Morphological Features of Marine Dinoflagellates from Jangmok Harbour in Jinhae Bay, Korea: A Case of 30 Species in the Orders Prorocentrales, Dinophysiales, Gonyaulacales and Gymnodiniales원문보기
Shin, Hyeon Ho
(Library of Marine Samples, Korea Institute of Ocean Science and Technology)
,
Kim, Eun Song
(Library of Marine Samples, Korea Institute of Ocean Science and Technology)
,
Li, Zhun
(Library of Marine Samples, Korea Institute of Ocean Science and Technology)
,
Youn, Joo Yeon
(Library of Marine Samples, Korea Institute of Ocean Science and Technology)
,
Jeon, Seul Gi
(Library of Marine Samples, Korea Institute of Ocean Science and Technology)
,
Oh, Seok Jin
(Pukyung National University)
Most previous studies on dinoflagellates in Korean coastal areas were conducted without morphological descriptions and illustrations of the observed dinoflagellates. This indicates that the species and diversity of dinoflagellates may have been respectively misidentified and underestimated in the pa...
Most previous studies on dinoflagellates in Korean coastal areas were conducted without morphological descriptions and illustrations of the observed dinoflagellates. This indicates that the species and diversity of dinoflagellates may have been respectively misidentified and underestimated in the past, probably due to cell shrinkage, distortion and loss caused by sample fixation. This study provides information on the morphological observations of four dinoflagellate orders (Prorocentrales, Dinophysiales, Gonyaulacales and Gymnodiniales) from Jangmok Harbour in Jinhae Bay, Korea. The unfixed samples were collected weekly from December 2013 to February 2015. A total of 13 genera and 30 species were identified using light and scanning electron microscopy, although some samples were not clarified at the species level. Harmful dinoflagellates, Prorocentrum donghaiense, Tripos furca, Alexandrium affine, A. fundyense, Akashiwo sanguinea and Cochlodinium polykrikoides, were identified based on the morphological observations. The results also reflect the occurrence and identification of dinoflagellates that had not been previously recorded in Jangmok Harbour.
Most previous studies on dinoflagellates in Korean coastal areas were conducted without morphological descriptions and illustrations of the observed dinoflagellates. This indicates that the species and diversity of dinoflagellates may have been respectively misidentified and underestimated in the past, probably due to cell shrinkage, distortion and loss caused by sample fixation. This study provides information on the morphological observations of four dinoflagellate orders (Prorocentrales, Dinophysiales, Gonyaulacales and Gymnodiniales) from Jangmok Harbour in Jinhae Bay, Korea. The unfixed samples were collected weekly from December 2013 to February 2015. A total of 13 genera and 30 species were identified using light and scanning electron microscopy, although some samples were not clarified at the species level. Harmful dinoflagellates, Prorocentrum donghaiense, Tripos furca, Alexandrium affine, A. fundyense, Akashiwo sanguinea and Cochlodinium polykrikoides, were identified based on the morphological observations. The results also reflect the occurrence and identification of dinoflagellates that had not been previously recorded in Jangmok Harbour.
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제안 방법
It is matter of concern that many dinoflagellates are not clearly identifiable by light microscopy. Consequently, further studies are needed for the identification of those and other unidentified species, based on techniques such as SEM or molecular phylogenetic analysis.
One millilitre of the sample was placed in a Sedgwick-Rafter chamber, and the cells were isolated under an inverted microscope (Primo vert, Carl Zeiss, Göttingen, Germany), and then identified and photographed using a Zeiss AxioCam MRc digital camera on an Axio Imager 2 upright microscope (Carl Zeiss, Göttingen, Germany).
대상 데이터
Dinoflagellates were collected weekly from December 2013 to February 2015 in Jangmok Harbour, Jinhae Bay (34°59′39.1″N, 128°40′28.8″E), by using a plankton net with a 20-μm mesh (Fig. 1).
The identified cells were transferred into the individual wells of 96 well tissue plates filled with F/2 culture medium and a 30 ml culture flask by micropipetting using a capillary pipette and maintained at 20℃ and ca 100 μmol photons m-2s-1 cool-white illumination under a 14L : 10D photo-cycle. The dinoflagellates reported in this study, excluding heterotrophic species, were deposited at the Library of Marine Samples, Korea Institute of Ocean Science and Technology.
성능/효과
furca depending on the environmental conditions at Jangmok Harbour. In terms of the morphological characteristics of T. furca, it is differentiated from T. fusus and T. muelleri by its external shape, the number and length of its horns, and by the apical horn of T. fusus being longer than those of T. furca and T. muelleri. These species can probably be identified under a microscope, although the plate patterns for such an identification have not been clearly described.
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