Pseudolaric acids of Pseudolarix kaempferi (Pinaceae) have been known as diterpenoids with potent anti-fungal-, anti-microbial, and cytotoxic activities. In the present study, the five MeOH extracts were prepared from the five plant part (root bark, stem bark, leaf, the inner part of root, and cone)...
Pseudolaric acids of Pseudolarix kaempferi (Pinaceae) have been known as diterpenoids with potent anti-fungal-, anti-microbial, and cytotoxic activities. In the present study, the five MeOH extracts were prepared from the five plant part (root bark, stem bark, leaf, the inner part of root, and cone) to find the relation between the concentration of pseudolaric acids and cytotoxicity. Pseudolaric acids B and C were isolated from the root bark of P. kaempferi to use them as standard compounds. The five extracts were tested on cytotoxicity against six cancer cell lines, A549 (lung), HCT116 (colon), MDA-MB-231 (breast), SNU638 (stomach), and SK-hep-1 (liver) by SRB assay, but against K562 (leukemia) by SRB- or MTT assay. HPLC quantification were performed on a Shisheido Capcell PAK C18 column ($5{\mu}m$, $4.6mm{\times}250mm$) using 254 nm wavelength. The cytotoxicity ($IC_{50}$, $0.36{\mu}g/ml$ on K562 cell lines) of the root bark extract was potent and the content (101.1 mg/g extract) of pseudolaric acid B was very high in the root bark. These results suggest that the MeOH extract obtained from the root bark could be developed as the anti-cancer agent with a high quantity of pseudolaric acid B.
Pseudolaric acids of Pseudolarix kaempferi (Pinaceae) have been known as diterpenoids with potent anti-fungal-, anti-microbial, and cytotoxic activities. In the present study, the five MeOH extracts were prepared from the five plant part (root bark, stem bark, leaf, the inner part of root, and cone) to find the relation between the concentration of pseudolaric acids and cytotoxicity. Pseudolaric acids B and C were isolated from the root bark of P. kaempferi to use them as standard compounds. The five extracts were tested on cytotoxicity against six cancer cell lines, A549 (lung), HCT116 (colon), MDA-MB-231 (breast), SNU638 (stomach), and SK-hep-1 (liver) by SRB assay, but against K562 (leukemia) by SRB- or MTT assay. HPLC quantification were performed on a Shisheido Capcell PAK C18 column ($5{\mu}m$, $4.6mm{\times}250mm$) using 254 nm wavelength. The cytotoxicity ($IC_{50}$, $0.36{\mu}g/ml$ on K562 cell lines) of the root bark extract was potent and the content (101.1 mg/g extract) of pseudolaric acid B was very high in the root bark. These results suggest that the MeOH extract obtained from the root bark could be developed as the anti-cancer agent with a high quantity of pseudolaric acid B.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
kaempferi to use it as standard compounds in cytotoxicity assay and in HPLC analysis. After the five extracts were prepared from the root bark, stem bark, leaf, the inner part of root, and cone of this plant, the cytotoxic activities were determined. The content of pseudolaric acids B and C were also comparatively studied using HPLC.
kaempferi was examined by testing the cytotoxicity of the five plant part extracts (MeOH extracts). HPLC quantification was performed to correlate the content of the cytotoxic diterpenoids with their cytotoxicity. This biological activities were assayed by the SRB method against the five cancer cell lines (A549 (lung), HCT116 (colon), MDA-MB-231 (breast), SNU638 (stomach), and SK-hep-1 (liver), but by the MTT assay against K562 (leukemia).
Therefore, the extract comprising potent cytotoxic agents can be also expected as the anti-cancer agent. In this study, pseudolaric acids B and C were isolated from P. kaempferi to use it as standard compounds in cytotoxicity assay and in HPLC analysis. After the five extracts were prepared from the root bark, stem bark, leaf, the inner part of root, and cone of this plant, the cytotoxic activities were determined.
대상 데이터
Plant material − The plant materials of the five plant parts (root bark, stem bark, leaf the inner part of root, and cone) were purchased in an herb store in Wonju.
Plant material − The plant materials of the five plant parts (root bark, stem bark, leaf the inner part of root, and cone) were purchased in an herb store in Wonju. This plant material was identified by Dr. Nam-Tae Woo (Department of Oriental medicine, Sangji University). A voucher specimen (natchem-#122) was deposited in the Laboratory of Natural Products Chemistry, Department of Pharmaceutical Engineering, Sangji University).
데이터처리
The percentage of cell proliferation was determined according to the following formula: cell proliferation (%) = 100 × [(A treated – A zeroday) / (A control – A zero day)], where A is the average absorbance. The IC50 values were calculated through non-linear regression analysis using TableCurve 2D v5.01 (Systat Software Inc., San Jose, CA, USA).
성능/효과
Considering the results of the HPLC chromatogram and the cytotoxicity, pseudolaric acid B may be the final product to resist fungal microorganisms. In conclusion, the root bark extract could be developed as the potential biomaterial for an anticancer agent since its cytotoxic potency was very high against various cancer cell lines.
참고문헌 (11)
Yang, S. P.; Dong, L.; Wang, Y.; Wu, Y.; Yue, J. M. Med. Chem. 2003, 11, 4577-4584.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.