[논문]Triglyceride-Lowering Effects of Two Probiotics, Lactobacillus plantarum KY1032 and Lactobacillus curvatus HY7601, in a Rat Model of High-Fat Diet-Induced Hypertriglyceridemia

Choi, Il-Dong; Kim, Sung-Hwan; Jeong, Ji-Woong; Lee, Dong Eun; Huh, Chul-Sung; Hong, Seong Soo; Sim, Jae-Hun; Ahn, Young-Tae

doi:10.4014/jmb.1512.12018

Triglyceride-Lowering Effects of Two Probiotics, Lactobacillus plantarum KY1032 and Lactobacillus curvatus HY7601, in a Rat Model of High-Fat Diet-Induced Hypertriglyceridemia 원문보기

Journal of microbiology and biotechnology, v.26 no.3, 2016년, pp.483 - 487

Choi, Il-Dong (Korea Yakult Co., Ltd.) , Kim, Sung-Hwan (Korea Yakult Co., Ltd.) , Jeong, Ji-Woong (Korea Yakult Co., Ltd.) , Lee, Dong Eun (Korea Yakult Co., Ltd.) , Huh, Chul-Sung (Graduate School of International Agricultural Technology, Institute of Green Bio Science & Technology, Seoul National University) , Hong, Seong Soo (Division of Gastroenterology, Vievis NAMUH Hospital) , Sim, Jae-Hun (Korea Yakult Co., Ltd.) , Ahn, Young-Tae (Korea Yakult Co., Ltd.)

Abstract ▼ AI-Helper

The triglyceride-lowering effect of probiotics Lactobacillus plantarum KY1032 and Lactobacillus curvatus HY7601 were investigated. Male SD Wistar rats were randomly divided into three groups and fed high-fat diet (HFD), HFD and probiotics (5 × 109 CFU/day of L. plantarum KY1032 and 5 × 109 CFU/day of L. curvatus HY7601), or normal diet for 6 weeks. Probiotic treatment significantly lowered the elevated plasma triglyceride and increased plasma free fatty acid, glycerol, and plasma apolipoprotein A-V (ApoA-V) levels. The probiotic-treated group showed elevated hepatic mRNA expression of PPARα, bile acid receptor (FXR), and ApoA-V. These results demonstrate that L. plantarum KY1032 and L. curvatus HY7601 lower triglycerides in hypertriglyceridemic rats by upregulating ApoA-V, PPARα, and FXR.

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문제 정의

The aim of this study was to determine the TG-lowering effect of naturally derived probiotic strains L. curvatusHY7601 and L. plantarum KY1032 in an HFD (containing 40% beef tallow)-induced hypertriglyceridemia model. Furthermore the relationship between the TG level and the expression of ApoA-V, an important determinant of plasma TG levels, was elucidated.

제안 방법

All rats were individually housed at a constant temperature (22^oC ± 2^oC) and humidity (55% ± 10%) with a 12 h light/dark cycle. After 1 week adaptation, rats were randomly assigned to two groups and fed an HFD (n=20) to induce hypertriglyceridemia or a normal diet (ND group, n=10) for 6 weeks. Of the HFD-fed rats, some were randomly assigned to receive probiotics (HFD + probiotic group, n = 10) or PBS (HFD group, n = 10) for the experimental period.
In this study, we investigated the TG-lowering effect of two probiotic strains, L. plantarum KY1032 and L. curvatus HY7601, in HFD-induced hypertriglyceridemic rats. Our findings showed that these probiotics exert their TG- lowering effects by upregulating the expression of ApoA- V, PPAR_α, and FXR.

이론/모형

RNA expression was quantified by real-time quantitative PCR using the Quanti-Tect SYBR Green RT-qPCR kit (Qiagen). GAPDH was used as an internal control, and relative gene expression was calculated using the 2-DDCt method. As shown in Fig.

성능/효과

In a previous study [2], HFD promoted a significant increase in serum TG concentration over the 7-week experimental period. Therefore, in this study, at the end of the experiment, the plasma levels of total cholesterol (TC), high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and triglyceride (TG) in each group were measured (Fig. 2). Plasma TG, TC, and HDL-C were enzymatically determined using a commercial kit (Asan Co.
None of the genes showed a significant difference in expression among the groups. These results suggest that L. curvatus and L.plantarum exert their TG-lowering effect via upregulation of ApoA-V, and the increased ApoA-V level is associated with and, most likely, due to increased hepatic PPAR_α and FXR expression.

참고문헌 (26)

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Triglyceride-Lowering Effects of Two Probiotics, Lactobacillus plantarum KY1032 and Lactobacillus curvatus HY7601, in a Rat Model of High-Fat Diet-Induced Hypertriglyceridemia 원문보기

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Triglyceride-Lowering Effects of Two Probiotics, Lactobacillus plantarum KY1032 and Lactobacillus curvatus HY7601, in a Rat Model of High-Fat Diet-Induced Hypertriglyceridemia 원문보기

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