[논문]α-Glucosidase Inhibition Activity of the Extracts of Katsura Tree (Cercidiphyllum japonicum Sieb. Et Zucc) Leaves

Lee, Tae-Seong; Ryu, Wang-Gyun; Bae, Young-Soo

doi:10.5658/wood.2015.43.2.238

α-Glucosidase Inhibition Activity of the Extracts of Katsura Tree (Cercidiphyllum japonicum Sieb. Et Zucc) Leaves 원문보기

목재공학 = Journal of the Korean wood science and technology, v.43 no.2, 2015년, pp.238 - 247

Lee, Tae-Seong (Samcheok City Agricultural Technology and Extension Center) , Ryu, Wang-Gyun (Samcheok City Agricultural Technology and Extension Center) , Bae, Young-Soo (Department of Forest Biomaterials Engineering, College of Forest and Environment Sciences, Kangwon National University)

Abstract ▼ AI-Helper

Katsura tree (Cercidiphyllum japonicum Sieb. Et Zucc) leaves were collected, air-dried and extracted with 70% aqueous acetone, then concentrated and sequentially fractionated using n-hexane, $CH_2Cl_2$, EtOAc, and $H_2O$ to be freeze dried for antioxidant and ${\alpha}$-glucosidase inhibition activity tests. The antioxidant activity of the extracts was evaluated using DPPH (1,1-diphenyl 2-picrylhydrazyl) free radical scavenging assay. The test concentrations were adjusted to 500, 250, 125, 62.5, 31.25, 15.62, 7.81, 3.9, 1.95 and 0.97 ppm. The $H_2O$ and EtOAc fractions showed higher activities compared with the control, ${\alpha}$-tocopherol, at all concentrations. The crude fraction also gave better activity at the concentrations lower than 62.5 ppm. However, the nonpolar n-hexane and $CH_2Cl_2$ fractions gave prominently lower activities compared with the control at all concentrations. The $IC_{50}$ values of the crude, EtOAc, and $H_2O$ fractions exhibited 11.78, 4.29 and $9.80{\mu}g/m{\ell}$, respectively, compared with $12.08{\mu}g/m{\ell}$ of the control. But the n-hexane and $CH_2Cl_2$ fractions indicated 300 and $91.85{\mu}g/m{\ell}$ of $IC_{50}$, respectively. ${\alpha}$-Glucosidase inhibition activity was evaluated at the concentrations of 50, 25, 12.5, 6.3, 3.1, 1.6 and 0.8 ppm. The inhibition activities were increased according to as the increase of sample concentrations. However, the nonpolar n-hexane and $CH_2Cl_2$ fractions indicated very low inhibition activities compared with acarbose, a positive control. The EtOAc fraction showed very good capability as almost 100% compared with the control at the higher concentrations than 12.5 ppm and the crude fraction also indicated good potential as 95% and 100% at 25 and 50 ppm, respectively. The $H_2O$ fraction gave good inhibition value as 90% at 50 ppm although the value was lower than the control. These results showed that the polar fractions had better ${\alpha}$-glucosidase inhibition activities. The $IC_{50}$ values of the nonpolar fractions, n-hexane and $CH_2Cl_2$, showed very lower values as 468 and $103.6{\mu}g/m{\ell}$, respectively, than the control. ${\alpha}$-Glucosidase Inhibition Activity of the Extracts of Katsura Tree (Cercidiphyllum japonicum Sieb. Et Zucc) Leaves However, the polar fractions, crude, EtOAc and $H_2O$, showed 7.1, 3.7 and $13{\mu}g/m{\ell}$, respectively, indicating that these fractions can be used as natural bioresources for treating diabetes mellitus. Also ${\alpha}$-glucosidase inhibition activity had a positive correlation with antioxidant activity of the extracts.

주제어

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문제 정의

This work was to evaluate antioxidant and antidiabetic activity of the extracts of katsura tree leaves for future functional and pharmaceutical use of the species.

이론/모형

Then the supernatant was dialyzed for 24 hr. Protein was determined by the method of Bradford (Schmidt et al. 1979).
The antioxidant activity was evaluated using DPPH free radical scavenging assay. The concentrations of the samples and α-tocopherol, a positive control were adjusted to the concentrations of 500, 250, 125, 62.

성능/효과

However, the nonpolar n-hexane and CH₂Cl₂ fractions indicated very low inhibition compared with acarbose, the positive control. The EtOAc fraction showed very good capability as almost 100% compared with the control at the higher concentrations than 12.5 ppm and the crude fraction also indicated good potential as 95% and 100%, at 25 and 50 ppm, respectively, compared with the control at 25 and 50 ppm. The H₂O fraction too gave good inhibition value as 90% at 50 ppm although the value was lower than the control.
These results indicate that the polar fractions containing a large amount of polyphenolic compounds have better α-glucosidase inhibition activities and that the antioxidant and antidiabetic activities have a positive correlation.

후속연구

According to the above results, the extracts of katsura tree leaves can be used as a potential natural resource substituting with synthetic antioxidant or antidiabetic medicine. Also there should be further studies to investigate the index compounds concerning in the biological activities and to make functional or medicinal output using the species.

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