[논문]Fungal Endophytes Isolated from Needle Leaves of Three Coniferous Species on Mt. Seodae of Korea

Eo, Ju-Kyeong; Kim, Chang-Kyun; Eom, Ahn-Heum

doi:10.4489/kjm.2015.43.2.133

Fungal Endophytes Isolated from Needle Leaves of Three Coniferous Species on Mt. Seodae of Korea 원문보기

한국균학회지 = The Korean journal of mycology, v.43 no.2, 2015년, pp.133 - 136

Eo, Ju-Kyeong (Department of Biology Education, Korea National University of Education) , Kim, Chang-Kyun (Department of Biology Education, Korea National University of Education) , Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)

Abstract ▼ AI-Helper

We investigated endophytic fungi diversity of two Pinaceae species (Pinus densiflora, Pinus rigida) and one Cupressaceae species (Juniperus rigida) on Mt. Seodae, Korea. In total, 34 isolates were obtained from 19 host plants and identified according to internal transcribed spacer (ITS) region. As a result, they were placed in 13 taxa. Among them, 52.9% belonged to Leotiomycetes, 32.3% belonged to Sordariomycetes, 5.8% belonged to Agaricomycetes, and 3.0% for isolates belonged to either Eurotiomycetes, Dothideomycetes or Ustilaginomycetes. In particular, Lophodermium growing in pine would be an especially important instance of biodiversity for endophytes, suggesting that further study examining its ecological function in the environment is critical.

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문제 정의

Currently, global climate change is affecting forest decline[7], thus studies into the relationships between conifer species and their symbionts, sharing an evolutionary history, are needed to biodiversity. The purpose of this study was to isolate and identify endophytic fungi from needle leaves to compare their diversities among different coniferous species in Korea.

제안 방법

PCR products were examined by electrophoresis with 1.5% agarose gel using RedSafe gel stain (iNtRON Biotechnology, Seongnam, Korea), the amplified bands’ images were captured by Digital Gel Documentation (Korea Bio-Tech, Korea), and sequencing was conducted in SolGent (Daejeon, Korea).
To identify them, we selected the taxon with the greatest identity. We then aligned these sequences to construct a phylogenetic tree by neighbor-joining method to confirm their topological position on the tree (Fig. 1). Bootstrap analysis was performed with 1,000 replicates using MEGA5 [9] and outgroup was set as Glomus intraradices to complete the tree.

대상 데이터

Sampling and Isolation Leaf samples were collected in Mt. Seodae (36° 13 N, 127° 56 E), which is located in Chungnam Province.

성능/효과

The largest portion fungal isolates identified were found to be P. rigida, but species diversity in J. rigida (Shannon diversity index; H´ = 1.79) is higher than that in P. rigida (H´ = 0.78) (Table 2).
Meanwhile, specificity of endophytes for woody plants has been seldom reported because many fungal endophytes experience horizontal transfer and this yields a highly random distribution of endophytes. This study has set a precedent for the existence of a host-specific taxon, Lophodermium, and our results also suggest that certain taxa are affected by environmental factors other than host specificity. Many endophytes will be distributed to new biological niches via the various interactions among them.

참고문헌 (14)

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