[논문]Potential prevention effects of Rubus occidentalis seed on UVB-induced MMP-1 production and procollagen degradation in CCD-986sk cells

Kim, Dong-Hee; Park, Tae-Soon; Son, Jun-Ho

doi:10.3839/jabc.2016.054

Potential prevention effects of Rubus occidentalis seed on UVB-induced MMP-1 production and procollagen degradation in CCD-986sk cells 원문보기

Journal of applied biological chemistry, v.59 no.4, 2016년, pp.317 - 322

Kim, Dong-Hee (Traditional Korean Medicine Technology Division R&D Department Applied Product Development Team, National Development Institute of Korean Medicine) , Park, Tae-Soon (Traditional Korean Medicine Technology Division R&D Department Applied Product Development Team, National Development Institute of Korean Medicine) , Son, Jun-Ho (Traditional Korean Medicine Technology Division R&D Department Applied Product Development Team, National Development Institute of Korean Medicine)

Abstract ▼ AI-Helper

UV exposure induces matrix metalloproteinases (MMPs) and extracellular matrix-degrading enzymes expression. We studied the protective effect of Rubus occidentalis seed against UVB-generated skin photoaging using human fibroblast cells (CCD-986sk). We used an ELISA kit to measure the supernatents of procollagen type I and MMP-1 in CCD-986sk cells after they were exposed to UVB irradiation. The CCD-986sk cells that were used with RC-E/E after the UVB irradiation caused higher levels of type I procollagen and lesser levels of MMP-1 compared with the control group. Furthermore, the RC-E/E treated group showed lesser MMP-1 levels and higher procollagen type I levels than the untreated counterpart. Therefore, it can be concluded that Rubus occidentalis seed can prevent from skin photoaging.

주제어

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제안 방법

Two μg of RNA were used for the 1 h cDNA synthesis that involved the use of oligo (dT) primer and reverse transcriptase (Promega, WI, USA) at 42 ℃. A PCR was performed with the cDNA to analyze the transcript expressions of MMP-1, MMP-2, and MMP-3, where by a 55 ℃ annealing temperature was applied for 30 sec and 72 ℃ was applied for a 1 min extension; the total for the PCR is 30 cycles. The transcripts were separated in a 1.
A PCR was performed with the cDNA to analyze the transcript expressions of MMP-1, MMP-2, and MMP-3, where by a 55 ℃ annealing temperature was applied for 30 sec and 72 ℃ was applied for a 1 min extension; the total for the PCR is 30 cycles. The transcripts were separated in a 1.5 % agarose gel and stained with Redsafe (iNtRON Biotechnology Inc., Sungnam, Gyeonggi, Korea), followed by the use of a LAS4000 (Fujifilm, Tokyo, Japan) image analyzer for the confirmation.

대상 데이터

The R. occidentalis seeds were obtained from the “Sam do bong Farm” company that is located in Kimcheon, Gyeongbuk, Korea.
This study was conducted in triplicate. An analysis of variance was run using the raw data, and the mean values and standard deviations were analyzed using the ANOVA system.

데이터처리

This study was conducted in triplicate. An analysis of variance was run using the raw data, and the mean values and standard deviations were analyzed using the ANOVA system. Differences among the means were determined by a t-test, and significance occurs at *p <0.

이론/모형

occidentalisseed. Cell viability was measured using an MTT assay. Error bar indicates average mean of triple replicates.

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