[논문]Defatting and Sonication Enhances Protein Extraction from Edible Insects

Choi, Byoung Deug; Wong, Nathan A.K.; Auh, Joong-Hyuck

doi:10.5851/kosfa.2017.37.6.955

Defatting and Sonication Enhances Protein Extraction from Edible Insects 원문보기

Korean journal for food science of animal resources = 한국축산식품학회지, v.37 no.6, 2017년, pp.955 - 961

Choi, Byoung Deug (Department of Food Science and Technology, Chung-Ang University) , Wong, Nathan A.K. (Department of Food Science and Technology, Chung-Ang University) , Auh, Joong-Hyuck (Department of Food Science and Technology, Chung-Ang University)

Abstract ▼ AI-Helper

Edible insects are attracting growing interest as a sustainable source of protein for addition to processed meat and dairy products. The current study investigated the optimal method for protein extraction from mealworm larvae (Tenebrio molitor), cricket adults (Gryllus bimaculatus), and silkworm pupae (Bombyx mori), for use in further applications. After defatting with n-hexane for up to 48 h, sonication was applied for 1-20 min and the protein yield was measured. All samples showed a total residual fat percentage below 1.36%, and a 35% to 94% improvement in protein yield (%). In conclusion, defatting with n-hexane combined with sonication improves the protein yield from insect samples.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

The amino acid composition of the defatted insect samples was analyzed using an Agilent 1100 HPLC with an Eclipse AAA column (4.5 × 150 mm, 5 µm) from Agilent Technologies (USA).

대상 데이터

A Sonics® Vibra-Cell™ VCX 750 ultrasonic unit (Sonics & Materials Inc., USA) was used, with a maximum power output of 2.5 kW.

데이터처리

Significance of differences among means was determined using analysis of variance (ANOVA) and Duncan’s multiple-range test, with a significance threshold of 5%.

이론/모형

Samples were sieved through a stainless-steel filter (pore size of 1 mm) and filtrates were collected and freeze-dried for further experiments. To determine protein amount and calculate protein yield, the samples were analyzed by the Dumas method using an NDA 701 Dumas Nitrogen Analyzer (Velp Scientifica, Italy). Protein yield (%) and total protein content of each insect sample were calculated based on protein quantity measured and reported as %N using the standard conversion factor of 6.
Samples were emptied onto aluminum foil and left to dry overnight under a fume hood. Total lipid content was determined using the Soxhlet extraction method(AOAC, 2000).

성능/효과

Conventional hexane defatting was carried out for 24- 48 h on mealworm, cricket, and silkworm samples. Our results showed average residual crude fat values of 0.75% for mealworm, 1.17% for cricket, and 0.47% for silkworm pupae. Our proximate composition analysis data for crude fat prior to defatting (33.
0% for all samples. The lowest average fat values, achieved after 48 h of defatting for mealworm, cricket, and silkworm samples, were 0.69%, 1.07% and 0.34%, respectively. No significant differences were observed after 36 h of defatting.
The protein content detected on a dry matter basis after defatting of each insect ranged from 62% to 74%, with the cricket samples displaying the highest values (results not shown). Research on proximate nutritional content showed similar values for all insects (Ghosh et al.
supercritical CO₂ (SCO₂). Total defatting percentages(%) were 96.56% for n-hexane and 92.57% for SCO₂ under conditions of 325 bars, 55℃, and 75 min. Although the SCO₂ method produced a more rapid result, the energy output also needs to be considered, and the two methods yielded products with similar physio-chemical properties and fat composition (Purschke et al.

참고문헌 (28)

AOAC Official methods of analysis of AOAC 17th Ed Association of Official Analytical Chemists Washington DC, USA 2000
Arzeni C. Perez O. E. Pilosof A. M. R. Functionality of egg white proteins as affected by high intensity ultrasound Food Hydrocolloid 2012 29 308 316 10.1016/j.foodhyd.2012.03.009

상세보기
Chandrapala J. Zisu B. Palmer M. Kentish S. Ashokkumar M. Effects of ultrasound on the thermal and structural characteristics of proteins in reconstituted whey protein concentrate Ultrason. Sonochem 2011 18 951 957 10.1016/j.ultsonch.2010.12.016 21262585

상세보기
Finke M. D. Complete nutrient composition of commercially raised invertebrates used as food for insectivores Zoo Biol. 2002 21 269 285 10.1002/zoo.10031

상세보기
Fishman S. M. Caulfield L. E. de Onis M. Blossner M. Hyder A. A. Mullany L. Black R. E. Ezzati M. A. D. Lopez Rodgers A. Murra C. J. L. Childhood and maternal undernutrition. In: Comparative quantification of health risks World Health Organization Switzerland 2002 39 161
Ghosh S. Lee S.-M. Jung C. Meyer-Rochow V. B. Nutritional composition of five commercial edible insects in South Korea J. Asia-Pac. Entomol. 2017 20 686 694 10.1016/j.aspen.2017.04.003

상세보기
Hall F. G. Jones O. G. O'Haire M. E. Liceaga A. M. Functional properties of tropical banded cricket (Gryllodes sigillatus) protein hydrolysates Food Chem. 2017 224 414 422 10.1016/j.foodchem.2016.11.138 28159288

상세보기
Jambrak A. R. Lelas V. Mason T. J. Kre？i G. Badanjak M. Physical properties of ultrasound treated soy proteins J. Food Eng. 2009 93 386 393 10.1016/j.jfoodeng.2009.02.001

상세보기
Karki B. Lamsal B. P. Jung S. van Leeuwen J. Pometto A. L. Grewell D. Khanal S. K. Enhancing protein and sugar release from defatted soy flakes using ultrasound technology J. Food Eng. 2010 96 270 278 10.1016/j.jfoodeng.2009.07.023

상세보기
Kim H.-W. Setyabrata D. Lee Y. J. Jones O. G. Kim Y. H. B. Pre-treated mealworm larvae and silkworm pupae as a novel protein ingredient in emulsion sausages Innov. Food Sci. Emerg. 2016 38 116 123 10.1016/j.ifset.2016.09.023

상세보기
L'Hocine L. Boye J. I. Yves A. Composition and functional properties of soy protein isolates prepared using alternative defatting and extraction procedures J. Food Sci. 2006 71 C137 C145 10.1111/j.1365-2621.2006.tb15609.x

상세보기
Lee H. Yildiz G. dos Santos L. C. Jiang S. Andrade J. E. Engeseth N. J. Feng H. Soy protein nano-aggregates with improved functional properties prepared by sequential pH treatment and ultrasonication Food Hydrocolloid 2016 55 200 209 10.1016/j.foodhyd.2015.11.022

상세보기
Longvah T. Mangthya K. Ramulu P. Nutrient composition and protein quality evaluation of eri silkworm (Samia ricinii) prepupae and pupae Food Chem. 2011 128 400 403 10.1016/j.foodchem.2011.03.041 25212147

상세보기
Lou Z. Wang H. Zhang M. Wang Z. Improved extraction of oil from chickpea under ultrasound in a dynamic system J. Food Eng. 2010 98 13 18 10.1016/j.jfoodeng.2009.11.015

상세보기
Melo V. Garcia M. Sandoval H. Jimenez H. D. Calvo C. Quality proteins from edible indigenous insect food of Latin America and Asia Emir. J. Food Agr. 2011 23 283 289
Omotoso O. T. Nutritional quality, functional properties and anti-nutrient compositions of the larva of Cirina forda (Westwood) (Lepidoptera: Saturniidae) J. Zhejiang Univ. Sci. B 2006 7 51 55 10.1631/jzus.2006.B0051 16365926

상세보기
Preece K. E. Hooshyar N. Krijgsman A. J. Fryer P. J. Zuidam N. J. Pilot-scale ultrasound-assisted extraction of protein from soybean processing materials shows it is not recommended for industrial usage J. Food Eng. 2017 206 1 12 10.1016/j.jfoodeng.2017.02.002

상세보기
Purschke B. Stegmann T. Schreiner M. Jager H. Pilot-scale supercritical CO 2 extraction of edible insect oil from Tenebrio molitor L. larvae - Influence of extraction conditions on kinetics, defatting performance and compositional propertie Eur. J. Lipid Sci. Tech. 2017 119 1600134 10.1002/ejlt.201600134

상세보기
Ricochon G. Muniglia L. Influence of enzymes on the oil extraction processes in aqueous media OCL 2010 17 356 359 10.1051/ocl.2010.0337

상세보기
Rumpold B. A. Schluter O. K. Potential and challenges of insects as an innovative source for food and feed production Innov. Food Sci. Emerg. 2013 17 1 11 10.1016/j.ifset.2012.11.005

상세보기
Tzompa-Sosa D. A. Yi L. van Valenberg H. J. F. van Boekel M. A. J. S. Lakemond C. M. M. Insect lipid profile: Aqueous versus organic solvent-based extraction methods Food Res. Int. 2014 62 1087 1094 10.1016/j.foodres.2014.05.052

상세보기
van Huis A. Itterbeeck J. V. Klunder H. Mertens E. Halloran A. Muir G. Vantomme P. Edible insects: Future prospects for food and feed security Food and Agriculture Organization of the United Nations 2013
Vilkhu K. Mawson R. Simons L. Bates D. Applications and opportunities for ultrasound assisted extraction in the food industry - A review Innov. Food Sci. Emerg. 2008 9 161 169 10.1016/j.ifset.2007.04.014

상세보기
Wang W. Wang N. Liu C. Jin J. Effect of silkworm pupae peptide on the fermentation and quality of yogurt J. Food Process. Pres. 2017 41 e12893 10.1111/jfpp.12893

상세보기
Yi L. Lakemond C. M. Sagis L. M. Eisner-Schadler V. van Huis A. van Boekel M. A. Extraction and characterisation of protein fractions from five insect species Food Chem. 2013 141 3341 3348 10.1016/j.foodchem.2013.05.115 23993491

상세보기
Yi L. Van Boekel M. A. J. S. Boeren S. Lakemond C. M. M. Protein identification and in vitro digestion of fractions from Tenebrio molitor Eur. Food Res. Technol. 2016 242 1285 1297 10.1007/s00217-015-2632-6

상세보기
Zhao X. Vazquez-Gutierrez J. L. Johansson D. P. Landberg R. Langton M. Yellow mealworm protein for food purposes - Extraction and functional properties PLoS One 2016 11 e0147791 10.1371/journal.pone.0147791 26840533

상세보기
Zieliska E. Baraniak B. Kara M. Rybczyska K. Jakubczyk A. Selected species of edible insects as a source of nutrient composition Food Res. Int. 2015 77 460 466 10.1016/j.foodres.2015.09.008

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증