[논문]저장기간 중 돌산갓피클에서 Glucosinolates의 ACE 및 α-glucosidase 저해활성

손혜련; 오선경; 최명락

doi:10.7841/ksbbj.2016.31.3.165

[국내논문] 저장기간 중 돌산갓피클에서 Glucosinolates의 ACE 및 α-glucosidase 저해활성
ACE and α-glucosidase Inhibitory Activity of the Glucosinolates in Dolsan Leaf Mustard Pickle during Storage 원문보기

KSBB Journal, v.31 no.3, 2016년, pp.165 - 170

손혜련 (전남대학교 바이오전자메디컬협동과정) , 오선경 (전남대학교 바이오전자메디컬협동과정) , (일본 이와테대학교 농학부 응용생물화학과정) , 최명락 (전남대학교 바이오전자메디컬협동과정)

Abstract ▼ AI-Helper

This study was carried out for investigate that physiological activity, quantification and qualitative were sinigrin of Dolsan leaf mustard pickle (DLMP) during storage. sinigrin contains high amounts of nutritional and medicinal compounds, which are important for maintaining optimum health. ACE inhibitory activity was ranged between 43.2 and 79.4%. DLMP methanol extracts demonstrated highest ACE inhibitory activity at 79.4% on day 14, whereas DLMP ethanol extracts demonstrated highest Angiotensin I-converting Enzyme (ACE) inhibitory activity of 43.2% at day 0. The ${\alpha}-glucosidase$ inhibitory activity of positive control 0.02% (v/v) acarbose was 78%. The DLMP methanol extracts had the highest ${\alpha}-glucosidase$ inhibitory activity at 64.0% on day 14, whereas DLMP ethanol extracts had the lowest ${\alpha}-glucosidase$ inhibitory activity of 42.8% at day 28. Sinigrin was high in DLMP methanol extracts at $49.55{\mu}g/ml$ on day 14 of storage. Sinigrin standard was eluted at 2.73 min and MS analysis was m/z 283.03 along with fragment ions at m/z 204 and 149.06. These data show that sinigrin formed desulfo-glucosinolates $[M-SO_3-2H_2O+K+2H]^+$. Sinigrin concentration increased until day 14 and then decreased after that. DLMP methanol extracts had consistently higher sinigrin concentration than DLMP acetonitrile extracts during 28 days of storage.

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가설 설정

2)All values are mean±SD of the triplicate determination.

제안 방법

In this study, Dolsan leaf mustard pickle (DLMP) extracts were for α-glucosidase and ACE inhibitory activities.
LC-PDA/MS/MS analysis was performed by Shimadzu Prominence UPLC with a Thermo Orbitrap XL system (Thermo Fisher Scientific, Bremen, Germany). The LC column used was Phenomenex KINETEX C18 (150 mm × 2.
DLMP samples were prepared with Dolsan leaf mustard (65%), soy (10%), apple vinegar (7%), water (12%), garlic (1%) and condiment sauce (5%), and stored at 4℃ for 28 days after preparation.
In this study, Dolsan leaf mustard pickle (DLMP) extracts were for α-glucosidase and ACE inhibitory activities. In addition, qualitative and quantitative was conducted for the DLMP extracted sinigrin of glucosinolates by using liquid chromatography with photodiode array and tandem mass spectrometry (LC-PDA/MS/MS).

대상 데이터

Dolsan leaf mustard was produced at Yeosu-si Dolsan-eup, Korea and harvested in October 2015. The harvested Dolsan leaf mustard was blanched at 80℃ for 10 s.
This study was supported by the BK21 program.
Rabbit lung acetone powder (ACE-8.2 units/g), Hippuryl-LHistidiyl-L-Leucine (Hip-His-Leu), α-glucosidase, p-Nitrophenyl-α-D-glucopyranoside (p-NPG) and acarbose were purchased from Sigma-Aldrich (St. Louis, MO, USA).

데이터처리

One way analysis of variance (ANOVA) and Duncan’s test were used for multiple comparisons with SPSS version 21.0 (SPSS Institute, Chicago, IL, USA).
Means in row (a-c) and a column (A-E) followed by different superscripts are significantly different at p<0.05 by Duncan’s multiple range test.
Means in row (a-b) and a column (A-E) followed by different superscripts are significantly different at p<0.05 by Duncan’s multiple range test.

참고문헌 (20)

Cho, Y. S., B. S. Ha, S. K. Park, and S. S. Chun (1993) Contents of carotenoids and chlorophylls in Dolsan leaf mustard (Brassica juncea). Korean J. Diet. Cult. 8: 153-157.
Li, G. H., G. W. Le, Y. H. Shi, and S. Shrestha (2004) Angiotensin I-converting enzyme inhibitory peptides derived from food proteins and their physiological and pharmacological effects. Nutr. Res. 24: 469-486.

상세보기
Vermeirssen, V., A. van der Bent, J. Van Camp, A. van Amerongen, and W. Verstraete (2004) A quantitative in silico analysis calculates the angiotensin I converting enzyme (ACE) inhibitory activity in pea and whey protein digests. Biochimie 86: 231-239.

상세보기
Alper, J. (2000) New insights into type 2 diabetes. Science 289: 37.

상세보기
Baron, A. D. (1998) Postprandial hyperglycaemia and alpha-glucosidase inhibitors. Diabetes Res. Clin. Pract. 40: 51.

상세보기
Mooradian, A. D. and J. E. Thurman (1999) Drug therapy of postprandial hyperglycemia. Drugs. 57: 19.
Watanabe, J., J. Kawabata, H. Kurihara, and R. Niki (1997) Isolation and identification of alpha-glucosidase inhibitors from Tochucha (Eucommia ulmoides). Biosci. Biotechnol. Biochem. 61: 177.

상세보기
Huang, W. Y., S. T. Davidge, and J. Wu (2013) Bioactive natural constituents from food sources-potential use in hypertension prevention and treatment. Crit. Rev. Food Sci. Nutr. 53: 615-630.

상세보기
Cushman, D. W. and H. S. Cheung (2001) Spectrophotometeric assay and properties of angiotensin converting enzyme of rabbit lung. Food Chem. 75: 237-240.

상세보기
Feng, J., X. W. Yang, and R. F. Wang (2011) Bio-assay guided isolation and identification of a-glucosidase inhibitors from the leaves of Aquilaria sinensis. Phytochemistry 72: 242-247.

상세보기
Choi, M. R., E. J. Yoo, S. H. Song, D. S. Kang, J. C. Park, and H. S. Lim (2001) Comparison of physiological activity in different parts of Dolsan leaf mustard. Korean J. Soc. Food Nutr. 30: 721-725.
Choi, M. R., E. J. Yoo, and H. S. Lim (2003) Effect of lactic acid bacterium on antioxidative and ACE inhibitory activity in Dolsan leaf mustard Kimchi. Korean J. Life Sci. 13: 59-66.

원문보기 상세보기
Liu, L., M. A. Deseo, C. Morris, K. M. Winter, and D. N. Leach (2011) Investigation of ${\alpha}$ -glucosidase inhibitory activity of wheat bran and germ. Food Chem. 126: 553-561.

상세보기
Ahn, B. G. (2007) Isolation of flavonoids and higher alcoholfrom the leaves of Brassica juncea andits ${\alpha}$ -glucosidase inhibitory activity. M.D. Thesis. University of Sunchon, Sunchon, Korea.
Kim, M. R. and H. S. Rhee (1989) Purification and characterization of radish myrosinase. Korea J. Food. Sci. Technol. 21: 136-144.
Son, H. R., S. K. Oh, S. O. Bae, and M. R. Choi (2015) Analysis of phycochemical and sensory properties of Dolsan leaf mustard pickles. Korean J. Life Sci. 25: 936-941.

원문보기 상세보기
Lim, H. S. (2002) The study for contents of sinigrin in Dolsan leaf mustard Kimchi during fermentation periods. Korean J. Life Sci. 12: 523-527.

원문보기 상세보기
Larsen, P. O. (1981) Glucosinolates in secondary plant products. Academic press, New york.
Tanaka, T., Y. Mori, Y. Morishita, A. Hara, T. Ohno, T. Kojima, and H. Mori (1990) Inhibitory effect of Sinigrin and indole-3-carbiol on diethylnitrosamine-induced hepatocarcinogenesis in mal ACI/N rats. Carcinogenesis 11: 1403-1406.

상세보기
Stephen, S., N. Hecht, J. Trushin, S. Rigotty, G. Carmella, A. Borukhova, S. Akerkar, and A. Rivenson (1996) Complete inhibition of 4-(methylnitrosamino)-1-(3-pyridyl)-1-buthan one-induced rat lung tumorigenesis and favorable modification of biomarkers by phenethyl isothiocyanate. Cancer Epidemiology Biomarker Prevention 5: 645-652.

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