선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- 5% HCl). For the intra-day variability, five replicas of each concentration level were analyzed in a day, and the intra-day variability was evaluated by analyzing each concentration level of standard solutions in duplicate for five consecutive days. The amounts of C3G and C3R were calculated from the corresponding calibration curves, and the relative standard deviation (RSD%) was taken as a measure of precision.
- General experimental procedures − HPLC analyses were carried out on a Waters Alliance HPLC (Waters, Milford, MA, USA) composed of a binary pump, an online degasser, and an autosampler.
- Linearity, limit of detection (LOD), and limit of quantification (LOQ) − The stock standard solutions of C3G and C3R (1 mg/mL each) were diluted with methanol (0.5% HCl) to cover the concentration range 1.25 - 500µg/mL and injected in the HPLC to evaluate the LOD and LOQ.
- For the intra-day variability, five replicas of each concentration level were analyzed in a day, and the intra-day variability was evaluated by analyzing each concentration level of standard solutions in duplicate for five consecutive days. The amounts of C3G and C3R were calculated from the corresponding calibration curves, and the relative standard deviation (RSD%) was taken as a measure of precision.
이론/모형
- Three concentration levels of working standard solutions of C3G and C3R mixtures [50 (low), 200 (medium), and 400 µg/mL (high)] were added to the known amounts of mulberry sample solution. The spiked samples were filtered and analyzed five times by using the described HPLC method. The amounts of C3G and C3R of each spiked solution were calculated from the corresponding calibration curves.
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