Caryopteris incana (Verbenaceae) has been used to treat cough, arthritis, and eczema in Oriental medicine. The two fractions ($CHCl_3-$ and BuOH fractions) and the essential oil of the plant material were subjected to the inducible nitric oxide synthase (iNOS) assay. The $IC_{50}$
Caryopteris incana (Verbenaceae) has been used to treat cough, arthritis, and eczema in Oriental medicine. The two fractions ($CHCl_3-$ and BuOH fractions) and the essential oil of the plant material were subjected to the inducible nitric oxide synthase (iNOS) assay. The $IC_{50}$ of the $CHCl_3$ fraction and the essential oil on LPS-induced macrophage RAW 264.7 cells were $16.4{\mu}g/mL$ and $23.08{\mu}g/mL$, respectively. On gas chromatography (GC)-mass spectroscopy (MS) analysis, twenty-five components representing 85.5% amount of total essential oil were identified. On the chromatogram, three main substances, trans-pinocarveol, cis-citral, and pinocarvone, occupied 18.8%, 13.5% and 18.37% of total peak area. Furthermore, by HPLC-UV analysis, six compounds including one iridoid (8-O-acetylharpagide)- and five phenylethanoid glycosides (caryopteroside, acteoside, phlinoside A, 6-O-caffeoylphlinoside, and leucosceptoside A) isolated from the BuOH fraction were quantified. The content of six compounds were shown as the following order: caryopteroside (162.35 mg/g) > 8-O-acetylharpagide (93.28 mg/g) > 6-O-caffeoylphlinoside (28.15 mg/g) > phlinoside (22.60 mg/g) > leucosceptoside A (16.87 mg) > acteoside (7.05 mg/g).
Caryopteris incana (Verbenaceae) has been used to treat cough, arthritis, and eczema in Oriental medicine. The two fractions ($CHCl_3-$ and BuOH fractions) and the essential oil of the plant material were subjected to the inducible nitric oxide synthase (iNOS) assay. The $IC_{50}$ of the $CHCl_3$ fraction and the essential oil on LPS-induced macrophage RAW 264.7 cells were $16.4{\mu}g/mL$ and $23.08{\mu}g/mL$, respectively. On gas chromatography (GC)-mass spectroscopy (MS) analysis, twenty-five components representing 85.5% amount of total essential oil were identified. On the chromatogram, three main substances, trans-pinocarveol, cis-citral, and pinocarvone, occupied 18.8%, 13.5% and 18.37% of total peak area. Furthermore, by HPLC-UV analysis, six compounds including one iridoid (8-O-acetylharpagide)- and five phenylethanoid glycosides (caryopteroside, acteoside, phlinoside A, 6-O-caffeoylphlinoside, and leucosceptoside A) isolated from the BuOH fraction were quantified. The content of six compounds were shown as the following order: caryopteroside (162.35 mg/g) > 8-O-acetylharpagide (93.28 mg/g) > 6-O-caffeoylphlinoside (28.15 mg/g) > phlinoside (22.60 mg/g) > leucosceptoside A (16.87 mg) > acteoside (7.05 mg/g).
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제안 방법
0 L to remove sugars or ionic substances. Successive elution was conducted with each 1.0 L solvent of 25% MeOH, 50% MeOH, 75% MeOH, and 100% MeOH, every eluted solution was concentrated to produce a 25% MeOH extract (5.3 g), 50% MeOH extract (1.9 g), 75% MeOH extract (8.3 g), and 100% MeOH (6.2 g) extract.
대상 데이터
Byong-Min Song (Department of Forest Science, Sangji University). A voucher specimen (natchem-#92) was deposited in the Laboratory of Natural Products Sciences, Department of Pharmaceutical Engineering, Sangji University.
Regarding sesquiterpenes, the eleven compounds including α-copaene, γcadinene, γ-2-cadinene, δ-cadinene, β-copaene-4α-ol, αcampholenal, ledol, α-cedrene, τ-muurodol, caryophyllene epoxide, and α-bisabolene epoxide were identified. The 25 compounds identified in this study represented 85.5% quantity of the total essential oil.
Since the essential oil shares volatile substances with the CHCl3 fraction containing less polar substances, this oil was analyzed by GC-MS. Twenty-five components were identified by matching each mass spectrum with the data of MS library. Of compounds 1 – 14, twelve peaks besides compounds 1 and 2 belonged to monoterpenes.
성능/효과
In conclusion, the essential oil obtained from C. incana inhibited NO formation in macrophage 264.7 cells. GCMS analysis of the essential oil led to the identification of 25 volatile substances and the finding of three main substances, trans-pinocarveol (4, 18.
참고문헌 (11)
Moncada, S.; Palmer, R. M. J.; Higgs, E. A. Pharmacol. Rev. 1991, 43,109-142.
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