Fusarium graminearum is the main cause of substantial economic loss in wheat production. The aim of this study is to investigate biocontrol potential of Lysobacter antibioticus HS124 against F. graminearum and to purify an antifungal compound. In preliminary study, n-butanol crude extract revealed d...
Fusarium graminearum is the main cause of substantial economic loss in wheat production. The aim of this study is to investigate biocontrol potential of Lysobacter antibioticus HS124 against F. graminearum and to purify an antifungal compound. In preliminary study, n-butanol crude extract revealed destructive alterations in the hyphal morphology of F. graminearum and almost degraded with $1,000{\mu}g\;mL^{-1}$ concentration. For further study, the antifungal compound extracted from the n-butanol crude extract of L. antibioticus HS124 was identified as N-Butyl-tetrahydro-5-oxofuran-2-carboxamide ($C_9H_{16}NO_3$) using NMR ($^1H-NMR$, $^{13}C-NMR$, $^1H-^1H\;COSY$, HMBC, and HMQC), and HR-ESI-MS analysis. To our knowledge, N-Butyl-tetrahydro-5-oxofuran-2-carboxamide may be a novel compound with molecular weight of 186.1130. The minimum inhibitory concentration value of antifungal compound was $62.5{\mu}g\;mL^{-1}$ against F. graminearum. In an in vivo pot experiment, crown rot disease from F. graminearum was inhibited when wheat seeds were treated with both HS124 culture and F. graminearum. Growth of wheat seedling was enhanced by treatment of HS124 compared to control. Our results suggest that L. antibioticus HS124 characterized in this study could be successfully used to control F. graminearum and could be used as an alternative to chemical fungicides in modern agriculture.
Fusarium graminearum is the main cause of substantial economic loss in wheat production. The aim of this study is to investigate biocontrol potential of Lysobacter antibioticus HS124 against F. graminearum and to purify an antifungal compound. In preliminary study, n-butanol crude extract revealed destructive alterations in the hyphal morphology of F. graminearum and almost degraded with $1,000{\mu}g\;mL^{-1}$ concentration. For further study, the antifungal compound extracted from the n-butanol crude extract of L. antibioticus HS124 was identified as N-Butyl-tetrahydro-5-oxofuran-2-carboxamide ($C_9H_{16}NO_3$) using NMR ($^1H-NMR$, $^{13}C-NMR$, $^1H-^1H\;COSY$, HMBC, and HMQC), and HR-ESI-MS analysis. To our knowledge, N-Butyl-tetrahydro-5-oxofuran-2-carboxamide may be a novel compound with molecular weight of 186.1130. The minimum inhibitory concentration value of antifungal compound was $62.5{\mu}g\;mL^{-1}$ against F. graminearum. In an in vivo pot experiment, crown rot disease from F. graminearum was inhibited when wheat seeds were treated with both HS124 culture and F. graminearum. Growth of wheat seedling was enhanced by treatment of HS124 compared to control. Our results suggest that L. antibioticus HS124 characterized in this study could be successfully used to control F. graminearum and could be used as an alternative to chemical fungicides in modern agriculture.
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제안 방법
For the further studies such as minimum inhibitory concentration (MIC) assay and seed treatment in in vivo pot experiments, the spore suspension of F. graminearum was prepared with culturing it with carboxymethyl cellulose broth at 25ºC in shaking incubator for 10 days followed by filtering through cheesecloth.
In disease management, the HS124 may be used as a biological control agent, providing an alternative to the use of chemical synthetic pesticides. The objectives of the current study were to isolate an antifungal compound from L. antibioticus HS124, to characterize its antagonistic activity against F. graminearum, and to investigate the potential of L. antibioticus HS124 to control crown rot of wheat plants in in vivo pot experiment.
graminearum and the crude extract was incubated at 25ºC for 72 h and mycelia were observed under the light microscope (Olympus BX41TF, Japan).All tests were done in triplicate for morphological observation of mycelia.
Evaluation of seeds treated with HS124 for control of Fusarium crown rot of wheat To investigate the ability of HS124 to control Fusarium crown rot (FCR) of wheat in vivo, a pot experiment was conducted. Wheat seeds were disinfected with 1% Sodium hypochlorite (NaOCl) for 1 min and then washed in sterilized distilled water for 3 times.
이론/모형
Determination of MIC The purified compound was tested for MIC values against F. graminearum using broth microdilution technique. A stock solution of 2000 µg mL-1of the purified compound was prepared in methanol.
Determination of MIC MIC value of the purified compound against F. graminearum was determined using a serial dilution method. As shown in Fig.
성능/효과
The result of MIC evaluation demonstrated that the N-Butyl-tetrahydro-5-oxofuran-2-carboxamide had the MIC value of 62.5 μg mL-1against F. graminearum(Fig. 5G).
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