16S rRNA 분석에 의한 Subsection IV cyanobacteria 균주들의 다계통성 기원의 증거 Evidence for Polyphyletic Origin of the Members of the Subsection IV Cyanobacteria as Determined by 16S rRNA Analysis원문보기
Subsection I과 II의 시아노박테리아 균주들은 단세포성이며, Subsection III의 시아노박테리아 균주들은 섬유상의 다계통성, 이형 사이토시스 형성성 균주들인 반면, Subsections IV와 V는 단일계통성으로 보고되어있다. 본 연구에서 13 균주의 시아노박테리아의 the small subunit rRNA (16S rRNA) 염기서열들이 - Subsection III의 Oscillatoria nigro-viridis PCC7112, Subsection IV에 속하는 Anabaena, Nostoc, Tolypothrix, Calothrix 및 Scytonema속을 포함한 6 균주, Subsection V에 속하는 Hapalosiphon, Fischerella and Chlorogloeopsis 속의 6 균주 - 결정되었다. 결정된 16S rRNA 염기서열을 이용하여 시아노박테리아의 분자계통분석을 수행하였다. 그러나, 16S rRNA의 염기서열 결정을 근거로 한 본 연구의 계통분석결과 Subsection IV는 단일 계통성이 아닌 다계통성이며, 반면 Subsection V는 이전에 보고되어진 것처럼 단일 계통성임을 나타내었다. 또한, 본 연구 결과는 Scytonema속이 이형 사이토시스 형성성 시아노박테리아인 Subsection IV 및 V의 공통 조상일 수 있음을 강력하게 나타낸다. 부가적으로, 본 연구의 분자계통 분석을 통해 Anabaena속은 다계통성으로 계통학적으로 다양한 종들로 구성되어 있음을 나타내고 있다. 본 연구 결과는 Anabaena속이 좀 더 세밀하게 재분류 되어져야 함을 나타낸다.
Subsection I과 II의 시아노박테리아 균주들은 단세포성이며, Subsection III의 시아노박테리아 균주들은 섬유상의 다계통성, 이형 사이토시스 형성성 균주들인 반면, Subsections IV와 V는 단일계통성으로 보고되어있다. 본 연구에서 13 균주의 시아노박테리아의 the small subunit rRNA (16S rRNA) 염기서열들이 - Subsection III의 Oscillatoria nigro-viridis PCC7112, Subsection IV에 속하는 Anabaena, Nostoc, Tolypothrix, Calothrix 및 Scytonema속을 포함한 6 균주, Subsection V에 속하는 Hapalosiphon, Fischerella and Chlorogloeopsis 속의 6 균주 - 결정되었다. 결정된 16S rRNA 염기서열을 이용하여 시아노박테리아의 분자계통분석을 수행하였다. 그러나, 16S rRNA의 염기서열 결정을 근거로 한 본 연구의 계통분석결과 Subsection IV는 단일 계통성이 아닌 다계통성이며, 반면 Subsection V는 이전에 보고되어진 것처럼 단일 계통성임을 나타내었다. 또한, 본 연구 결과는 Scytonema속이 이형 사이토시스 형성성 시아노박테리아인 Subsection IV 및 V의 공통 조상일 수 있음을 강력하게 나타낸다. 부가적으로, 본 연구의 분자계통 분석을 통해 Anabaena속은 다계통성으로 계통학적으로 다양한 종들로 구성되어 있음을 나타내고 있다. 본 연구 결과는 Anabaena속이 좀 더 세밀하게 재분류 되어져야 함을 나타낸다.
Unicellular cyanobacterial strains of Subsections I and II and filamentous cyanobacterial strains of Subsection III have been shown to be polyphyletic, heterocystous strains of Subsections IV and V, both of which were previously reported to be monophyletic. In this study, the small subunit ribosomal...
Unicellular cyanobacterial strains of Subsections I and II and filamentous cyanobacterial strains of Subsection III have been shown to be polyphyletic, heterocystous strains of Subsections IV and V, both of which were previously reported to be monophyletic. In this study, the small subunit ribosomal RNA (16S rRNA) sequences of 13 strains of cyanobacteria - one strain, Oscillatoria nigro-viridis PCC7112, of the Subsection III, 6 strains including genus Anabaena, Nostoc, Tolypothrix, Calothrix and Scytonema of the Subsection IV, and 6 strains including genus Hapalosiphon, Fischerella and Chlorogloeopsis of the Subsection V - were determined. The phylogenetic analysis of cyanobacteria was carried out using the 16S rRNA sequences. The results of the phylogenetic analyses of 16S rRNA sequences, based on Neighbour-joining, maximum-parsimony, and maximum-likelihood methods, indicated that the members of Subsection IV were not monophyletic but polyphyletic. In addition, the phylogenetic results strongly indicated that the genus Scytonema in Subsection IV could be a common ancestor of heterocystous cyanobacteria in Subsection IV and V. Furthermore, the phylogenetic analyses revealed that the genus Anabaena could be phylogenetically diverse and that cyanobacterial strains in Subsection IV might be polyphyletic, whereas those in Subsection V could be monophyletic, as reported before. The results for the genus Anabaena indicate that it should be reclassified.
Unicellular cyanobacterial strains of Subsections I and II and filamentous cyanobacterial strains of Subsection III have been shown to be polyphyletic, heterocystous strains of Subsections IV and V, both of which were previously reported to be monophyletic. In this study, the small subunit ribosomal RNA (16S rRNA) sequences of 13 strains of cyanobacteria - one strain, Oscillatoria nigro-viridis PCC7112, of the Subsection III, 6 strains including genus Anabaena, Nostoc, Tolypothrix, Calothrix and Scytonema of the Subsection IV, and 6 strains including genus Hapalosiphon, Fischerella and Chlorogloeopsis of the Subsection V - were determined. The phylogenetic analysis of cyanobacteria was carried out using the 16S rRNA sequences. The results of the phylogenetic analyses of 16S rRNA sequences, based on Neighbour-joining, maximum-parsimony, and maximum-likelihood methods, indicated that the members of Subsection IV were not monophyletic but polyphyletic. In addition, the phylogenetic results strongly indicated that the genus Scytonema in Subsection IV could be a common ancestor of heterocystous cyanobacteria in Subsection IV and V. Furthermore, the phylogenetic analyses revealed that the genus Anabaena could be phylogenetically diverse and that cyanobacterial strains in Subsection IV might be polyphyletic, whereas those in Subsection V could be monophyletic, as reported before. The results for the genus Anabaena indicate that it should be reclassified.
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제안 방법
81 [20]. 16S rRNA sequences were aligned based on their secondary structures with a selection of cyanobacterial reference se- quences obtained from the DNA Data Bank of Japan (DDBJ), and the alignment was manually corrected.
Primers used for the cycle sequencing were 1R, 320R (5’-CTGCTGCCTCCC GATA-3’), 520F (5’-CAGCAGCCGCGGTAATAC-3’), 704R (5’-TCTACGCATTTCACCGCTAC-3’), 926F (5’-AAACTCAA AGGAATTGACGG-3’), 1100R (5’-GGGTTGCGCTVGTTG- 3’) (V, G or C or A), and 16C. The cycle sequencing reaction was performed with a PCR Thermal CyclerMP (Takara, TP3080, Japan). The sequencing reaction was performed for 5 min at 96℃ at first and consisted of 25 cycles of the follow- ing: 10 sec at 96℃, 10 sec at 52℃, and 2 min at 60℃.
대상 데이터
Kit (Applied Biosystems, USA). Primers used for the cycle sequencing were 1R, 320R (5’-CTGCTGCCTCCC GATA-3’), 520F (5’-CAGCAGCCGCGGTAATAC-3’), 704R (5’-TCTACGCATTTCACCGCTAC-3’), 926F (5’-AAACTCAA AGGAATTGACGG-3’), 1100R (5’-GGGTTGCGCTVGTTG- 3’) (V, G or C or A), and 16C. The cycle sequencing reaction was performed with a PCR Thermal CyclerMP (Takara, TP3080, Japan).
이론/모형
The phylogenetic tree was constructed from the evolu- tionary distance matrix calculated by the neighbour-joining method [19] with Kimura`s two-parameter method [12]. Neighbour-joining analysis was performed using the MEGA2 program [13].
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