BACKGROUND/OBJECTIVES: Gastrodia elata Blume (GEB), a traditional herbal medicine, has been used to treat a wide range of neurological disorders (e.g., paralysis and stroke) and skin problems (e.g., atopic dermatitis and eczema) in oriental medicine. This study was designed to investigate whether GE...
BACKGROUND/OBJECTIVES: Gastrodia elata Blume (GEB), a traditional herbal medicine, has been used to treat a wide range of neurological disorders (e.g., paralysis and stroke) and skin problems (e.g., atopic dermatitis and eczema) in oriental medicine. This study was designed to investigate whether GEB extract inhibits melanogenesis activity in murine B16F10 melanoma. MATERIALS/METHOD: Murine B16F10 cells were treated with 0-5 mg/mL of GEB extract or $400{\mu}g/mL$ arbutin (a positive control) for 72 h after treatment with/without 200 nM alpha-melanocyte stimulating hormone (${\alpha}$-MSH) for 24 h. Melanin concentration, tyrosinase activity, mRNA levels, and protein expression of microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein (Trp)1, and Trp2 were analyzed in ${\alpha}$-MSH-untreated and ${\alpha}$-MSH-treated B16F10 cells. RESULTS: Treatment with 200 nM ${\alpha}$-MSH induced almost 2-fold melanin synthesis and tyrosinase activity along with increased mRNA levels and protein expression of MITF, tyrosinase, Trp1 and Trp2. Irrespective of ${\alpha}$-MSH stimulation, GEB extract at doses of 0.5-5 mg/mL inhibited all these markers for skin whitening in a dose-dependent manner. While lower doses (0.5-1 mg/mL) of GEB extract generally had a tendency to decrease melanogenesis, tyrosinase activity, and mRNA levels and protein expression of MITF, tyrosinase, Trp1, and Trp2, higher doses (2-5 mg/mL) significantly inhibited all these markers in ${\alpha}$-MSH-treated B16F10 cells in a dose-dependent manner. These inhibitory effects of the GEB extract at higher concentrations were similar to those of $400{\mu}g/mL$ arbutin, a well-known depigmenting agent. CONCLUSIONS: These results suggest that GEB displays dose-dependent inhibition of melanin synthesis through the suppression of tyrosinase activity as well as molecular levels of MITF, tyrosinase, Trp1, and Trp2 in murine B16F10 melanoma. Therefore, GEB may be an effective and natural skin-whitening agent for application in the cosmetic industry.
BACKGROUND/OBJECTIVES: Gastrodia elata Blume (GEB), a traditional herbal medicine, has been used to treat a wide range of neurological disorders (e.g., paralysis and stroke) and skin problems (e.g., atopic dermatitis and eczema) in oriental medicine. This study was designed to investigate whether GEB extract inhibits melanogenesis activity in murine B16F10 melanoma. MATERIALS/METHOD: Murine B16F10 cells were treated with 0-5 mg/mL of GEB extract or $400{\mu}g/mL$ arbutin (a positive control) for 72 h after treatment with/without 200 nM alpha-melanocyte stimulating hormone (${\alpha}$-MSH) for 24 h. Melanin concentration, tyrosinase activity, mRNA levels, and protein expression of microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein (Trp)1, and Trp2 were analyzed in ${\alpha}$-MSH-untreated and ${\alpha}$-MSH-treated B16F10 cells. RESULTS: Treatment with 200 nM ${\alpha}$-MSH induced almost 2-fold melanin synthesis and tyrosinase activity along with increased mRNA levels and protein expression of MITF, tyrosinase, Trp1 and Trp2. Irrespective of ${\alpha}$-MSH stimulation, GEB extract at doses of 0.5-5 mg/mL inhibited all these markers for skin whitening in a dose-dependent manner. While lower doses (0.5-1 mg/mL) of GEB extract generally had a tendency to decrease melanogenesis, tyrosinase activity, and mRNA levels and protein expression of MITF, tyrosinase, Trp1, and Trp2, higher doses (2-5 mg/mL) significantly inhibited all these markers in ${\alpha}$-MSH-treated B16F10 cells in a dose-dependent manner. These inhibitory effects of the GEB extract at higher concentrations were similar to those of $400{\mu}g/mL$ arbutin, a well-known depigmenting agent. CONCLUSIONS: These results suggest that GEB displays dose-dependent inhibition of melanin synthesis through the suppression of tyrosinase activity as well as molecular levels of MITF, tyrosinase, Trp1, and Trp2 in murine B16F10 melanoma. Therefore, GEB may be an effective and natural skin-whitening agent for application in the cosmetic industry.
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대상 데이터
GEB were obtained from the Hyewonchunma Farm (Chuncheon, Korea). The dried GEB (42°C for 16 h) were ground and then GEB powder (10 g) was extracted in 200 mL of de-ionized H2O at 90°C for 3 h, followed by centrifugation at 4,500 rpm.
Murine B16F10 melanoma line (Korean Cell Line Bank, Seoul, Korea) was cultured in Dulbecco's Modified Eagle Medium (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (Gibco) and 1% penicillin-streptomycin (Gibco) in a 5% CO2 atmosphere.
데이터처리
A one-way analysis of variance (ANOVA) followed by a post-hoc analysis with Tukey's test were used to detect differences between experimental groups.
이론/모형
Each sample was normalized to a total cellular protein content using the Pierce® BCA method (Thermo Scientific).
참고문헌 (36)
1 Chang T Natural melanogenesis inhibitors acting through the down-regulation of tyrosinase activity c 2012 5 1661 1685
2 Chen WC Tseng TS Hsiao NW Lin YL Wen ZH Tsai CC Lee YC Lin HH Tsai KC Discovery of highly potent tyrosinase inhibitor, T1, with significant anti-melanogenesis ability by zebrafish in vivo assay and computational molecular modeling Sci Rep 2015 5 7995 8002 25613357
4 Heilgemeir GP Balda BR Irreversible toxic depigmentation. Observations following use of hydroquinonemonobenzylether-containing skin bleaching preparations MMW Munch Med Wochenschr 1981 123 47 48
5 Mishima Y Hatta S Ohyama Y Inazu M Induction of melanogenesis suppression: cellular pharmacology and mode of differential action Pigment Cell Res 1988 1 367 374 3148920
7 Funayama M Arakawa H Yamamoto R Nishino T Shin T Murao S Effects of α-and β-arbutin on activity of tyrosinases from mushroom and mouse melanoma Biosci Biotechnol Biochem 1995 59 143 144 7765966
8 Park E Lee WY Kim ST Ahn JK Bae EK Ergothioneine accumulation in a medicinal plant Gastrodia elata J Med Plants Res 2010 4 1141 1147
9 Jang JH Son Y Kang SS Bae CS Kim JC Kim SH Shin T Moon C Neuropharmacological potential of Gastrodia elata Blume and its components Evid Based Complement Alternat Med 2015 2015 309261 26543487
10 Chik SC Or TC Luo D Yang CL Lau AS Pharmacological effects of active compounds on neurodegenerative disease with gastrodia and uncaria decoction, a commonly used poststroke decoction ScientificWorldJournal 2013 2013 896873 24348193
11 Kim HT Park EJ Change of major functional components of Gastrodia elata Blume with cultivation conditions and harvest times Korean J Med Crop Sci 2013 21 282 288
12 Kim MH Kim JG Choi JH Antioxidant activity and changes in major functional components of fermented Gastrodia elata Blume Korean J Food Nutr 2014 27 684 691
13 Kim HJ Lee JH Shin MK Hyun Leem K Kim YJ Lee MH Inhibitory effect of Gastrodia elata extract on melanogenesis in HM3KO melanoma cells J Cosmet Sci 2013 64 89 98 23578832
14 Kim KT Kim JG Park SH Lee JH Lee SH Kim KH Park SN Anti-melanogenesis effect of phenolic compounds isolated from Gastrodia elata J Soc Cosmet Sci Korea 2004 30 33 38
15 Chen T Li C Zhu T Wu D Xu W Han H Ffects of gastrodin on formation of B16 melanoma cell melanogenesis J Anhui Agric Sci 2013 5280 5282
16 Liu SH Pan IH Chu IM Inhibitory effect of p-hydroxybenzyl alcohol on tyrosinase activity and melanogenesis Biol Pharm Bull 2007 30 1135 1139 17541167
17 Wang Y Lin S Chen M Jiang B Guo Q Zhu C Wang S Yang Y Shi J Chemical constituents from aqueous extract of Gastrodia elata Zhongguo Zhong Yao Za Zhi 2012 37 1775 1781 22997823
18 Dubost NJ Beelman RB Peterson D Royse DJ Identification and quantification of ergothioneine in cultivated mushrooms by liquid chromatography-mass spectroscopy Int J Med Mushrooms 2006 8 215 222
19 Cheah IK Halliwell B Ergothioneine; antioxidant potential, physiological function and role in disease Biochim Biophys Acta 2012 1822 784 793 22001064
20 Chen Y Tang YM Yu SL Han YW Kou JP Liu BL Yu BY Advances in the pharmacological activities and mechanisms of diosgenin Chin J Nat Med 2015 13 578 587 26253490
21 Cronin H Draelos ZD Top 10 botanical ingredients in 2010 anti-aging creams J Cosmet Dermatol 2010 9 218 225 20883295
22 Shin CS Park CK Lee JW Lee JG Jang CK Kim YK Analysis of the components with freeze drying and steam drying of Gastrodia elata Blume J Korean Soc Food Sci Nutr 1999 28 1058 1063
23 Kim HJ Chung SK Moon KD Chemical components of Gastrodia elata Blume powder Korean J Postharvest Sci Technol 2000 7 278 284
24 Paik YS Song JK Yoon CH Jung KS Yoon HS Anti-platelet and anti-thrombotic effects of Gastrodia elata Korean J Pharmacogn 1995 26 385 389
25 Briganti S Camera E Picardo M Chemical and instrumental approaches to treat hyperpigmentation Pigment Cell Res 2003 16 101 110 12622786
26 Lee KT Kim BJ Kim JH Heo MY Kim HP Biological screening of 100 plant extracts for cosmetic use (I): inhibitory activities of tyrosinase and DOPA auto-oxidation Int J Cosmet Sci 1997 19 291 298 18505483
27 Kim CH Ha BJ Koo CS Chang DI Development of bioconverted skin-whitening cosmetics from Gastrodia elata Theor Appl Chem Eng 2009 15 2512
28 Kim KT Kim JK Park SH Lee JH Lee SH Kim KH Park SN Anti-melanogenesis effect of phenolic compounds isolated from Gastrodia elata J Soc Cosmet Sci Korea 2004 30 33 38
29 Wu SY Wang HM Wen YS Liu W Li PH Chiu CC Chen PC Huang CY Sheu JH Wen ZH 4-(Phenylsulfanyl)butan-2-one suppresses melanin synthesis and melanosome maturation in vitro and in vivo Int J Mol Sci 2015 16 20240 20257 26343635
30 Pei CJ Lee J Si YX Oh S Xu WA Yin SJ Qian GY Han HY Inhibition of tyrosinase by gastrodin: an integrated kinetic-computational simulation analysis Process Biochem 2013 48 162 168
31 Fenoll LG Rodríguez-López JN García-Sevilla F García-Ruiz PA Varón R García-Cánovas F Tudela J Analysis and interpretation of the action mechanism of mushroom tyrosinase on monophenols and diphenols generating highly unstable o-quinones Biochim Biophys Acta 2001 1548 1 22 11451433
32 Bagheri-Kalmarzi M Sajedi RH Asadollahi E Mahmoodi NO Haji-Hosseini R Effect of vanillin and its acid and alcohol derivatives on the diphenolase activity of mushroom tyrosinase Mol Biol Res Commun 2012 1 74 82
33 Liao WC Wu WH Tsai PC Wang HF Liu YH Chan CF Kinetics of ergothioneine inhibition of mushroom tyrosinase Appl Biochem Biotechnol 2012 166 259 267 22068690
34 Lee J Jung K Kim YS Park D Diosgenin inhibits melanogenesis through the activation of phosphatidylinositol-3-kinase pathway (PI3K) signaling Life Sci 2007 81 249 254 17568620
35 Tada Y Kanda N Haratake A Tobiishi M Uchiwa H Watanabe S Novel effects of diosgenin on skin aging Steroids 2009 74 504 511 19428439
36 Song E Chung H Shim E Jeong JK Han BK Choi HJ Hwang J Gastrodia elata Blume extract modulates antioxidant activity and Ultraviolet A-irradiated skin aging in human dermal fibroblast cells J Med Food 2016 19 1057 1064 27845865
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