[논문]Effects of acetaminophen administration on liver histopathology, serum GOT/GPT levels and circulating microRNA-122 concentration in olive flounder (Paralichthys olivaceus)

Najib, Abdellaoui; Kim, Dong Soo; Kim, Ki Hong

doi:10.7847/jfp.2016.29.1.025

[국내논문] Effects of acetaminophen administration on liver histopathology, serum GOT/GPT levels and circulating microRNA-122 concentration in olive flounder (Paralichthys olivaceus) 원문보기

韓國魚病學會誌= Journal of fish pathology, v.29 no.1, 2016년, pp.25 - 33

Najib, Abdellaoui (Department of Aquatic Life Medicine, Pukyong National University) , Kim, Dong Soo (Department of Aquatic Life Medicine, Pukyong National University) , Kim, Ki Hong (Department of Aquatic Life Medicine, Pukyong National University)

Abstract ▼ AI-Helper

In human medicine, circulating microRNAs have been successfully utilized as early biomarkers for various abnormalities and disease states. Vertebrate miR-122 is a liver-specific microRNA which is expressed almost solely in hepatocytes and plays an important role in the regulation of hepatocyte function. In this study, to evaluate the potential utility of circulating miR-122 as a biomarker for liver injury in olive flounder (Paralichthys olivaceus), fish were orally intubated with two doses of acetaminophen (500 mg/kg or 1.000 mg/kg of body weight), and the expression of miR-122 in serum was quantified using real time-PCR. Histological change in liver, and the enzymatic activity of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) were also analyzed. The results showed that miR-122 was higher in acetaminophen administered groups compared to control group. The histopathological effect of acetaminophen on olive flounder liver was not distinct. The serum level of GPT and GOT was increased within 2 folds compared to control group by acetaminophen administration. However, the serum miR-122 level was increased more than 3 or 4 folds compared to the control group by administration of 1000 mg/kg of acetaminophen. These results suggest the possible use of miR-122 as an indicator of liver injury in olive flounder, even when histopathological effects are not distinctive.

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대상 데이터

Forty five individuals of olive flounder weighing approximately 100 g were obtained from a local farm, and placed in three separate 500 l tanks (15 fish in each tank). After being acclimatized for one week, the control group was orally administrated 100 μl of phosphate buffered saline (PBS).
The treatment groups were administrated acetaminophen (Sigma) per os at a dose of 500 mg/kg and 1000 mg/kg, respectively, using intubation tubes. Blood samples and liver samples were collected at 12, 24, and 48 h post-administration of acetaminophen from randomly sampled 5 fish in each group.

데이터처리

Statistical analysis was performed using SPSS v 20. Differences among groups were analyzed using ANOVA followed by Tukey HSD post-hoc test. P<0.

이론/모형

The blood collected from the caudal vein was kept at room temperature for one hour, centrifuged at 8000 rpm for 10 min at 4℃, and stored at -80℃. Levels of GOT and GPT were detected using ASAN kit (Asan Pharm., Korea) based on Reitman-Frankel method (Reitman, Frankel, 1957).

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