[논문]THP-1 세포에서 융합 발효 독활의 항염증 효과

정영미; 이동섭; 권기상

doi:10.14400/jdc.2016.14.1.353

THP-1 세포에서 융합 발효 독활의 항염증 효과
Anti-Inflammatory Effects of Fusion-Fermented Aralia continentalis Radix (fACR) on THP-1 cells 원문보기

디지털융복합연구 = Journal of digital convergence, v.14 no.1, 2016년, pp.353 - 361

정영미 (경북대학교 유전공학과) , 이동섭 (경운대학교 보건바이오학과) , 권기상 (경운대학교 임상병리학과)

초록
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본 연구는 독활의 기능을 부가시키고자 발효과정을 통해 융합-발효 추물물을 얻어 항염증효과를 확인 하고자 한다. 독활을 이용하는데 한계가 있어 유산균주들을 활용한 발효과정을 통해 이용 가능한 균주를 선별하고, 독활의 열수 추출물과 비교하여 총 폴리페놀, 아미노산 총량, 주요 생리활성 미네랄 성분 분석 결과 발효과정을 통해 증가함을 확인하였다. 독활의 발효추출물의 항염증 효과를 확인하고자 관절염 염증반응과 관련된 사이토카인들을 측정하였고, 관절염시 주로 동반되는 연골조직 파괴를 억제 효능을 평가하기 위해 MMP-9 활성도를 확인하였다. 이러한 결과들은 유산균을 이용한 발효과정을 통해 독활의 주요 생리활성이 증가하고 염증반응 억제 효과도 증가하는 것으로 보여진다. 이 연구는 독활의 관절염 예방적 효과를 연구하는데 있어 기초 연구가 될 것으로 기대된다.

Abstract ▼ AI-Helper

This study was to demonstrate the anti-inflammation effect using extracts derived from fusion-fermentation to add the function of Araliae Continentalis Radix (fACR). Since there are limitations to the use of ACR, available strains were selected through fermentation using lactobacillus strains, and the increases in total amount of polyphenols and amino acids was confirmed through comparison with Hot Water Extract of ACR. To determine the anti-inflammatory effect of the fACR was measure cytokine inflammation associated with arthritis, the arthritis when cartilage destruction is accompanied mainly MMP-9 activity was confirmed to evaluate the inhibition effect. These results show that fermentation using lactobacillus increases major biological activities and inflammatory response-restraining effects of ACR. This study is expected to be a basis for studying the preventive effect of fACR on arthritis.

주제어

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제안 방법

Test report about total polyphenol and amino acid contents was entrusted to and prepared by Suwon Women's University Food Analysis Research Center. An analysis of major biologically-active mineral components and heavy metals contents was entrusted to and conducted by Entech Analysis Research Institute Co., Ltd. and subsequently inspection report was prepared.
To overcome the limitations of useful Araliae Continentalis Radix to the use, this study used fermentation. Continentalic acid, an index component, somewhat increased during the fermentation of multiple lactobacillus of LGG and LP.
Using THP-1 cell line which is a human monocyte cell, the effects of fACR on the expressions of hTNF-alpha and hIL-8 among cytokines related to inflammatory responses in arthritis and hMCP-1, as chemokine, known to be related to the induction of arthritis pain were analyzed to confirmation inflammatory response-restraining effects of fACR. fACR and ACR were each dissolved at a concentration of 10, 30, 50, 100, 300 and 500 μg/ml in triple distilled water, and the sample was treated in THP-1 cells.
Inflammation including arthritis is a biological response to various types of infections or stimulating materials among metabolites in a human body, and different mediators such as NO, free radicals, cytokines, PGE2 and lysosomal enzyme are involved in it. Using THP-1 cells, the effects of fACR on the expressions of hTNF-alpha and hIL-8 among cytokines related to inflammatory responses in arthritis and hMCP-1, as chemokine, known to be related to the induction of arthritis pain were analyzed to confirmation inflammatory response-restraining effects of fACR. The hTNF-alpha represents cell toxicity in tumor cell and has relation to chronic inflammation, and it is an inductive factor of cytokines including IL-8.

대상 데이터

Dried Araliae Continentalis Radix, which was purchased in Pung-gi Market, Yeongju city, Gyeongbuk province, was washed and used. After hot water extraction at for 10 minutes of 4% Araliae Continentalis Radix, the Araliae Continentalis Radix was inoculated with each strain, and this strain was cultured.
Test report about total polyphenol and amino acid contents was entrusted to and prepared by Suwon Women's University Food Analysis Research Center.

성능/효과

An analysis on total polyphenol content was entrusted to and conducted by Food Analysis and Research of Center of Suwon Women’s University, which is an authorized analysis organization. As a result, it is found that total polyphenol content related to antioxidation contained in fACR increased remarkably to 48.63 mg/100g compared to ACR 29.34 mg/100g, which was not fermented, under the same conditions. Also, through a component analysis on major amino acid content, it is found that total amino acid content in fACR increased to 132.
The hTNF-alpha represents cell toxicity in tumor cell and has relation to chronic inflammation, and it is an inductive factor of cytokines including IL-8. As the results of a measurement of hTNF-alpha secretion rate using ELISA after inducing inflammation with LPS, it is found that the secretion rates of hTNF-alpha and hIL-8 were significantly inhibited in fACR. The hIL-8 is found in inflammatory diseases including rheumatism, and affects inflammation-related cells such as neutrophe, B lymphocyte and eosinophil.
There is little research on biological and pharmacological activity increasing through fermentedAraliae Continentalis Radix (fACR). Thus, this study found out fermentation microorganism suitable for enhancing anti-inflammation and antioxidant effect of Araliae Continentalis Radix, as natural substance, used for oriental medicine, and also confirmed the increases in biological activities and anti-inflammation effect based on fermentation by comparing Araliae Continentalis Radix -Hot Water Extract (ACR) and fACR.

참고문헌 (20)

N. H. Dang, X. F. Zhang, M. S. Zheng, K. H. Son, H. W. Chang, H. P. Kim, K. H. Bae, S. S. Kang, " Inhibitory constituents against cyclooxygenases from Aralia cordata Thunb", Arch Pharm Res, Vol. 28, pp. 28-33, 2005.

원문보기 상세보기
W. S. Han, "Isolation of the antimicrobial compounds from Aralia cordata Thunb. extract", Koren J, Medicinal Crop Sci Vol. 13, pp. 182-185, 2005.
S. I. Jeong, W. S. Han, Y. H. Yun, K. J. Kim, "Continentalic acid from Aralia continentalis shows activity against methicillin-resistant Staphylococcus aureus", Phytother Res, Vol. 20, pp. 511-514, 2006.

상세보기
H. J. Park, M. S. Hong, J. S. Lee, K. H. Leem, C. J. Kim,J. W. Kim and S. Lim, "Effects of Aralia continentalis on hyperalgesia with peripheral inflammation", Phytother Res Vol. 19, pp. 511-513, 2005.

상세보기
B. S. Jeon, J. W. Park, B. K. Kim, H. K. Kim, T. S. Jung, J. R. Hahm, D. R. Kim, Y. S. Cho, J. Y. Cha, "Fermented mushroom milk supplemented dietary fiber prevents the onset of obesity and hypertriglyceridemia in Otsuka Long-Evans Tokushima Fatty (OLETF) rats", Diabetes Obes Metab, Vol. 7, pp. 709-715, 2005.

상세보기
H. T. Trinh, S. J. Han, S. W. Kim, Y. C. Lee, D. H. Kim, "Bifidus fermentation increases hypolipidemic and hypoglycemic effects of red ginseng", J Microbiol Biotechnol, Vol. 17, No. 7, pp. 1127-1133, 2007.

원문보기 상세보기
S. I. Lee, J. G. Shin, S. D. Kim, "Effect of ginsengchungkukjang effects on lipid profiles of serum in alcohol administered diabetic-induced rats", J. Korean Soc Food Sci Nutr, Vol. 34, pp. 1362-1366, 2005.

원문보기 상세보기
S. Y. Kim, S. I. Jeong, S. Z. Kim, J. S. Shim, S. I. Jang, "Effects of Continentalic from Aralia Continentalis on Growth Inhibition and Apoptosis Induction in Human Leukemia HL-60 Cells", Korean J Oriental Physiol & Pathol, Vol. 23, No. 6, pp. 1314-1319, 2009.
L. M. Baek, L. Y. Park, K. S. Park, S. H. Lee, "Effect of Starter Cultures on the Fermentative Characteristics of Cheonggukjang", Korean J Food Sci Technol, Vol. 40, No. 4, pp. 400-405, 2008.
E. H. Han, T. S. Lee, B. S. Noh, D. S. Lee, "Volatile flavor components in mash of takju prepared by using different nuruks", Korean J Food Sci Technol, Vol. 29, pp. 563-570, 1997.
H. R. Park, S. J. Hong, "Research on Natural Medicine for Wellness and Oral Health", J Digital Convergence Vol.13, No.5, pp.357-363, 2015.
Y. H. Pyo, "Effect of Monascus-fermentation on the content of GABA and free amino acids in soybean", J Korean Soc Food Sci Nutr, Vol. 37, pp. 1208-1213, 2008.

원문보기 상세보기
J. H. Lee, J. S. Park, "Antioxidant Activities of Rice Bran Extracts for Wellness Convergence", J Digital Convergence, Vol.13, No. 2 pp.401-406, 2015.
M. L. Buckley, D. P. Ramji, "The influence of dysfunctional signaling and lipid homeostasis in mediating the inflammatory responses during atherosclerosis", Biochim Biophys Acta, Vol. 1852, No. 7, pp.1498-1510, 2015.

상세보기
Michael Schuliga, "NF-kappaB Signaling in Chronic Inflammatory Airway Disease", Biomolecules, Vol. 5, No. 3, pp. 1266-1283, 2015.

상세보기
C. Y. Wenham, M. McDermott, P. G. Conaghan, "Biological Therapies in Osteoarthritis", Curr Pharm Des, Vol. 21, No. 17, pp. 2206-2215, 2015.

상세보기
G. P. Amorino, R. L. Hoover, "Interactions of monocytic cells with human endothelial cells stimulate monocytic metalloproteinase production", Am J Pathol, Vol. 152, pp. 199-207, 1998.

상세보기
C. N. Andrew, "Metalloproteinases expression in monocytes and macrophages and its relationship to atherosclerotic plaque instability", Arterioscler Thromb Vasc Biol, Vol. 28, pp. 2108 -2114, 2008.

상세보기
A. Bar-Or, R. K. Nuttall, M. Duddy, A. Alter, H. J. Kim, I. Ifergan, C. J. Pennington, P. Bourgoin, D. R. Edwards, V. W. Yong, "Analyses of all matrix metalloproteinase members in leukocytes emphasize monocytes as major inflammatory mediators in multiple sclerosis", Brain, Vol. 126, pp. 2738-2749, 2003.

상세보기
J. Lim, Y. Cho, D. H. Lee, B. H. Jung, H. S. Kang, T. J. Kim, K. J. Rhee, T. U. Kim, Y. S. Kim, "Upregulation of MMP is Mediated by MEK1 Activation During Differentiation of Monocyte into Macrophage", Exp Biomed Sci, Vol. 18, No. 2, pp. 104-111, 2012.

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