To improve the germination of Spiraea fritschiana seeds for mass propagation, we evaluated the effect of a range of temperatures, cold stratification periods, and gibberellic acid ($GA_3$) treatments on three germination characteristics. Final germination percentage (FGP) increased as the...
To improve the germination of Spiraea fritschiana seeds for mass propagation, we evaluated the effect of a range of temperatures, cold stratification periods, and gibberellic acid ($GA_3$) treatments on three germination characteristics. Final germination percentage (FGP) increased as the temperature for seed germination increased, up to $30^{\circ}C$, while the mean germination time (MGT) and the mean number of days to 30% germination ($T_{30}$) decreased when seeds were germinated at $25-30^{\circ}C$. The optimum germination temperature of S. fritschiana seeds is approximately $30^{\circ}C$ considering FGP, MGT, and $T_{30}$ together. FGP increased with the duration of cold stratification up to a period of 6 weeks, but declined after 8 weeks of cold stratification, as prolonged cold stratification can induce dormancy with a resultant decline in germination. Pretreatment with 6-8 weeks of cold stratification or soaking seeds in distilled water or $500mg{\cdot}L^{-1}$$GA_3$ for 24 h accelerated and increased the germination of S. fritschiana seeds, regardless of temperature. However, further study might be required to evaluate the effect of $GA_3$ concentrations lower than $500mg{\cdot}L^{-1}$ on the promotion of germination in S. fritschiana seeds.
To improve the germination of Spiraea fritschiana seeds for mass propagation, we evaluated the effect of a range of temperatures, cold stratification periods, and gibberellic acid ($GA_3$) treatments on three germination characteristics. Final germination percentage (FGP) increased as the temperature for seed germination increased, up to $30^{\circ}C$, while the mean germination time (MGT) and the mean number of days to 30% germination ($T_{30}$) decreased when seeds were germinated at $25-30^{\circ}C$. The optimum germination temperature of S. fritschiana seeds is approximately $30^{\circ}C$ considering FGP, MGT, and $T_{30}$ together. FGP increased with the duration of cold stratification up to a period of 6 weeks, but declined after 8 weeks of cold stratification, as prolonged cold stratification can induce dormancy with a resultant decline in germination. Pretreatment with 6-8 weeks of cold stratification or soaking seeds in distilled water or $500mg{\cdot}L^{-1}$$GA_3$ for 24 h accelerated and increased the germination of S. fritschiana seeds, regardless of temperature. However, further study might be required to evaluate the effect of $GA_3$ concentrations lower than $500mg{\cdot}L^{-1}$ on the promotion of germination in S. fritschiana seeds.
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제안 방법
Seeds were germinated at constant temperatures of 5, 10, 15, 20, 25, 30, or 35°C in a multi-room incubator (HB-302S-2, Hanbaek Scientific Co., Bucheon, South Korea) and illuminated by fluorescent lamps (FCL32SD/30, Kumho Electric, INC., Seoul, South Korea) with a 12 h photoperiod (20 μmol·m-2·s-1).
Seeds were sufficiently moistened on 9 cm Petri dishes containing two layers of filter paper (Whatman No. 2) and incubated at 5°C for 0, 6, 8, 10, 12, or 14 weeks in the dark.
데이터처리
Data were subjected to ANOVA and Duncan’s multiple range test.
z Average values in a column with a different letter are significantly different (p < 0.05) using Duncan’s multiple range test.
성능/효과
In conclusion, the optimal germination temperature is approximately 30°C for S. fritschiana seeds.
The germination characteristics were examined the final germination percentage (FGP), mean germination time (MGT), and the mean number of days for 30% germination (T30). MGT was calculated as MGT = Σ (ti× ni)/Σ ni, where ti is the time (d) from the beginning of the test and ni is the number of germinated seeds at the recorded time (Ellis and Roberts, 1981).
These results indicate that the optimal temperature for germination of S. fritschiana seeds was about 30°C, although the maximum germination percentage was below 80%.
후속연구
fritschiana seeds regardless of temperature. Further research might be useful to evaluate the effect of low concentrations of GA3 solution, such as 10, 20, 50, or 100 mg·L-1 on the promotion of germination in S. fritschiana seeds. Prolonged cold stratification can induce dormancy and result in a decline in germination.
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