포제한약재의 최종당화산물 생성 억제 활성 및 항산화 효과 Inhibitory Activity of Advanced Glycation Endproducts (AGEs) Formation and Antioxidant Activity of Processed Korean Medicines원문보기
Objectives : Advanced glycation end product (AGEs) is combine formation of glucose and protein. AGEs and reactive oxygen species are potential therapeutic targets for the various disease such as diabetic complications, renal injury, skin damage. The aim of this study was investigated the AGEs inhibi...
Objectives : Advanced glycation end product (AGEs) is combine formation of glucose and protein. AGEs and reactive oxygen species are potential therapeutic targets for the various disease such as diabetic complications, renal injury, skin damage. The aim of this study was investigated the AGEs inhibitory activity and antioxidant activity of water extracts from 40 Korean medicines and 5 heating-processed Korean medicines. Methods: AGEs formation inhibitory activities of Korean medicines measured using bovine serum albumin (BSA), glucose, and fructose. Then, five effective Korean medicines were selected and heated with 30% ethanol. The AGEs inhibitory activities of heated Korean medicine were measured compared with not-heated Korean medicines. The antioxidant activities were evaluated through radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals. Furthermore, we examined total phenol and flavonoids contents. Results: Scutellariae Radix, Corni Fructus, Persimmon Fruit, Paeoniae Radix, Mori Folium respectively reduced AGEs production. Morever, heating-processed Scutellariae Radix has AGEs inhibitory activities better than not-processed Scutellariae Radix. Heating- processed Scutellariae Radix scavenged DPPH and ABTS effectively and $IC_{50}$ of DPPH and ABTS radical scavenging activity of Heat processed Scutellariae Radix were $15.47{\pm}0.26{\mu}g/m{\ell}$ and $12.07{\pm}1.23{\mu}g/m{\ell}$. It caused heat processing methods of Scutellariae Radix up regulated total phenol and flavonoids contents ($26.68{\pm}0.01$ to $46.15{\pm}0.10$, $20.30{\pm}0.38$ to $64.20{\pm}0.52$). Conclusion: It has AGEs inhibitory activities that 20 kind of medicinal plants of 40 medicinal plants. Especially, heat processed Scutellariae Radix has excellent AGEs inhibitory activities and antioxidant effect.
Objectives : Advanced glycation end product (AGEs) is combine formation of glucose and protein. AGEs and reactive oxygen species are potential therapeutic targets for the various disease such as diabetic complications, renal injury, skin damage. The aim of this study was investigated the AGEs inhibitory activity and antioxidant activity of water extracts from 40 Korean medicines and 5 heating-processed Korean medicines. Methods: AGEs formation inhibitory activities of Korean medicines measured using bovine serum albumin (BSA), glucose, and fructose. Then, five effective Korean medicines were selected and heated with 30% ethanol. The AGEs inhibitory activities of heated Korean medicine were measured compared with not-heated Korean medicines. The antioxidant activities were evaluated through radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals. Furthermore, we examined total phenol and flavonoids contents. Results: Scutellariae Radix, Corni Fructus, Persimmon Fruit, Paeoniae Radix, Mori Folium respectively reduced AGEs production. Morever, heating-processed Scutellariae Radix has AGEs inhibitory activities better than not-processed Scutellariae Radix. Heating- processed Scutellariae Radix scavenged DPPH and ABTS effectively and $IC_{50}$ of DPPH and ABTS radical scavenging activity of Heat processed Scutellariae Radix were $15.47{\pm}0.26{\mu}g/m{\ell}$ and $12.07{\pm}1.23{\mu}g/m{\ell}$. It caused heat processing methods of Scutellariae Radix up regulated total phenol and flavonoids contents ($26.68{\pm}0.01$ to $46.15{\pm}0.10$, $20.30{\pm}0.38$ to $64.20{\pm}0.52$). Conclusion: It has AGEs inhibitory activities that 20 kind of medicinal plants of 40 medicinal plants. Especially, heat processed Scutellariae Radix has excellent AGEs inhibitory activities and antioxidant effect.
따라서 본 연구는 국내외의 한약재 40종을 대상으로 glycation 억제효능 및 DPPH, ABTS 라디칼 소거능 및 총 페놀, 플라보노이드 함량을 실험하여 항산화 효능을 나타내는 소재를 검토하였다.
제안 방법
본 연구에서는 국내외 한약재 및 포제한 한약재를 열수 추출하여 최종당화산물 생성 억제활성 및 항산화 활성을 평가하였다. BSA에 glucose, fructose 및 1 ㎎/㎖의 시료를 가한 후 37℃에서 일주일간 최종당화산물을 유발하여 억제활성을 평가한 결과 黃芩, 山茱萸, 桑葉, 枾果, 白芍藥에서 뛰어난 최종당화산물 억제활성을 나타내었고 따라서 추후 연구에서는 5종의 시료를 대상으로 진행하였다.
대상 데이터
본 실험에 사용된 2.2-Diphenyl-1-picrylhydrazyl (DPPH), 7 mM 2,2'-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid) (ABTS), potassium phosphate monobasic, potassium phosphate dibasic, gallic acid, Folin-Ciocalteu's phenol reagent, diethylene glycol, sodium hydroxide, naringin, bovine serum albumin, glucose, fructose은 Sigma Aldrich (St Luis, MO, USA)에서 구입하여 사용하였다.
본 실험에서 사용한 한약재 중 枾果은 경상북도 상주시에서 채취하여 사용하였고, 枾果을 제외한 39종의 한약재는 옹기한약국 (Daegu, Korea)에서 구입하여 사용하였다. 최종당화산물 생성 억제활성 실험을 통하여 선별한 5종의 시료는 roasting machine (Genesis Co.
데이터처리
데이터는 평균±표준편차로 표현하였으며, SPSS (Version 22.0, IBM, Armonk, NY, USA)을 사용하여 one-way analysis of variance (ANOVA) test를 실시한 후 Student's t-test방법에 의하여 각 구간의 유의성 차이를 검증하였다(*p <0.05, **p <0.01, ***p <0.001).
성능/효과
1. 국내외의 한약재 40종 중 20종의 한약재는 최종당화산물 억제 활성을 나타내었고 그 중, 黃芩, 山茱萸, 枾果,白芍藥, 桑葉은 뛰어난 최종당화산물 억제 활성을 나타내었다.
2. 黃芩, 山茱萸, 枾果, 白芍藥, 桑葉을 酒炒하여 최종당화산물억제활성을 살펴본 결과 黃芩, 枾果, 白芍藥은 최종당화산물 억제 활성이 증가하였다.
3. 黃芩, 山茱萸, 枾果, 白芍藥, 桑葉 및 이를 酒炒하여DPPH 라디칼 활성 및 ABTS 라디칼 활성을 분석한 결과酒炒한 黃芩이 우수한 항산화 활성을 나타냄을 확인하였다.
후속연구
위와 같은 결과를 종합하여 보았을 때 黃芩 및 酒炒한 黃芩은 우수한 최종당화산물 억제활성과 항산화 활성을 가지고 있다고 사료되며 이와 관련하여 추후 연구가 진행된다면 기능성 식품 소재로서의 가치가 충분하다고 판단된다.
질의응답
핵심어
질문
논문에서 추출한 답변
DPPH free radical 소거법은 어떤 실험법인가?
항산화 활성을 측정하는 방법으로 Blosis에 의한 DPPH free radical 소거법을 사용하였다6). DPPH 라디칼 소거 활성은 시료 중에 포함된 항산화 물질의 양을 측정하는데 사용되는 대표적인 실험법이다. 일정농도의 시료 100 ㎕과 60 µM DPPH 용액을 100 ㎕넣고 혼합한 후, 실온에서 30분간 반응시켰다.
최종당화산물은 무엇인가?
최종당화산물 (Advanced glycation endproducts, AGEs)이란 혈중의 당이 높아졌을 때 환원당인 포도당이 단백질의 free amino group과 반응하여 단백질에 비효소적 당화 반응을 일으키고 단백질과 포도당사이에 공유결합을 이루게 된 형태를 말한다1). 당화 반응에 의한 최종당화산물의 생성은 당뇨성 동맥경화, 말초신경마비, 망막질환, 신장질환, 피부질환 등을 유발하는 것으로 알려져 있다.
당화반응에 의한 최종당화산물의 생성은 무엇을 유발하는가?
최종당화산물 (Advanced glycation endproducts, AGEs)이란 혈중의 당이 높아졌을 때 환원당인 포도당이 단백질의 free amino group과 반응하여 단백질에 비효소적 당화 반응을 일으키고 단백질과 포도당사이에 공유결합을 이루게 된 형태를 말한다1). 당화 반응에 의한 최종당화산물의 생성은 당뇨성 동맥경화, 말초신경마비, 망막질환, 신장질환, 피부질환 등을 유발하는 것으로 알려져 있다. 최종당화산물은 세포의 수용체를 거쳐 신호전달체계를 활성화하여 활성산소종 (reactive oxygen species, ROS)를 유도하며 전구물질을 분해하는 효소인 glyoxalase의 활성을 감소시켜 세포 내 스트레스를 가속화한다2).
참고문헌 (20)
Park SJ, Chung H. Outer blood-retinal barrier alteration induced by intraocular advanced glycation endproduct. J. Korean Ophthalmol. Soc. 2001 ; 42(2) : 151-5.
Kwon HO, Lee MH, Kim YJ, Kim E, Kim OK. Beneficial effects of Acanthopanax seticosus extract in type II diabetes animal model via down-regulation of advanced glycated hemoglobin and glycosylation end products. J. Korean Soc Food Sci Nutr. 2016 ; 45(7) : 929-37.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine. 1999 ; 26(9) : 1231-37.
Folin O, Denis W. On phosphotungasticphosphomolybdic compounds as color reagent. Journal of biological chemisty. 1912 ; 12(2) : 239-43.
Lister CE, Lancaster JE, Sutton KH, Walker JR. Developmental changes in the concentration and composition of flavonoids in skin of a red and a green apple cultivar. Journal of the science of food and agriculture. 1994 ; 64(2) : 155-61.
Maillard-Lefebvre H, Boulanger E, Daroux M, Gaxatte C, Hudson BI, Lambert M. Soluble receptor for advanced glycation end products: a new biomarker in diagnosis and prognosis of chronic inflammatory diseases. Rheumatology (Oxford) 2009 ; 48(10) : 1190-1196.
Kim BH, Son SM. Mechanism of Developing Diabetic Vascular Complication by Oxidative Stress. Endocrinology and Metabolism. 2006 ; 21(6) : 448-59.
Lee HS, Yoon JA. Inhibitory activity of advanced glycation endproducts (AGE) formation of edible plant for development of anti-wrinkle ingredients. J. Korean Soc Food Sci Nutr. 2010 ; 39(2) : 186-92.
Seo CS, Kim JH, Shin HK, Kim BS. Quantitative Analysis of (+)-Catechin, Paeoniflorin, and Paeonol in Moutan Radicis Cortex and Its Processed Products. Kor. J. Pharmacogn. 2016 ; 47(3) : 237-45
Kwon SR, Kim HG, Ham IH, Lee JJ, Lee JH, Hong SP, Kim DH, Choi HY. Studies on the changes of oligosaccharide contents in rehmanniae radix preparata according to various processing methods. Kor. J. Herbology. 2007 ; 22(4) : 261-70.
Nakgawa T, Yokozawa T, Kim YA, Kang KS. Tanaka T. Activity of wen-pi-tang, and purified constituents of rhei rhizome and glycyrrhizae radix against glucose-mediated protein damage. Am. J. Chin. Med. 2005 ; 33(5) : 817-29.
Kim JM, Jang DS, Lee YM, Yoo JL, Kim YS, Kim JH, Kim JS. Aldose-reductase- and proteinglycation-inhibitory principles from the whole plant of Duchesnea chrysantha. Chem. Biodivers. 2008 ; 5(2) : 352-6.
Shin SH, Shin YO, Lee JY, Lee AR, Kim MY, Park CH, Seo BI, Roh SS. Ethanol-Heated Processed Scutellariae Radix Improve Inflammatory Response through an Inhibitory Effect against Oxidative Stress in Mice with the Lipopolysaccharide-induced Intestine Injury of Mice. Kor. J. Herbology. 2015 ; 30(4) : 81-8.
Kim NY. Effect of Antioxidation and Inhibition of Melanogenesis from Scutellaria baicalensis Extract. Kor. J. Aesther. Cosmetol. 2014 ; 12(1) : 41-7.
Ham IH, Maeng WM, Yang GS, Kim DH, Kim DH, Cho JH, Choi HY. Study on the Variation of Components from Scutellariae Radix by Processing and Storage Condition. Kor. J. Herbology. 2007 ; 22(2) : 189-99.
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