[논문]LED조사가 인간 피부 각질세포의 증식에 미치는 융복합적인 영향

박정숙; 김미혜; 이재혁

doi:10.14400/jdc.2016.14.11.639

LED조사가 인간 피부 각질세포의 증식에 미치는 융복합적인 영향
Effect of LED Irradiation on Proliferation of Human Epidermal Keratinocyte for Convergence 원문보기

디지털융복합연구 = Journal of digital convergence, v.14 no.11, 2016년, pp.639 - 644

박정숙 (남부대학교 간호학과) , 김미혜 (중부대학교 미용분장학과) , 이재혁 (남부대학교 한방제약개발학과)

초록
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본 연구의 목적은 발광 다이오드 (LED)을 인간 피부 각질 세포에 조사 시 콜라겐, 프로 콜라겐의 증식 발현을 조사하기 위해 실시되었었다. LED 조사 시 안전하게 인간의 피부에 적용할 수 있는지 여부를 결정하기 위해, LED 조사의 증식 효과는 인간 표피 각질세포에서 MTS 분석으로 결정하였다. 470nm의 파장 조사는 세포 독성 없이 mRNA의 콜라겐의 발현, 프로 콜라겐을 증가시켰으며, 이 결과는 470nm LED 조사가 피부각질세포 증식 효과와 콜라겐 합성에 영향을 미칠 수 있음을 시사한다. 또한 LED 조사시 독성 효과는 인간 피부 섬유 아세포 (HDF)에서 MTS 분석으로 결정 한 결과 세포 증식에 독성을 나타내지 않았다. 470nm LED 조사시 시험 관내 콜라겐 합성 활동을 증가시킴으로 피부미용 및 융복합적인 부분에 활용할 수 있는 기초 자료로 활용이 가능하다고 사료된다.

Abstract ▼ AI-Helper

The purpose of this study is to determine the effect of the light-emitting-diode (LED) to investigate proliferation of human epidermal keratinocyte and collagen, procollagen expression. In order to determine whether LED irradiation can safely be applied to human skin, the proliferative effects of LED irradiation were determined by MTS assay in Human Epidermal Keratinocytes. Wavelength of 470nm LED irradiation increased mRNA expression of collagen, procollagen without cytotoxity. Our results suggest that 470nm LED irradiation may have a proliferative effects and collagen synthesis property. In order to determine whether LED irradiation can safely be applied to human skin, the cytotoxic effects of LED irradiation were determined by MTS assay in Human Dermal Fibroblasts (HDF). As far as we know, this is the first report demonstrating in vitro collagen synthesis activity of 470nm LED irradiation and being a scientific basis for the cosmetic.

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문제 정의

As far as we know, this is the first report demonstrating in vitro collagen synthesis activity of 470nm LED irradiation and being a scientific basis for its cosmetic.
Cell viability effects of 470nm, 525nm, 630nm was measured using a solution for using the CellTiter 96® colorimetric by counting the number of viable cells proliferation. This analysis was performed to determine the number of viable cells remaining after the completion of the culture process. Human epidermal keratinocytes play in a 96-well flat bottom plates at a density of 1 × 10⁴cells of the well, 470nm to 525nm and 630nm light irradiation after a first cell culture 24hours after the time of the manufacturer of the number of viable cells.

제안 방법

So we selected the wavelength in the visible rays which the side effect is not reported until now in this research and in order to replace the risk of the laser injuring the skin tissue and eye[19,20]. In this research, by using the LED light source of the visible ray wavelengths 470nm, 525nm and 630nm, we proved the collagen synthesis effect of LED at human epidermal keratinocyte(neonatal, HEKn). As far as we know, this is the first report demonstrating in vitro collagen synthesis activity of 470nm LED irradiation and being a scientific basis for its cosmetic.

대상 데이터

Human epidermal keratinocytes (neonatal) (HEKn), animal product free EpiLife® basal medium and growth supplements (HKGS), recombinant trypsin/EDTA, defrinded trypsin inhibitor, and streptomycin had bought from Gibco (NY, USA). Human skin fibroblasts (HDF) was taken at Chungnam National University, Korea. HDF is then incubated with Dulbecco's modified Eagle's medium, penicillin 100 U / ㎖, supplement (DMEM GIBCO Inc.

이론/모형

Human epidermal keratinocytes were disposed of 470nm(Blue), 525nm(Green), 630nm(Red) LED irradiation for 1 hr. Cell viability was measured by MTS assay. The results of experiment three times are indicated by three independent experiments (SD = bars) percent of the average cell viability compared to the average, and the untreated control cell and survival of the asterisk denotes a significant difference
After the secondary antibody conjugated for 1 hour at room temperature after the incubation by joining and horseradish PBS with peroxidase. It was blocked by PBS-T. The protein-enhanced chemiluminescence (Pierce) and image quant method was visualized by LAS 4000 (GE Healthcare, USA).

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LED조사가 인간 피부 각질세포의 증식에 미치는 융복합적인 영향
Effect of LED Irradiation on Proliferation of Human Epidermal Keratinocyte for Convergence 원문보기

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LED조사가 인간 피부 각질세포의 증식에 미치는 융복합적인 영향 Effect of LED Irradiation on Proliferation of Human Epidermal Keratinocyte for Convergence 원문보기

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LED조사가 인간 피부 각질세포의 증식에 미치는 융복합적인 영향
Effect of LED Irradiation on Proliferation of Human Epidermal Keratinocyte for Convergence 원문보기

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