[논문]도꼬마리 추출물의 산화적 스트레스에 대한 융합연구

민영실

doi:10.15207/jkcs.2017.8.12.215

도꼬마리 추출물의 산화적 스트레스에 대한 융합연구
Convergence study of oxidative stress from fraction of Xanthium strumarium L. 원문보기

한국융합학회논문지 = Journal of the Korea Convergence Society, v.8 no.12, 2017년, pp.215 - 220

민영실 (중원대학교)

초록
AI-Helper

도꼬마리 (X. strumarium )는 국화과에 속하며, '창이자'라는 한약명으로 사용되는 다년생 식물이다. 열매부위와 지상부위의 추출물을 이용하여 융합 식물활성작용을 관찰하였다. 산화적 스트레스에 대항하는 실험법으로는 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH), ABTS assay를 사용하였다. 도꼬마리의 추출물은 단일분리가 아닌 ethanol, petroleum ether, 그리고 ethyl acetate로 추출하였다. 산화적 스트레스연구에 사용되는 radical scavenging 능력을 확인하기 위하여 DPPH radical and ABTS를 투여하였다. 본 연구에서 도꼬마리는 천연유래 항산화 효과를 보였다.

Abstract ▼ AI-Helper

Xanthium strumarium L. is an annual plant belongs to the family Asteraceae which is is called a 'Cocklebur' that is used for medicinal purposes. Convergent phyto-activity of various extracts of Xanthium strumarium L. (Asteraceae) was examined. We estimated antioxidant activity from ground part and fruit extract of X. strumarium using 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH) and ABTS assay. The extract of X. strumarium was separated each fraction that of ethanol, petroleum ether, and ethyl acetate. It showed potent radical scavenging effect against the DPPH radical and ABTS. The study revealed that X. strumarium could be used as a potential source of natural antioxidant.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

This study reveals that the X. strumarium could be used as a potential source of natural antioxidants.

가설 설정

E.A fraction of X. strumarium ground part showed the strongest effects. The IC₅₀ was measured at 4.
That is measured at 732nm using spectrophotometer[4]. This study, E.A fraction of X. strumarium fruit and P.E fraction of X. strumarium, IC₅₀ value is lower than gallic acid. But the reise enough value as a natural anti-oxidant.

제안 방법

After isolate the ground part and fruit of Xanthium strumarium L., each extract was separated and measured radical scavenging activity of DPPH and ABTS to evaluate the antioxidant activity of them. DPPH assay is founded on the measurement of the scavenging activity of antioxidant.

대상 데이터

The plant was collected at Goesan-gun, Chungcheongbuk-do in Korea. The leaves were freeze-dried (FD5508, Ilsin BioBase, Korea) for two weeks and grounded to powder using pestle and mortar.

데이터처리

The statistical significance between antioxidant activity values of the extracts was evaluated with Student’s t - test.

성능/효과

Our results showed potent radical scavenging effect against the DPPH radical IC50, ground part of X. strumarium P.E 9.61 ± 1.20 μg/ml, fruits part of X. strumarium P.E 8.03 ± 0.10 μg/ml, respectively.
The results of our study could be suggested that P.E fraction of X. strumarium fruit and P.E fraction of the ground part have antioxidant activity. It could be used as a potential source of natural antioxidant due to it has antioxidant ability.

참고문헌 (22)

J. H. Park, Y .S. Min. "Antioxidant Activities of Xanthium strumarium Fruit and stem Extracts." The Journal of Applied Oriental Medicine, Vol. 10, No. 2, pp. 17-23, 2010.
A. Sanchez-Lamar, J, Piloto-Ferrer. F. Degrassi. "Xanthium strumarium extract inhibits mammalian cell proliferation through mitotic spindle disruption mediated by xanthatin." J Ethnopharmacol, Vol. 194, pp. 781-788, 2016.

상세보기
C. S. Yook. Clourred medicinal plants of Korea, Seoul Academy press pp. 305-310, 1990.
K.W. Lee, S. J. Lee, H. K. Park, M. J. Kim, J. S. Jeong, E. K. Hong, C. K. Joo. "Antioxidant Activity of Xanthium Strumarium L. Extracted Fractions in Lens Epithelial Cells." Journal of The Korean Ophthalmological Society, Vol 42, No 1, pp. 152-159, 2001.
NB. Sridlharamurthy, R. Yogananda, U. Srinivas. "In-vitro antioxidant and Antilipidemic Activites of Xanthium." Current Trends in Biotechnology & Pharmacy, Vol. 5, No. 3, pp. 1362-1371, 2011.
D Jain, SB Shukla, S Verma "Lack of analgesic property in Xantium strumarium Linn. Fruit's butanolic extracts." Journal of Pharmaceutical Negative Results Vol. 3, No. 1, pp. 22-25, 2012.
K. Apel, H. Hirt. "Reactive Oxygen Species: Metabolism, Oxidative Stress, and Signal Transduction." Annu. Rev. Plant Biol. Vol. 55, pp. 373-399, 2004.

상세보기
M. Al Shahrani, S. Heales, I. Hargreaves, M Orford. "Oxidative Stress: Mechanistic Insights into Inherited Mitochondrial Disorders and Parkinson's Disease." Clin, Med., vol. 6, No. 11, p. 100. 2017.

상세보기
JT. Kumpulainen, JT. Salonen. "Natural Antioxidants and Anticarcinogenens in Nutrition, Health and Disease." The Royal Society of chemistry, UK, pp. 69-83. (1999).
F. Pourmorad, SJ. Hosseinimehr, N. Shahabimajd. "Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants." African Journal of Biotechnology, Vol. 5, No. 11, pp. 1142-1145, 2006.
JM Kim, SH. Choi, OH. Lee. "Method validation and measurement uncertainty for the simultaneous determination of synthetic phenolic antioxidants in edible oils commonly consumed in Korea." Food Chem., Vol. 213, No. 15, pp. 19-25, 2016.

상세보기
S. Robu, AC Aprotosoaie AC, A. Miron, O. Cioanca, "In vitro antioxidant activity of Ethanolic extracts from some Lavandula species cultivated in Romania." Farmacia, Vol. 60, No. 3, pp. 394-401, 2012.

상세보기
N. Gupta, S. Bhattacharya, RK Sharma, E. Narang. "In vitro Comparative Antioxidant Activity of Ethanolic Extracts of Glycosmis pentaphylla, Bauhinia variegata and Leucas aspera." Journal of Pharmaceutical Research, Vol. 10, No. 1, pp. 42-45. 2011.
H.S. Kim, I. S. Lee, S. H. Yeo, L. S. Seong, T. S. Yu, "Isolization and characterization of antitumor agents from Xanthium strumarium L." Korean J. Biotechnol. Bioeng, Vol. 18, No. 4, pp. 324-328, 2003.
S. M. Park, H. J. Jung, S. H. Han, S. H. Yeo, Y. W. Kim, H. G. Ahn, H. S. KimS, T. S. Yu. "Antifungal activity of extract from Xanthium strumarium L. against plant pathogenous fungi." Journal of Life Science, Vol. 15, No. 5, pp. 692-695, 2005.

원문보기 상세보기
B. Hsu, L. M. Coupar, K. Ng. "Antioxidant activity of hot water extract from the fruit of the Doum palm, Hyphaene thebaica." Food Chem, Vol. 98, No. 2. pp. 317-328, 2006.

상세보기
Y. M. Park, J. B. Jeong, J. H. Seo, J. H. Lim, H. J. Jeong, E. W. Seo. "Inhibitory Effect of Red Bean (Phaseolus angularis) Hot Water Extracts on Oxidative DNA and Cell Damage.", Korean J. Plant Res, Vol. 24, No. 2, pp. 130-138, 2011.

원문보기 상세보기
M. Nasir, S. Rauf, A. Hayat. "Biomimetic nitrogen doped titania nanoparticles as a colorimetric platform for hydrogen peroxide detection." J. Colloid Interface Sci. Vol 505, No 1, pp 1147-1157, 2017.

상세보기
R. Kah, H. Kappus. "Toxicology of the synthetic antioxidants BHA and BHT in comparison with the natural antioxidant vitamin E.", Z Lebensm Unters Forsch, Vol. 196, No. 4, pp. 329-338, 1993.

상세보기
VK. Gupta, SK. Sharma. "Plants as natural antioxidants." Natural Product Radiance, Vol. 5, No. 4, pp. 326-334, 2006.
HY. Hana, S. Shanb, X, Zhanga, "Down-regulation of prostate specific antigen in LNCaP cells by flavonoids from the pollen of Brassica napus L.", Phytomedicine, Vol. 14, pp. 338-343, 2007.

상세보기
I. Zila, A. Brozmanova, "Effects of Hypovolenia on Hypercapnic Ventilatory Response in Experimental Hyperthermia." J. Physiol. Pharmacol, Vol. 58, pp. 781-790, 2007.

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증