyngeal carcinogenesis was first proposed in 2004, based on epithelial HPV tropism and detection of HPV genotypes in oral squamous cell carcinoma samples. While 60-70% of oropharynx tumors may be HPV-positive, only 10 to 19% of tumors of the oral cavity, larynx and hypopharynx appear to have HPV infection. The aim of the study was to evaluate HPV infection associated with oropharyngeal cancer. Materials and Methods: Seventy-eight cases were selected for p16 immunoexpression reactions, and demographic data were collected for comparisons. Results: Most patients were over 60 years old, and 64.1% were smokers. Immunohistochemistry results showed that 86.3% of cases stained positive for p16 protein. Conclusion: The oropharyngeal cancer profile at Erasto Gaertner Hospital presented a high index of smokers over 60 years as well a high number of p16+ tumors, for what we can not determinate the main etiologic factor, but can be aware of the number of patients that presented HPV infection. Since prevention is still the best way to deal with cancer disease, it is important to analyze the interaction of these two etiologic factors and how to detect lesions at an early stage.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
After primary antibody exposure, the slides were washed and treated with biotinylated antibody for 30 min. Antigen visualization was achieved using ENVISION FLEX (DAKO, Carpinteria, CA, USA) for 30 min followed by diaminobenzidine chromogen (DAKO Liquid DAB+, K3468). Slides were counter-stained with haematoxylin.
Medical records were consulted to collect data such as age, sex, occupation, diagnosis, time of evolution, family history, treatment, risk factors and prognosis.
The aim of the study was to evaluate the prevalence of HPV infection associated with oropharyngeal cancers at Erasto Gaertner Hospital and assess patient’s risk factors profile.
대상 데이터
Considering patients under 50 years old, 70% showed p16 positive expression and from that 35% are alive, free of the disease. All of these patients were smokers.
Patients with squamous cell carcinoma of the oropharynx (SCCO) treated at Erasto Gaertner Hospital between the years 2005 to 2009 were selected.
Seventy-eight patients were selected for these study, 71 male and 7 female, most of them leucoderma (73%). Fifty percent of patients were in the 6℃ decade of life.
데이터처리
The results were analyzed using chi-square test. For all tests, p value was <0.
성능/효과
After treatment, 6.4% of patients were free of the disease, 19.2% presented residual disease or metastasis, 8.9% had second primary tumor, 6.4% presented a tumor recidive and 50% did not finish treatment for unknown reasons. Follow-up of these patients showed that 16.
후속연구
Although we cannot state that our cohort was composed of HPV-related tumor, it showed a high prevalence of HPV, showing that the virus is present in great part of patients. More studies must be done to investigate the interaction of tobacco and HPV on oral carcinogenesis.
참고문헌 (19)
Chaturvedi AK, Engels EA, Anderson WF, et al (2008). Incidence trends for human papillomavirus-related and-unrelated oral squamous cell carcinomas in the United States. J Clin Oncol, 26, 612-9.
El-Naggar AK, Westra WH (2012). p16 expression as a surrogate marker for HPV-related oropharyngeal carcinoma: a guide for interpretative relevance and consistency. Head Neck, 34, 459-61.
Elango KJ, Suresh A, Erode EM, et al (2011). Role of human papilloma virus in oral tongue squamous cell carcinoma. Asian Pac J Cancer Prev, 12, 889-96.
Gillison ML, Shah KV (2001). Human papillomavirus-associated head and neck squamous cell carcinoma: mounting evidence for an etiologic role for human papillomavirus in a subset of head and neck cancers. Curr Opin Oncol, 13, 183-8.
Hammarstedt L, Lindquist D, Dahlstrand H, et al (2006). Human papillomavirus as a risk factor for the increase in incidence of tonsillar cancer. Int J Cancer, 119, 2620-3.
Huang H, Zhang B, Chen W, et al (2012). Human papillomavirus infection and prognostic predictors in patients with oropharyngeal squamous cell carcinoma. Asian Pac J Cancer Prev, 13, 891-6.
Jalilvand S, Shoja Z, Hamkar R (2014). Human papillomavirus burden in different cancers in Iran: a systematic assessment. Asian Pac J Cancer Prev, 15, 7029-35.
Kong CS, Balzer BL, Troxell ML, et al (2007). p16INK4A immunohistochemistry is superior to HPV in situ hybridization for the detection of high-risk HPV in atypical squamous metaplasia. Am J Surg Pathol, 31, 33-43.
Kreimer AR, Clifford GM, Boyle P, et al (2005). Human papillomavirus types in head and neck squamous cell carcinomas worldwide: a systematic review. Cancer Epidemiol Biomarkers Prev, 14, 467-75.
Munoz N, Bosch FX, De Sanjose S, et al (2003). Epidemiologic classification of human papillomavirus types associated with cervical cancer. N Engl J Med, 348, 518-27.
Pannone G, Santoro A, Papagerakis S, et al (2011). The role of human papillomavirus in the pathogenesis of head & neck squamous cell carcinoma: an overview. Infect Agent Cancer, 6, 4.
Ragin CC, Modugno F, Gollin SM (2007). The epidemiology and risk factors of head and neck cancer: a focus on human papillomavirus. J Dent Res, 86, 104-14.
Syrjanen S, Shabalova I, Petrovichev N, et al (2004). Acquisition of high-risk human papillomavirus infections and pap smear abnormalities among women in the new independent states of the former soviet union. J Clin Microbiol, 42, 505-11.
Tural D, Elicin O, Batur S, et al (2013). Increase in the rate of HPV positive oropharyngeal cancers during 1996-2011 in a case study in Turkey. Asian Pac J Cancer Prev, 14, 6065-8.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.