[논문]Amycolatopsis sp. KCTC 29142로부터 유래된 siderochelin A의 다제 내성 균주에 대한 항균활성

이동령; 성금화; 이성권; 홍희전; 송재경; 양승환; 서주원

doi:10.7845/kjm.2016.6033

Amycolatopsis sp. KCTC 29142로부터 유래된 siderochelin A의 다제 내성 균주에 대한 항균활성
Anti-multi drug resistant pathogen activity of siderochelin A, produced by a novel Amycolatopsis sp. KCTC 29142 원문보기

Korean journal of microbiology = 미생물학회지, v.52 no.3, 2016년, pp.327 - 335

이동령 (명지대학교 농생명바이오식의약소재개발사업단) , 성금화 (명지대학교 농생명바이오식의약소재개발사업단) , 이성권 (명지대학교 농생명바이오식의약소재개발사업단) , 홍희전 (옥스퍼드 브룩스대학 생의과학과) , 송재경 (국립농업과학원 농업미생물과) , 양승환 (전남대학교 생명산업공학과) , 서주원 (명지대학교 농생명바이오식의약소재개발사업단)

초록
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본 연구에서는 신규 Amycolatopsis 균주 KCTC 29142를 분리하여 형태학적 관찰, 계통분석 및 화학분류학적 분석 등 다상 분류분석을 통해 분석하였다. KCTC 29142 균주의 에틸아세테이트추출물은 강한 항균활성을 나타났고, 활성물질은 철 이온 킬레이트 물질인 siderochelin A로 동정되었다. 본 연구에서 분리된 siderochelin A는 다제내성균인 Acinetobacter baumanii, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Staphylococcus aureus (VRSA), 및 Escherichia coli (E. coli)에 대해 강한 활성을 보였고, 임상에서 분리된 다제내성균에 대한 MIC를 결정하였다.

Abstract ▼ AI-Helper

A novel Amycolatopsis strain KCTC 29142 was isolated and characterized based on the polyphasic taxonomic analysis including morphological observation, phylogenetic analysis, physiological and chemotaxonomic characteristics. The ethyl acetate extract of strain KCTC 29142 culture broth showed strong antibacterial activity and the active compound was identified as siderochelin A, a ferrous-ion chelating compound. In this study, siderochelin A showed good activity against multi-drug resistant pathogens, including Acinetobacter baumanii, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Staphylococcus aureus (VRSA), and Escherichia coli (E. coli). The minimum inhibitory activity against clinical isolates was also determined.

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제안 방법

KCTC 29142 균주의 에틸아세테이트추출물은 강한 항균활성을 나타났고, 활성물질은 철 이온 킬레이트 물질인 siderochelin A로 동정되었다. 본 연구에서 분리된 siderochelin A는 다제내성균인 Acinetobacter baumanii, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Staphylococcus aureus (VRSA), 및 Escherichia coli (E. coli)에 대해 강한 활성을 보였고, 임상에서 분리된 다제내성균에 대한 MIC를 결정하였다.
본 연구에서는 신규 Amycolatopsis 균주 KCTC 29142를 분리하여 형태학적 관찰, 계통분석 및 화학분류학적 분석 등 다상 분류분석을 통해 분석하였다. KCTC 29142 균주의 에틸아세테이트추출물은 강한 항균활성을 나타났고, 활성물질은 철 이온 킬레이트 물질인 siderochelin A로 동정되었다.

이론/모형

Antibacterial activity was measured by the disc diffusion method. Mueller-Hinton agar medium was mixed with 0.
MIC values of the siderochelin A against clinical isolates were determined by agar dilution method. Siderochelin A was added into the Mueller-Hinton (MH) agar at the concentration ranging from 0.
, 1997). The phylogenetic tree was constructed with neighbor-joining (Saitou and Nei, 1987) and Tajima-Nei (Tajima and Nei, 1984) methods using MEGA 4 software (Tamura et al., 2007). Robustness of individual branches was evaluated by bootstrapping of 1,000 replications.

성능/효과

orientalis IMSNU 20058 are white and brownish yellow. Both A. keratiniphila DSM 44586 and A. decaplanina DSM 44594 showed typical morphology of Amycolatopsis species, and the colors are sand yellow (Fig. 2). Other culture characteristics of strain KCTC 29142 on various media are summarized in Supplementary data Table S1.
In conclusion, strain KCTC 29142 produces siderochelin A, which have antibacterial activities on MRSA and other clinical isolates of MDRPs. Inhibitory activity of siderochelin A against MDRP suggested that siderochelin A can be used as a supplementary agent for the treatment of infections caused by MDRPs.
본 연구에서는 신규 Amycolatopsis 균주 KCTC 29142를 분리하여 형태학적 관찰, 계통분석 및 화학분류학적 분석 등 다상 분류분석을 통해 분석하였다. KCTC 29142 균주의 에틸아세테이트추출물은 강한 항균활성을 나타났고, 활성물질은 철 이온 킬레이트 물질인 siderochelin A로 동정되었다. 본 연구에서 분리된 siderochelin A는 다제내성균인 Acinetobacter baumanii, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Staphylococcus aureus (VRSA), 및 Escherichia coli (E.
Morphology showed difference when compared with the closely related strains on Bennett’s medium: Aerial mycelia (front) and substrate mycelia (back) of KCTC 29142 is white and pink, while those of A. orientalis IMSNU 20058 are white and brownish yellow.
8%). These results also suggested that the strain KCTC 29142 belong to the genus Amycolatopsis but have difference with closely related strains.
Phylogenetic relatedness based on the 16S rRNA gene of KCTC 29142 indicated that the closest relatives were Amycolatopsis keratiniphila subsp. nogabecina DSM 44586^T (98%), A. decaplanina DSM 44594^T (98.3%), and A. orientalis IMSNU 20058^T (97%). The DNA G+C content of strain KCTC 29142 was 73.

후속연구

Different activity may due to the different requirement for iron between pathogens. Accurate mechanism should be investigated in the future study.

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