[논문]Allelic Diversity of MSP1 Gene in Plasmodium falciparum from Rural and Urban Areas of Gabon

Mawili-Mboumba, Denise Patricia; Mbondoukwe, Noe; Adande, Elvire; Bouyou-Akotet, Marielle Karine

doi:10.3347/kjp.2015.53.4.413

Allelic Diversity of MSP1 Gene in Plasmodium falciparum from Rural and Urban Areas of Gabon 원문보기

The Korean journal of parasitology, v.53 no.4, 2015년, pp.413 - 419

Mawili-Mboumba, Denise Patricia (Department of Parasitology-Mycology, Faculty of Medicine, Universite des Sciences de la Sante) , Mbondoukwe, Noe (Department of Parasitology-Mycology, Faculty of Medicine, Universite des Sciences de la Sante) , Adande, Elvire (Department of Parasitology-Mycology, Faculty of Medicine, Universite des Sciences de la Sante) , Bouyou-Akotet, Marielle Karine (Department of Parasitology-Mycology, Faculty of Medicine, Universite des Sciences de la Sante)

Abstract ▼ AI-Helper

The present study determined and compared the genetic diversity of Plasmodium falciparum strains infecting children living in 2 areas from Gabon with different malaria endemicity. Blood samples were collected from febrile children from 2008 to 2009 in 2 health centres from rural (Oyem) and urban (Owendo) areas. Genetic diversity was determined in P. falciparum isolates by analyzing the merozoite surface protein-1 (msp1) gene polymorphism using nested-PCR. Overall, 168 children with mild falciparum malaria were included. K1, Ro33, and Mad20 alleles were found in 110 (65.5%), 94 (55.9%), and 35 (20.8%) isolates, respectively, without difference according to the site (P>0.05). Allelic families' frequencies were comparable between children less than 5 years old from the 2 sites; while among the older children the proportions of Ro33 and Mad20 alleles were 1.7 to 2.0 fold higher at Oyem. Thirty-three different alleles were detected, 16 (48.5%) were common to both sites, and 10 out of the 17 specific alleles were found at Oyem. Furthermore, multiple infection carriers were frequent at Oyem (57.7% vs 42.2% at Owendo; P=0.04) where the complexity of infection was of 1.88 (${\pm}0.95$) higher compared to that found at Owendo ($1.55{\pm}0.75$). Extended genetic diversity of P. falciparum strains infecting Gabonese symptomatic children and high multiplicity of infections were observed in rural area. Alleles common to the 2 sites were frequent; the site-specific alleles predominated in the rural area. Such distribution of the alleles should be taken into accounts when designing MSP1 or MSP2 malaria vaccine.

주제어

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문제 정의

This study was a part of a health facility-based project initiated by the Malaria National Control Program (MNCP), which aimed to evaluate malaria prevalence and to perform molecular analysis of P. falciparum isolates. Throughout the study, children under 11 years old, presenting at hospital for consultation with fever (axillary temperature ≥37.

제안 방법

The main limitation of this study is the use of a single marker that has most likely reduced the genetic diversity evaluation of the parasites strains. Nonetheless, previous studies showed an extended genetic diversity of P.

대상 데이터

Thirty-three different alleles were detected; 14 K1-type, 14 Mad20-type, and 5 Ro33-type alleles (Fig. 1). A half of these alleles (n=16; 48.

데이터처리

Differences between groups were assessed using the chi-square or Fisher’s exact tests for proportions.

이론/모형

Continuous variables are presented as medians 25th and 75th percentiles, and were compared using the student’s t-test, Mann Whitney U-test or Kruskal-Wallis test as appropriate.
DNA was extracted from the filter paper using the methanol extraction method [16]. The block 2 of msp1 gene was amplified by primary PCR followed by nested rounds of PCR using allele specific primers.

성능/효과

No specific Ro33 allele was detected in the urban city of Owendo. MSP1 allelic diversity and age In each study site, most of K1, Mad20, and Ro33 alleles were detected in isolates from children aged less than 5 years old; notably Mad20 site specific alleles (Table 1).
falciparum strains. Thus, the present study examined and compared the genetic diversity of P. falciparum isolates from children living in 1 urban and 1 rural areas of Gabon using a highly polymorphic gene encoding MSP1.

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