[논문]Proteomic Assessment of the Relevant Factors Affecting Pork Meat Quality Associated with Longissimus dorsi Muscles in Duroc Pigs

Cho, Jin Hyoung; Lee, Ra Ham; Jeon, Young-Joo; Park, Seon-Min; Shin, Jae-Cheon; Kim, Seok-Ho; Jeong, Jin Young; Kang, Hyun-sung; Choi, Nag-Jin; Seo, Kang Seok; Cho, Young Sik; Kim, MinSeok S.; Ko, Sungho; Seo, Jae-Min; Lee, Seung-Youp; Shim, Jung-Hyun; Chae, Jung-Il

doi:10.5713/ajas.16.0050

Proteomic Assessment of the Relevant Factors Affecting Pork Meat Quality Associated with Longissimus dorsi Muscles in Duroc Pigs 원문보기

Asian-Australasian journal of animal sciences, v.29 no.11, 2016년, pp.1653 - 1663

Cho, Jin Hyoung (Department of Dental Pharmacology, School of Dentistry and Institute of Dental Bioscience, BK21 plus, Chonbuk National University) , Lee, Ra Ham (Department of Dental Pharmacology, School of Dentistry and Institute of Dental Bioscience, BK21 plus, Chonbuk National University) , Jeon, Young-Joo (Department of Dental Pharmacology, School of Dentistry and Institute of Dental Bioscience, BK21 plus, Chonbuk National University) , Park, Seon-Min (Pohang Center for Evaluation of Biomaterials) , Shin, Jae-Cheon (Pohang Center for Evaluation of Biomaterials) , Kim, Seok-Ho (Aging Research Institute, Korea Research Institute of Bioscience & BioTechnology) , Jeong, Jin Young (Division of Animal Genomics and Bioinformatics, National Institute of Animal science, Rural Development Administration) , Kang, Hyun-sung (Department of Animal Science and Technology, Sunchon National University) , Choi, Nag-Jin (Department of Animal Science, College of Agricultural and Life Science, Chonbuk National University) , Seo, Kang Seok (Department of Animal Science and Technology, Sunchon National University) , Cho, Young Sik (Department of Pharmacy, Keimyung University) , Kim, MinSeok S. (Departme) , Ko, Sungho , Seo, Jae-Min , Lee, Seung-Youp , Shim, Jung-Hyun , Chae, Jung-Il

Abstract ▼ AI-Helper

Meat quality is a complex trait influenced by many factors, including genetics, nutrition, feeding environment, animal handling, and their interactions. To elucidate relevant factors affecting pork quality associated with oxidative stress and muscle development, we analyzed protein expression in high quality longissimus dorsi muscles (HQLD) and low quality longissimus dorsi muscles (LQLD) from Duroc pigs by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic analysis. Between HQLD (n = 20) and LQLD (n = 20) Duroc pigs, 24 differentially expressed proteins were identified by LC-MS/MS. A total of 10 and 14 proteins were highly expressed in HQLD and LQLD, respectively. The 24 proteins have putative functions in the following seven categories: catalytic activity (31%), ATPase activity (19%), oxidoreductase activity (13%), cytoskeletal protein binding (13%), actin binding (12%), calcium ion binding (6%), and structural constituent of muscle (6%). Silver-stained image analysis revealed significant differential expression of lactate dehydrogenase A (LDHA) between HQLD and LQLD Duroc pigs. LDHA was subjected to in vitro study of myogenesis under oxidative stress conditions and LDH activity assay to verification its role in oxidative stress. No significant difference of mRNA expression level of LDHA was found between normal and oxidative stress condition. However, LDH activity was significantly higher under oxidative stress condition than at normal condition using in vitro model of myogenesis. The highly expressed LDHA was positively correlated with LQLD. Moreover, LDHA activity increased by oxidative stress was reduced by antioxidant resveratrol. This paper emphasizes the importance of differential expression patterns of proteins and their interaction for the development of meat quality traits. Our proteome data provides valuable information on important factors which might aid in the regulation of muscle development and the improvement of meat quality in longissimus dorsi muscles of Duroc pigs under oxidative stress conditions.

주제어

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문제 정의

In this study, we focus on its use in the study of livestock muscle development and meat quality with a focus on the differential expression patterns of proteins and their interactions for the development of meat quality traits.

제안 방법

To investigate the patterns of protein expression and efficiency of myotube formation during myogenesis under oxidative stress condition, undifferentiation C2C12 cells were treated with various concentrations of H2O2(12.5 µM to 1 mM).
The highest and lowest meat grades for pH and water holding capacity were identified from the sample which accounted for 10% (20 heads) of the total population (Table 2). To obtain a comprehensive overview of protein components in HQLD and LQLD from 12 individuals, protein profiles of whole lysate of HQLD and LQLD separated by 8% and 12% SDS-PAGE were assessed by silver-stained image analysis (Figure 1A). Patterns of total protein components in whole lysate of HQLD and LQLD were similar among the individual six groups.

대상 데이터

Mitotic C2C12 mouse myoblasts were obtained from Chonbuk University (Jeonju, Korea). C2C12 were passaged as subconfluent monolayers in growth medium (GM) using Dulbecco’s modified Eagle’s medium (Invitrogen, Grand Island, NY, USA) supplemented with 20% fetal bovine serum, 200 mM L-glutamine, 10 units/mL penicillin, and 10 µg/mL streptomycin.

데이터처리

Student’s t test was performed to evaluate statistical significance (ns, non-significant; *** p<0.0001; mean±standard error of the mean; n = 3).

이론/모형

Relative quantification analysis was performed using the comparative Ct (2 (–ΔΔCT)) method.

성능/효과

In conclusion, our data demonstrated that up- or down- regulation of genes and proteins are involved in muscle development, muscle function, actin organization, oxidative stress, cell proliferation, cell differentiation, and cell growth. In this paper, differential expression patterns of genes and their interaction are found to be important for the development of meat quality traits.

후속연구

Our study provided experimental evidence for RSV as an important regulator to improve meat quality grades in porcine. However, further study is required to determine the relationship between differential expression of genes or proteins and their direct effects on meat quality.

참고문헌 (34)

Bendixen E 2005 The use of proteomics in meat science Meat Sci 71 138 149 22064059

상세보기
Bouzid MA Hammouda O Matran R Robin S Fabre C 2014 Changes in oxidative stress markers and biological markers of muscle injury with aging at rest and in response to an exhaustive exercise PLoS One 9 e90420 24618679
Brocks L Hulsegge B Merkus G 1998 Histochemical characteristics in relation to meat quality properties in the Longissimus Lumborum of fast and lean growing lines of Large White pigs Meat Sci 50 411 420 22061343

상세보기
Fan J Hitosugi T Chung TW Xie J Ge Q Gu TL Polakiewicz RD Chen GZ Boggon TJ Lonial S Khuri FR Kang S Chen J 2011 Tyrosine phosphorylation of lactate dehydrogenase A is important for NADH/NAD(+) redox homeostasis in cancer cells Mol Cell Biol 31 4938 4950 21969607

상세보기
Frisby J Raftery D Kerry JP Diamond D 2005 Development of an autonomous, wireless pH and temperature sensing system for monitoring pig meat quality Meat Sci 70 329 336 22063490

상세보기
Gramolini AO Jasmin BJ 1999 Expression of the utrophin gene during myogenic differentiation Nucleic Acids Res 27 3603 3609 10446253

상세보기
Guo K Wang J Andres V Smith RC Walsh K 1995 MyoD-induced expression of p21 inhibits cyclin-dependent kinase activity upon myocyte terminal differentiation Mol Cell Biol 15 3823 3829 7791789

상세보기
Gurnett CA Desruisseau DM McCall K Choi R Meyer ZI Talerico M Miller SE Ju JS Pestronk A Connolly AM Druley TE Weihl CC Dobbs MB 2010 Myosin binding protein C1: a novel gene for autosomal dominant distal arthrogryposis type 1 Hum Mol Genet 19 1165 1173 20045868

상세보기
Halevy O Novitch BG Spicer DB Skapek SX Rhee J Hannon GJ Beach D Lassar AB 1995 Correlation of terminal cell cycle arrest of skeletal muscle with induction of p21 by MyoD Science 267 1018 1021 7863327

상세보기
Hollung K Veiseth E Jia X Faergestad EM Hildrum KI 2007 Application of proteomics to understand the molecular mechanisms behind meat quality Meat Sci 77 97 104 22061400

상세보기
Hwang IH Park BY Kim JH Cho SH Lee JM 2005 Assessment of postmortem proteolysis by gel-based proteome analysis and its relationship to meat quality traits in pig longissimus Meat Sci 69 79 91 22062642

상세보기
Jia X Hollung K Therkildsen M Hildrum KI Bendixen E 2006 Proteome analysis of early post-mortem changes in two bovine muscle types: M. longissimus dorsi and M. semitendinosis Proteomics 6 936 944 16372268

상세보기
Julve J Escola-Gil JC Marzal-Casacuberta A Ordonez-Llanos J Gonzalez-Sastre F Blanco-Vaca F 2000 Increased production of very-low-density lipoproteins in transgenic mice overexpressing human apolipoprotein A-II and fed with a high-fat diet Biochim Biophys Acta 1488 233 244 11082533
Kanter MM Lesmes GR Kaminsky LA La Ham-Saeger J Nequin ND 1988 Serum creatine kinase and lactate dehydrogenase changes following an eighty kilometer race. Relationship to lipid peroxidation Eur J Appl Physiol Occup Physiol 57 60 63 3342795

상세보기
Kislinger T Gramolini AO Pan Y Rahman K MacLennan DH Emili A 2005 Proteome dynamics during C2C12 myoblast differentiation Mol Cell Proteomics 4 887 901 15824125

상세보기
Lametsch R Bendixen E 2001 Proteome analysis applied to meat science: characterizing postmortem changes in porcine muscle J Agric Food Chem 49 4531 4537 11599984

상세보기
Lametsch R Roepstorff P Bendixen E 2002 Identification of protein degradation during post-mortem storage of pig meat J Agric Food Chem 50 5508 5512 12236671

상세보기
Langen RC Van Der Velden JL Schols AM Kelders MC Wouters EF Janssen-Heininger YM 2004 Tumor necrosis factor-alpha inhibits myogenic differentiation through MyoD protein destabilization FASEB J 18 227 237 14769817

상세보기
Lee EJ Malik A Pokharel S Ahmad S Mir BA Cho KH Kim J Kong JC Lee DM Chung KY Kim SH Choi I 2014 Identification of genes differentially expressed in myogenin knock-down bovine muscle satellite cells during differentiation through RNA sequencing analysis PLoS ONE 9 e92447 24647404

상세보기
Liu QC Zha XH Faralli H Yin H Louis-Jeune C Perdiguero E Pranckeviciene E Munoz-Canoves P Rudnicki MA Brand M Perez-Iratxeta C Dilworth FJ 2012 Comparative expression profiling identifies differential roles for Myogenin and p38alpha MAPK signaling in myogenesis J Mol Cell Biol 4 386 397 22847234
Monin G Lambooy E Klont R 1995 Influence of temperature variation on the metabolism of pig muscle in situ and after excision Meat Sci 40 149 158 22059967

상세보기
Montesano A Luzi L Senesi P Mazzocchi N Terruzzi I 2013 Resveratrol promotes myogenesis and hypertrophy in murine myoblasts J Transl Med 11 310 24330398

상세보기
Morzel M Chambon C Hamelin M Sante-Lhoutellier V Sayd T Monin G 2004 Proteome changes during pork meat ageing following use of two different pre-slaughter handling procedures Meat Sci 67 689 696 22061819

상세보기
Mullen AM Stapleton PC Corcoran D Hamill RM White A 2006 Understanding meat quality through the application of genomic and proteomic approaches Meat Sci 74 3 16 22062712

상세보기
Pette D 2002 The adaptive potential of skeletal muscle fibers Can J Appl Physiol 27 423 448 12442355

상세보기
Picard B Berri C Lefaucheur L Molette C Sayd T Terlouw C 2010 Skeletal muscle proteomics in livestock production Brief Funct Genomics 9 259 278 20308039

상세보기
Qiu H Xu X Fan B Rothschild MF Martin Y Liu B 2010 Investigation of LDHA and COPB1 as candidate genes for muscle development in the MYOD1 region of pig chromosome 2 Mol Biol Rep 37 629 636 19830590

상세보기
Sayd T Morzel M Chambon C Franck M Figwer P Larzul C Le Roy P Monin G Cherel P Laville E 2006 Proteome analysis of the sarcoplasmic fraction of pig semimembranosus muscle: implications on meat color development J Agric Food Chem 54 2732 2737 16569068

상세보기
Spangenburg EE Booth FW 2003 Molecular regulation of individual skeletal muscle fibre types Acta Physiol Scand 178 413 424 12864747

상세보기
Stentz R Bongaerts RJ Gunning AP Gasson M Shearman C 2010 Controlled release of protein from viable Lactococcus lactis cells Appl Environ Microbiol 76 3026 3031 20228099

상세보기
Wheeler TL Cundiff LV Shackelford SD Koohmaraie M 2005 Characterization of biological types of cattle (Cycle VII): Carcass, yield, and longissimus palatability traits J Anim Sci 83 196 207 15583060
Wu RE Huang WC Liao CC Chang YK Kan NW Huang CC 2013 Resveratrol protects against physical fatigue and improves exercise performance in mice Molecules 18 4689 4702 23603951

상세보기
Yan JX Wait R Berkelman T Harry RA Westbrook JA Wheeler CH Dunn MJ 2000 A modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry Electrophoresis 21 3666 3672 11271485

상세보기
Zuo X Echan L Hembach P Tang HY Speicher KD Santoli D Speicher DW 2001 Towards global analysis of mammalian proteomes using sample prefractionation prior to narrow pH range two-dimensional gels and using one-dimensional gels for insoluble and large proteins Electrophoresis 22 1603 1615 11425216

상세보기

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