[논문]ROS Scavenging and Anti-Wrinkle Effects of Clitocybin A Isolated from the Mycelium of the Mushroom Clitocybe aurantiaca

Lee, Joo-Eun; Lee, Ik-Soo; Kim, Kwan-Chul; Yoo, Ick-Dong; Yang, Han-Mo

doi:10.4014/jmb.1702.02050

ROS Scavenging and Anti-Wrinkle Effects of Clitocybin A Isolated from the Mycelium of the Mushroom Clitocybe aurantiaca 원문보기

Journal of microbiology and biotechnology, v.27 no.5, 2017년, pp.933 - 938

Lee, Joo-Eun (National Research Laboratory for Cardiovascular Stem Cell, Seoul National University College of Medicine and Department of Internal Medicine, Seoul National University Hospital) , Lee, Ik-Soo (KM Convergence Research Division, Korea Institute of Oriental Medicine) , Kim, Kwan-Chul (Innoskin Co., Ltd. BVC, Korea Research Institute of Bioscience and Biotechnology) , Yoo, Ick-Dong (Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology) , Yang, Han-Mo (National Research Laboratory for Cardiovascular Stem Cell, Seoul National University College of Medicine and Department of Internal Medicine, Seoul National University Hospital)

Abstract ▼ AI-Helper

Clitocybin A, an isoindolinone from Clitocybe aurantiaca, was investigated to assess its anti-wrinkle properties, through reactive oxygen species (ROS)-scavenging and elastase inhibitory activities, procollagen synthesis, and matrix metalloproteinase-1 (MMP-1) expression, in human primary dermal fibroblast-neonatal (HDF-N) cells. Clitocybin A exhibited no significant cytotoxicity up to 10 ppm in HDF-N cells, with cell viability and cell proliferation activity greater than 94.6% and 91.9%, respectively. Strong and concentration-dependent ROS radical scavenging activities of clitocybin A were observed following irradiation with UVB at $30mJ/cm^2$. Furthermore, clitocybin A treatment of cells at 0.1, 1, and 10 ppm exhibited decreased elastase activity, in a concentration-dependent manner, by 1.97%, 6.6%, and 8.31%, respectively, versus the control group. The effects of clitocybin A on procollagen synthesis and MMP-1 expression were investigated. Clitocybin A treatment of cells at 1, 5, and 10 ppm increased procollagen synthesis, by 67.9%, 74.4%, and 112.9%, respectively, versus the control group. At these concentrations, MMP-1 expression decreased significantly following UV irradiation. Together, these findings suggest that clitocybin A may be an effective ingredient for use in anti-wrinkle cosmetic products.

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가설 설정

(B) ROS-scavenging activity was examined under a fluorescence microscope. a: UVB non-irradiated control. b: UVB-irradiated control (30 mJ/cm²).
a: UVB non-irradiated control. b: UVB-irradiated control (30 mJ/cm²). c: Cells exposed to UVB at 30 mJ/cm² and treated with 2 mM of vitamin C.

제안 방법

The ROS-scavenging activity was determined to evaluate the protective effects of clitocybin A against oxidative damage (Fig. 4A). Cells irradiated with UVB at 30 mJ/cm² had a significantly increased level of ROS compared with non-irradiated cells.
This study was conducted to examine the potential of clitocybin A, isolated from C. aurantiaca, as an anti-wrinkle cosmetic ingredient. The anti-wrinkle effects of clitocybin A were evaluated with regard to ROS scavenging and elastase inhibitory activities, procollagen synthesis, and MMP-1 expression in human primary dermal fibroblastneonatal (HDF-N) cells.

데이터처리

For statistical analyses, Student’s t-test was used.

이론/모형

Effects of clitocybin A on HDF-N cell proliferation. Cell proliferation was assessed using the WST-1 assay. HDF-N cells were cultured in serum-free medium for 12 h and treated with clitocybin A at 1, 5, and 10 ppm for 48 h.
Cytotoxicity of clitocybin A against HDF-N cells. Cell viability was determined using the WST-1 assay. HDF-N cells were cultured in serum-free medium for 12 h and treated with clitocybin A at 0, 1, 5, 10, 50, and 100 ppm for 24 h.

성능/효과

Additionally, the antiwrinkle capacity of clitocybin A, as determined from ROSscavenging and elastase inhibitory activities, procollagen synthesis, and MMP-1 expression in HDF-N cells, was statistically significant and concentration-dependent. In conclusion, clitocybin A may be an effective ingredient in functional cosmetics to prevent or alleviate skin wrinkles induced by ultraviolet rays, given its ROS radicalscavenging capacity, effects on procollagen synthesis, and the inhibitory effects on elastase and MMP-1 expression levels, which directly affect the elasticity of skin tissues.

참고문헌 (25)

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ROS Scavenging and Anti-Wrinkle Effects of Clitocybin A Isolated from the Mycelium of the Mushroom Clitocybe aurantiaca 원문보기

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ROS Scavenging and Anti-Wrinkle Effects of Clitocybin A Isolated from the Mycelium of the Mushroom Clitocybe aurantiaca 원문보기

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