Decursin and decursinol angelate가 2세대 출산률 회복에 미치는 영향 Recovery from the Two-generation Reproductive Toxicity in Sprague-Dawley Rats by Treatment with Decursin and Decursinol Angelate원문보기
2세대동물에 있어 bisphenol A (BPA)에 대하여 decursin and decursinol angelate (D/DA)의 투여가 출산률 회복에 미치는 영향에 대하여 연구하였다. 실험그룹은 대조군, BPA 투여군, BPA와 D/DA 병용투여군으로 이루어졌으며, BPA와 D/DA 병용투여군에서 BPA 투여군과 비교 시 어미개체에서 몸무게, 장기무게에서 차이를 보였다. 정자수는 대조군과 비교 시 BPA군에서는 64.70%로 감소하였고 BPA와 D/DA 병용투여군에서는 7.69% 감소를 확인하였다. 고용량의 BPA (50 mg/kg/day)의 복용개체에서는 1세대에 임신 및 출산을 하지 못하였다. 하지만, BPA와 D/DA 병용투여군에서는 BPA 투여군과 비교 시 임신(98.87%) 및 출산률(55.78%)이 회복 됨을 확인하였다. 또한, 2세대의 임신 및 출산률은 대조군(75.02%)과 비교 시 BPA와 D/DA 병용투여군(78.11%)에서는 차이가 없음을 확인하였다. 이 연구결과는 D/DA (50 mg/kg/day)의복용은 임신 및 출산에 있어 긍정적인 영향을 주는 것으로 확인하였다.
2세대동물에 있어 bisphenol A (BPA)에 대하여 decursin and decursinol angelate (D/DA)의 투여가 출산률 회복에 미치는 영향에 대하여 연구하였다. 실험그룹은 대조군, BPA 투여군, BPA와 D/DA 병용투여군으로 이루어졌으며, BPA와 D/DA 병용투여군에서 BPA 투여군과 비교 시 어미개체에서 몸무게, 장기무게에서 차이를 보였다. 정자수는 대조군과 비교 시 BPA군에서는 64.70%로 감소하였고 BPA와 D/DA 병용투여군에서는 7.69% 감소를 확인하였다. 고용량의 BPA (50 mg/kg/day)의 복용개체에서는 1세대에 임신 및 출산을 하지 못하였다. 하지만, BPA와 D/DA 병용투여군에서는 BPA 투여군과 비교 시 임신(98.87%) 및 출산률(55.78%)이 회복 됨을 확인하였다. 또한, 2세대의 임신 및 출산률은 대조군(75.02%)과 비교 시 BPA와 D/DA 병용투여군(78.11%)에서는 차이가 없음을 확인하였다. 이 연구결과는 D/DA (50 mg/kg/day)의복용은 임신 및 출산에 있어 긍정적인 영향을 주는 것으로 확인하였다.
The aim of this study was to determine the effect of decursin (D) and decursinol angelate (DA) against bisphenol A (BPA) toxicity in a rat two-generation study. Adult rats were divided into the following three groups: (1) control, (2) BPA, and (3) BPA+D/DA. The D and DA treatment of F0 parents incre...
The aim of this study was to determine the effect of decursin (D) and decursinol angelate (DA) against bisphenol A (BPA) toxicity in a rat two-generation study. Adult rats were divided into the following three groups: (1) control, (2) BPA, and (3) BPA+D/DA. The D and DA treatment of F0 parents increased the terminal body weight and relative adult organ weights (testes, kidneys, spleen, and liver) when compared with the BPA group. A significant decrease in sperm count was found in the BPA+D/DA (7.69%) and BPA (64.70%, p<0.01) groups, when compared with the sperm count in the control group. No offspring were obtained in the F1 generation of the BPA (50 mg/kg/day) group, but the addition of D/DA in the BPA+D/DA group significantly restored fertility (55.78%) and gestation indices (98.87%) in the F1 generation. No significant differences were found in the fertility index between the control (75.02%) and the BPA+D/DA (78.11%) groups in the two-generation study, when compared with the one-generation study. The viability ratio during lactation in the D/DA group was also similar to that of the control group. These data indicate that D/DA (50 mg/kg/day) administered over two generations causes significant positive changes in reproductive or developmental parameters.
The aim of this study was to determine the effect of decursin (D) and decursinol angelate (DA) against bisphenol A (BPA) toxicity in a rat two-generation study. Adult rats were divided into the following three groups: (1) control, (2) BPA, and (3) BPA+D/DA. The D and DA treatment of F0 parents increased the terminal body weight and relative adult organ weights (testes, kidneys, spleen, and liver) when compared with the BPA group. A significant decrease in sperm count was found in the BPA+D/DA (7.69%) and BPA (64.70%, p<0.01) groups, when compared with the sperm count in the control group. No offspring were obtained in the F1 generation of the BPA (50 mg/kg/day) group, but the addition of D/DA in the BPA+D/DA group significantly restored fertility (55.78%) and gestation indices (98.87%) in the F1 generation. No significant differences were found in the fertility index between the control (75.02%) and the BPA+D/DA (78.11%) groups in the two-generation study, when compared with the one-generation study. The viability ratio during lactation in the D/DA group was also similar to that of the control group. These data indicate that D/DA (50 mg/kg/day) administered over two generations causes significant positive changes in reproductive or developmental parameters.
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문제 정의
sperm cells [11]. In this study, we studied the effects of exposure of rat to D/DA on reproduction and develop- ment in a two-generation reproductive toxicity study.
제안 방법
Among one-generation, 10 male rats were used for testing in two-generation. All generation babies were evaluated by the number of pups of litter, fer- tility index, gestation index, and viability index after main- taining with normal condition. During mating period, vagi- nal smears were identified for the presence of sperm.
대상 데이터
Male and female Sprague-Dawley (SD) rats at 4 weeks of age were used in this experiment. Experimental protocol (number: 2010-003A) was approved by the Institutional Animal Care and Use Committee of the Kyungsung University.
데이터처리
). Statistical analyses were performed using the Sigma Plot 10.0 program. Values were compared to control by per- forming analysis of variance (ANOVA) followed by un- paired Student's t-test.
0 program. Values were compared to control by per- forming analysis of variance (ANOVA) followed by un- paired Student's t-test. p values <0.
성능/효과
However, there was con- sistently affected in F1 offspring in BPA group. In the BPA+ D/DA group, the terminal body weights of F0 (17.98%, p<0.05) and F1 (57.76%, p<0.01) decreased and the weights of the testes F0 (4.47%, p<0.05) and F1 (8.76%, p<0.05), the liver F0 (5.00%, p<0.05) and F1 (5.48%, p<0.05), the kidney F0 (10.61%, p<0.01) and F1 (8.13%, p<0.01), the spleen F0 (7.05%, p<0.01) and F1 (12.20%, p<0.01) increased sig- nificantly compared to the corresponding relative weights in the control group. Absolute and relative organ weight data for F1 weanling pups indicate that the absolute organ weights were reduced, and the relative weights were in- creased in BPA+ D/DA group.
These re- sults indicate that oral doses of 50 ㎎/㎏/day of D/DA recover damaged reproductive parameters by BPA over two-generation. More importantly, although organ weights of F0 and F1 males in the BPA+D/DA and BPA groups were different from those in the control group, the evidence of organ weights by D/DA (F2 males) affected, in as much as the overall ANOVA showed significant differences (p< 0.05 and p<0.01) between the BPA+D/ DA and BPA groups.
5 to 2 times in the food consumption com- pared to other doses. These data indicated that the weights of the kidney, liver, spleen and testes were significantly cor- related (p<0.05 and p<0.01) with body weight. The organ weight of F2 males are presented in Table 1.
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