식물정유의 당근뿌리혹선충(Meloidogyne hapla)에 대한 살선충활성 및 방향성성분 분석 Chemical Compositions and Nematicidal Activities of Essential Oils on Meloidogyne hapla (Nematoda: Tylenchida) Under Laboratory Conditions원문보기
본 연구는 뿌리혹선충의 친환경적 방제를 위하여 9종의 식물정유를 선발하여 당근뿌리혹선충 2령기 유충과 알에 대한 살선충 및 부화억제 효과를 검정하였다. 당근뿌리혹선충의 2령기 유충에 대해 $125{\mu}g/mL$로 검정한 결과, Alpinia galanga 정유가 100% 살선충활성을 나타냈고, Carum carbi (22.3%), Eugenia caryophyllata (9.4%), Cinnamonum zeylanicum (7.2%), Mentha pulegium (2.4%) 및 Foeniculum vulgare (2.1%) 순으로 활성을 나타내었다. 이들 중 A. galanga 정유를 이용하여 당근뿌리혹선충 알의 부화억제 활성을 검정한 결과, $500{\mu}g/mL$ 농도로 처리 후 7, 14, 21 일째까지 낮은 부화 활성을 나타내었다. 우수한 활성을 보인 A. galanga 정유의 활성 성분을 분석한 결과 methyl cinnamate 함유율이 가장 높았으며, 1,8-cineole, ${\beta}$-pinene, ${\alpha}$-pinene, p-cymene이 주요 방향성 물질로 구성되어 있음을 확인할 수 있었다. 따라서 본 연구에서 선발한 A. galanga 정유가 당근뿌리혹선충의 친환경적 방제수단으로 활용가능성이 있을 것으로 생각된다.
본 연구는 뿌리혹선충의 친환경적 방제를 위하여 9종의 식물정유를 선발하여 당근뿌리혹선충 2령기 유충과 알에 대한 살선충 및 부화억제 효과를 검정하였다. 당근뿌리혹선충의 2령기 유충에 대해 $125{\mu}g/mL$로 검정한 결과, Alpinia galanga 정유가 100% 살선충활성을 나타냈고, Carum carbi (22.3%), Eugenia caryophyllata (9.4%), Cinnamonum zeylanicum (7.2%), Mentha pulegium (2.4%) 및 Foeniculum vulgare (2.1%) 순으로 활성을 나타내었다. 이들 중 A. galanga 정유를 이용하여 당근뿌리혹선충 알의 부화억제 활성을 검정한 결과, $500{\mu}g/mL$ 농도로 처리 후 7, 14, 21 일째까지 낮은 부화 활성을 나타내었다. 우수한 활성을 보인 A. galanga 정유의 활성 성분을 분석한 결과 methyl cinnamate 함유율이 가장 높았으며, 1,8-cineole, ${\beta}$-pinene, ${\alpha}$-pinene, p-cymene이 주요 방향성 물질로 구성되어 있음을 확인할 수 있었다. 따라서 본 연구에서 선발한 A. galanga 정유가 당근뿌리혹선충의 친환경적 방제수단으로 활용가능성이 있을 것으로 생각된다.
To evaluate the efficacy of natural nematicides for the control of root-knot nematode in strawberry greenhouses, commercial essential oils were examined by 24-well culture plate bioassay for their nematicidal activities against second-stage juveniles and eggs of Meloidogyne hapla. Based on the morta...
To evaluate the efficacy of natural nematicides for the control of root-knot nematode in strawberry greenhouses, commercial essential oils were examined by 24-well culture plate bioassay for their nematicidal activities against second-stage juveniles and eggs of Meloidogyne hapla. Based on the mortality of M. hapla juveniles at a concentration of $125{\mu}g/mL$, the most active essential oil was Alpinia galanga (100%), followed by Carum carbi (22.3%), Eugenia caryophyllata (9.4%), Cinnamonum zeylanicum (7.2%), Mentha pulegium (2.4%), and Foeniculum vulgare (2.1%). Moreover, A. galanga significantly reduced hatching at 7, 14, and 21 days after treatment. The volatile constituents identified in the A. galangal oil were methyl cinnamate (87.4%), 1,8-cineole (4.4%), ${\beta}$-pinene (2.5%), ${\alpha}$-pinene (2.2%), and p-cymene (1.1%), as major constituents. Results of this study show that A. galangal essential oil and its major constituents may serve as an environmental friendly agent of a promising natural nematicide to control Meloidogyne spp.
To evaluate the efficacy of natural nematicides for the control of root-knot nematode in strawberry greenhouses, commercial essential oils were examined by 24-well culture plate bioassay for their nematicidal activities against second-stage juveniles and eggs of Meloidogyne hapla. Based on the mortality of M. hapla juveniles at a concentration of $125{\mu}g/mL$, the most active essential oil was Alpinia galanga (100%), followed by Carum carbi (22.3%), Eugenia caryophyllata (9.4%), Cinnamonum zeylanicum (7.2%), Mentha pulegium (2.4%), and Foeniculum vulgare (2.1%). Moreover, A. galanga significantly reduced hatching at 7, 14, and 21 days after treatment. The volatile constituents identified in the A. galangal oil were methyl cinnamate (87.4%), 1,8-cineole (4.4%), ${\beta}$-pinene (2.5%), ${\alpha}$-pinene (2.2%), and p-cymene (1.1%), as major constituents. Results of this study show that A. galangal essential oil and its major constituents may serve as an environmental friendly agent of a promising natural nematicide to control Meloidogyne spp.
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제안 방법
Because of potent activity of six essential oils (A. galanga, C. carbi, E. caryophyllata, C. zeylanicum, M. pulegium, and F. vulgare) against M. hapla juveniles, the hatching inhibition of selected oils to M. hapla eggs was investigated using 24-well culture plate bioassay at a concentration of 500 μg/mL (Fig. 1).
대상 데이터
Meoloidogyne hapla, originally extracted from a strawberry field in Gongju (Chungnam province, South Korea), was cultured on tomato (Lycopersicon esculentum cv. Rutgers) in a glasshouse. Egg masses were isolated from plant roots, collected in water, and sterilized by agitation for 3 min in 1% sodium hypochlorite (NaOCl) solution (Kim et al.
Nine plant essential oils (Alpinia galanga, Carum carbi, Cinnamonum zeylanicum, Eugenia caryophyllata, Foeniculum vulgare, Lavandula intermedia cv. Grosso, Melaleuca viridiflora, Mentha pulegium, and Myristica fragrans) were purchased from a local herb market, Skinmate (Bucheon, Korea) and stored in a glass bottle at 4℃. Triton X-100 was supplied by Fluka (Buchs, Switzerland).
The identified compounds in A. galanga oil were methyl cinnamate (87.39%), 1,8-cineole (4.37%), β-pinene (2.54%), α-pinene (2.23%), p-cymene (1.05%), and fenchone (0.53%).
데이터처리
Data were analyzed statistically by analysis of variance and means compared with Duncan’s Multiple Range test (P<0.05).
성능/효과
1). A. galanga was also effective at hatching inhibition, followed by C. zeylanicum, E. caryophyllata, F. vulgare, M. pulegium, and C. carbi. These results indicate that toxicity differences were related to that of susceptibility varies with the developmental stage.
Based on the mortality of juveniles at a concentration of 125 μg/mL, the most active essential oil was A. galanga (100%), followed by C. carbi (22.3%), E. caryophyllata (9.4%), C. zeylanicum (7.2%), M. pulegium (2.4%), and F. vulgare (2.1%).
후속연구
Therefore, natural nematicides based on plant extracts, essential oils, and phytochemicals could be beneficial as alternatives to pesticides for control nematodes. Further experiments are needed to evaluate the nematicidal efficacy under greenhouse and field conditions for the practical use of A. galanga rhizome-derived oil and its volatile constituents. Additionally, nematicidal constituents derived from A.
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