An antimicrobial bacterium to pathogenic microorganisms, strain $W5-1^T$ was isolated from Korean fermented-food Kimchi. The isolate was Gram-staining-variable, strictly aerobic, rod-shaped, endospore-forming, and motile with peritrichous flagella. It grew at $15-40^{\circ}C$, ...
An antimicrobial bacterium to pathogenic microorganisms, strain $W5-1^T$ was isolated from Korean fermented-food Kimchi. The isolate was Gram-staining-variable, strictly aerobic, rod-shaped, endospore-forming, and motile with peritrichous flagella. It grew at $15-40^{\circ}C$, at pH 6.0-10.0, and in the presence of 0-4% NaCl. Strain $W5-1^T$ could hydrolyze esculin and xylan, and assimilate $\small{D}$-mannose, but not $\small{D}$-mannitol. Strain $W5-1^T$ showed antimicrobial activity against Listeria monocytogens, Pseudomonas aeruginosa, Staphylococcus aureus, and Salmonella typhi. The G+C content of the DNA of strains $W5-1^T$ was 52.6 mol%. The predominant respiratory quinone was menaquinone-7 (MK-7) and the major cellular fatty acids were $C_{16:0}$, antieiso-$C_{15:0}$, $C_{18:0}$, and $C_{12:0}$. The strain contained meso-diaminopimelic acid in cell-wall peptidoglycan. On the basis of 16S rRNA gene sequence and phylogenetic analysis, the strain W5-1 was shown to belong to the family Paenibacillaceae and was most closely related to Paenibacillus pinihumi $S23^T$ (98.4% similarity) and Paenibacillus tarimensis $SA-7-6^T$ (96.4%). The DNA-DNA relatedness between the isolate and Paenibacillus pinihumi $S23^T$ was 8.5%, indicating that strain $W5-1^T$ represented a species in the genus Paenibacillus. On the basis of the evidence from this polyphasic study, it is proposed that strain $W5-1^T$ is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus kimchicus sp. nov. is proposed. The type strain is $W5-1^T$ (=KACC $15046^T$ = $LMG 25970^T$).
An antimicrobial bacterium to pathogenic microorganisms, strain $W5-1^T$ was isolated from Korean fermented-food Kimchi. The isolate was Gram-staining-variable, strictly aerobic, rod-shaped, endospore-forming, and motile with peritrichous flagella. It grew at $15-40^{\circ}C$, at pH 6.0-10.0, and in the presence of 0-4% NaCl. Strain $W5-1^T$ could hydrolyze esculin and xylan, and assimilate $\small{D}$-mannose, but not $\small{D}$-mannitol. Strain $W5-1^T$ showed antimicrobial activity against Listeria monocytogens, Pseudomonas aeruginosa, Staphylococcus aureus, and Salmonella typhi. The G+C content of the DNA of strains $W5-1^T$ was 52.6 mol%. The predominant respiratory quinone was menaquinone-7 (MK-7) and the major cellular fatty acids were $C_{16:0}$, antieiso-$C_{15:0}$, $C_{18:0}$, and $C_{12:0}$. The strain contained meso-diaminopimelic acid in cell-wall peptidoglycan. On the basis of 16S rRNA gene sequence and phylogenetic analysis, the strain W5-1 was shown to belong to the family Paenibacillaceae and was most closely related to Paenibacillus pinihumi $S23^T$ (98.4% similarity) and Paenibacillus tarimensis $SA-7-6^T$ (96.4%). The DNA-DNA relatedness between the isolate and Paenibacillus pinihumi $S23^T$ was 8.5%, indicating that strain $W5-1^T$ represented a species in the genus Paenibacillus. On the basis of the evidence from this polyphasic study, it is proposed that strain $W5-1^T$ is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus kimchicus sp. nov. is proposed. The type strain is $W5-1^T$ (=KACC $15046^T$ = $LMG 25970^T$).
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제안 방법
Acid production from glucose, single-carbon-source assimilations and additional physiological characteristics were determined using the API 20NE and API ID32GN galleries (bioMérieux).
Salt tolerance was evaluated after incubation at 30℃ for 7 days in nutrient broth (NB; Difco) containing 0–5% (w/v; interval of 1%) NaCl. The temperature range for growth was assessed on NA incubated for 7 days at 0, 4, 10, 15, 20, 25, 30, 37, 40, 45, and 50℃. The pH range for growth was tested in NB adjusted with HCl and NaOH to pH values between 5.
균주는 15–40℃, pH 6.0– 10.0, 0–4% NaCl 조건에서 생육하였다.
대상 데이터
The 16S rRNA gene was amplified from a single colony by PCR with 2X DyeMIX DNA Polymerase (EnzynomicsTM) and universal bacterial primer pair, 27F (5'-AGAGTTTGATCMT GGCTCAG-3') and 1492R (5'-TACGGYTACCTTGTTACG ACTT-3').
이론/모형
The cellular fatty acids were saponified, methylated, and extracted according to the protocol of the Sherlock Microbial Identification System (MIDI) by using the TSBA 40 method. Extracts were then analyzed by gas chromatograph (Hewlett Packard 6890N) using the Microbial Identification software package (Sasser, 1990). Isoprenoid quinones were extracted with chloroform/methanol (2:1, v/v), evaporated under vacuum conditions, and reextracted in n-hexane-water (1:1, v/v).
and other type strains of the genus Paenibacillus were cultivated for 3 days on TSA. The cellular fatty acids were saponified, methylated, and extracted according to the protocol of the Sherlock Microbial Identification System (MIDI) by using the TSBA 40 method. Extracts were then analyzed by gas chromatograph (Hewlett Packard 6890N) using the Microbial Identification software package (Sasser, 1990).
Gaps were edited in the BioEdit program (Hall, 1999). The evolutionary distances were calculated using the Kimura two-parameter model (Kimura, 1980). The phylogenic tree was constructed by using a neighbor-joining method (Saitou and Nei, 1987), maximum parsimony (Kluge and Farris, 1969) and maximum likelihood (Felsenstein, 1981) in MEGA7 Program (Kumar et al.
성능/효과
균주는 세포벽 펩티도클리칸으로 meso-diaminopimelic acid을 함유하였다. 16S rRNA 유전자서열 분석에 근거하여 W5-1T 균주는 Paenibacillaceae 과로 분류되었으며 Paenibacillus pinihumi S23T (98.4% similarity), P. tarimensis SA-7-6T (96.4%) 균주와 높은 연관성을 보였다. 분리주와 P.
4%. Strain W5-1T showed the highest similarity to P. pinihumi KACC 14199T (98.4%) and the second highest similarities was 96.4% to Paenibacillus tarimensis KACC 14087T. Phylogenetic analyses based on the neighbour-joining, maximum parsimony and maximum-likelihood method revealed that strain W5-1T fell within the evolutionary radiation enclosed by the genus Paenibacillus and formed a cluster with P.
The 16S rRNA gene sequence similarity values between strain W5-1T and the other related type strains of the genus Paenibacillus ranged from 91.5– 98.4%.
균주는 esculin과 xylan을 가수분해하였고, D-mannose을 동화하였으나 D-mannitol 은 동화하지 못하였다. W5-1T 균주는 Listeria monocytogens, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhi에 항균활성을 보였다. W5-1T 균주의 DNA의 G+C 함량 은 52.
4%) 균주와 높은 연관성을 보였다. 분리주와 P. pinihumi S23T 는 8.5%의 DNA-DNA 관련성을 보임으로 W5-1T 균주가 Paenibacillus 속의 한 종임을 보여주었다. 이러한 다각적 연구의 증거로 볼 때 W5-1T 균주는 Paenibacillus 속의 신종으로 사료되어 Paenibacillus kimchicus로 명명을 제안하며, 표준균주는 W5-1T (=KACC 15046T =LMG 25970T)이다.
균주가 한국의 발효식품인 김치에서 분리되었다. 이 분리주는 그람염 색변이성, 절대호기성, 간균, 내생포자형성과 주모성의 편모 를 가지고 운동성을 나타내었다. 균주는 15–40℃, pH 6.
5%의 DNA-DNA 관련성을 보임으로 W5-1T 균주가 Paenibacillus 속의 한 종임을 보여주었다. 이러한 다각적 연구의 증거로 볼 때 W5-1T 균주는 Paenibacillus 속의 신종으로 사료되어 Paenibacillus kimchicus로 명명을 제안하며, 표준균주는 W5-1T (=KACC 15046T =LMG 25970T)이다.
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