한국 서해안에 서식하는 주황해변해면에서 분리된 해양세균 Microbulbifer sp.으로부터 생리활성물질 비올라세인의 규명 Identification of a Bioactive Compound, Violacein, from Microbulbifer sp. Isolated from a Marine Sponge Hymeniacidon sinapium on the West Coast of Korea원문보기
오늘날 해양생물로부터 얻어진 미생물유래의 이차대사물질은 구조적, 생물학적으로 새로운 화합물의 주요한 자원이다. 그 중에서 해면동물과 미생물 관계는 생리활성 물질을 탐색하는데 가장 흥미있는 자원 중 하나로서 주목받아 왔다. 본 연구에서는 서해안 조간대에서 채집된 주황해변해면(Hymeniacidon sinapium)으로부터 분리된 세균 균주(Microbulbifer sp., 127CP-12)를 검토하였다. 배양된 세균은 자주색 색소를 생산하였으며, 색소생산의 최적 배양조건을 조사하였다. 최대 색소생산을 위한 미생물 배양조건은 $25^{\circ}C$, pH 6.0, 3% NaCl임을 알 수 있었다. 추출용매는 에탄올과 메탄올에 비해 아세톤이 더 적절한 것으로 나타났다. 추출된 색소의 주요성분은 HLPC, NMR, MS, 그리고 UV 스펙트럼의 구조 분석을 통해 유용한 생리활성물질인 비올라세인으로 밝혀졌다. 본 연구는 해양미생물이 관여한 대사물질로부터 생리활성물질을 조사하는 연구기법을 서술함과 동시에 오늘날 변화하는 해양환경에서 해면동물과 미생물 관계의 생태학적 의의를 제시하고 있다.
오늘날 해양생물로부터 얻어진 미생물유래의 이차대사물질은 구조적, 생물학적으로 새로운 화합물의 주요한 자원이다. 그 중에서 해면동물과 미생물 관계는 생리활성 물질을 탐색하는데 가장 흥미있는 자원 중 하나로서 주목받아 왔다. 본 연구에서는 서해안 조간대에서 채집된 주황해변해면(Hymeniacidon sinapium)으로부터 분리된 세균 균주(Microbulbifer sp., 127CP-12)를 검토하였다. 배양된 세균은 자주색 색소를 생산하였으며, 색소생산의 최적 배양조건을 조사하였다. 최대 색소생산을 위한 미생물 배양조건은 $25^{\circ}C$, pH 6.0, 3% NaCl임을 알 수 있었다. 추출용매는 에탄올과 메탄올에 비해 아세톤이 더 적절한 것으로 나타났다. 추출된 색소의 주요성분은 HLPC, NMR, MS, 그리고 UV 스펙트럼의 구조 분석을 통해 유용한 생리활성물질인 비올라세인으로 밝혀졌다. 본 연구는 해양미생물이 관여한 대사물질로부터 생리활성물질을 조사하는 연구기법을 서술함과 동시에 오늘날 변화하는 해양환경에서 해면동물과 미생물 관계의 생태학적 의의를 제시하고 있다.
Microbial secondary metabolites of marine organisms are regarded as major sources of structurally and biologically novel compounds with numerous potential uses. Sponge-microbe associations are among the most interesting sources for exploring bioactive compounds. In this study, the bacterial strain M...
Microbial secondary metabolites of marine organisms are regarded as major sources of structurally and biologically novel compounds with numerous potential uses. Sponge-microbe associations are among the most interesting sources for exploring bioactive compounds. In this study, the bacterial strain Microbulbifer sp. (127CP7-12) was isolated from the Asian marine sponge Hymeniacidon sinapium collected at an intertidal zone on the west coast of Korea. Cultured bacteria produced a violet pigment, and optimal culture conditions for violet pigment production were investigated. Maximum production of the violet pigment from the strain culture was observed under the conditions of $25^{\circ}C$, pH 6.0, and 3% NaCl. Acetone provided better extraction of the pigment from fermented broth compared with ethanol and methanol. The proposed structure of the major component in the extracted crude pigment was determined via high-performance liquid chromatography, nuclear magnetic resonance, mass spectrometry, and UV spectra analyses, which showed that the metabolite was the promising bioactive compound violacein. This study describes the examination of marine bioactive materials from microbe-engaged metabolites and the ecological implications of the sponge-microbe association in a changing ocean.
Microbial secondary metabolites of marine organisms are regarded as major sources of structurally and biologically novel compounds with numerous potential uses. Sponge-microbe associations are among the most interesting sources for exploring bioactive compounds. In this study, the bacterial strain Microbulbifer sp. (127CP7-12) was isolated from the Asian marine sponge Hymeniacidon sinapium collected at an intertidal zone on the west coast of Korea. Cultured bacteria produced a violet pigment, and optimal culture conditions for violet pigment production were investigated. Maximum production of the violet pigment from the strain culture was observed under the conditions of $25^{\circ}C$, pH 6.0, and 3% NaCl. Acetone provided better extraction of the pigment from fermented broth compared with ethanol and methanol. The proposed structure of the major component in the extracted crude pigment was determined via high-performance liquid chromatography, nuclear magnetic resonance, mass spectrometry, and UV spectra analyses, which showed that the metabolite was the promising bioactive compound violacein. This study describes the examination of marine bioactive materials from microbe-engaged metabolites and the ecological implications of the sponge-microbe association in a changing ocean.
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가설 설정
The specific production of violacein as a primary component of violet pigment, mg product per gram of cells, was not presented in this study and is beyond the scope to be discussed from the presented results; however, the production level can be inferred from the production of violet pigment as a proxy of violacein. In the study, pigment production was closely related with bacterial growth. The optimal incubation conditions were consistent with previous results using Microbulbifer species [24].
대상 데이터
The entire purification procedure was repeated three times and pigment was isolated as pure compound at the retention time of 29 min under the HPLC conditions. 1 H NMR spectra were recorded on a Bruker Avance 600 MHz spectrometer at the National Center for Inter-University Research Facilities (NCIRF) at Seoul National University.
The sponge, H. sinapium, was collected by hand from an intertidal rocky shore on the western coast of Korea. The organism was transported to the laboratory after washing with sterilized artificial seawater and used for isolation of bacteria within 12 h [21].
후속연구
[10], there are many factors affecting the violacein production level, and these should be considered carefully in comparing various published values. During the present study, the preliminary yield of violet pigment was 0.25 g/l (unpublished) and further studies need to be performed to suggest both the maximum concentration and the productivity of violacein from the suggested Microbulbifer strain.
참고문헌 (30)
Halpern BS, Walbridge S, Selkoe KA, Kappel CV, Micheli F, D'Agrosa C, et al. 2008. A global map of human impact on marine ecosystems. Science 319: 948-952.
Poloczanska ES, Brown CJ, Sydeman WJ, Kiessling W, Schoeman DS, Moore PJ, et al. 2013. Global imprint of climate change on marine life. Nature Climate Change 3: 919-925.
Moran XAG, Alonso-Saez L, Nogueira E, Ducklow HW, Gonzalez N, Lopez-Urrutia A, et al. 2015. Presented at the Proc. R. Soc. B.
Barton AD, Irwin AJ, Finkel ZV, Stock CA. 2016. Anthropogenic climate change drives shift and shuffle in North Atlantic phytoplankton communities. Proceedings of the National Academy of Sciences 113: 2964-2969.
Harvey AL, Edrada-Ebel R, Quinn RJ. 2015. The re-emergence of natural products for drug discovery in the genomics era. Nature Reviews. Drug Discovery 14: 111-129.
D'Orazio N, Gammone MA, Gemello E, De Girolamo M, Cusenza S, Riccioni G. 2012. Marine bioactives: pharmacological properties and potential applications against inflammatory diseases. Marine Drugs 10: 812-833.
Choi SY, Kim S, Lyuck S, Kim SB, Mitchell RJ. 2015. High-level production of violacein by the newly isolated Duganella violaceinigra str. NI28 and its impact on Staphylococcus aureus. Scientific Reports 5: 15598.
El-Shitany NA, Shaala LA, Abbas AT, Abdel-Dayem UA, Azhar EI, Ali SS, et al. 2015. Evaluation of the anti-inflammatory, antioxidant and immunomodulatory effects of the organic extract of the red sea marine sponge xestospongia testudinaria against carrageenan induced rat paw inflammation. PLoS One 10: e0138917.
Park MH, Sim CJ, Baek J, Min GS. 2007. Identification of genes suitable for DNA barcoding of morphologically indistinguishable Korean Halichondriidae sponges. Mol. Cells 23: 220-227.
Fuller T, Hughey J. 2013. Molecular investigation of the invasive sponge Hymeniacidon sinapium (de Laubenfels, 1930) in Elkhorn Slough, California. Aquatic Invasions 8: 59-66.
Jeong J-B, Park J-S. 2012. Seasonal differences of bacterial communities associated with the marine sponge, hymeniacidon sinapium. The Korean J. Microbiol. 48: 262-269.
Yang LH, Xiong H, Lee OO, Qi SH, Qian PY. 2007. Effect of agitation on violacein production in Pseudoalteromonas luteoviolacea isolated from a marine sponge. Lett. Appl. Microbiol. 44: 625-630.
Wakabayashi M, Sakatoku A, Noda F, Noda M, Tanaka D, Nakamura S. 2012. Isolation and characterization of Microbulbifer species 6532A degrading seaweed thalli to single cell detritus particles. Biodegradation 23: 93-105.
Rettori D, Duran N. 1998. Production, extraction and purificationof violacein: an antibiotic pigment producedby Chromobacterium violaceum. World J. Microbiol. Biotechnol. 14: 685-688.
Nakamura Y, Sawada T, Morita Y, Tamiya E. 2002. Isolation of a psychrotrophic bacterium from the organic residue of a water tank keeping rainbow trout and antibacterial effect of violet pigment produced from the strain. Biochem. Eng. J. 12: 79-86.
Yoon JH, Kim H, Kang KH, Oh TK, Park YH. 2003. Transfer of Pseudomonas elongata Humm 1946 to the genus Microbulbifer as Microbulbifer elongatus comb. nov. Int. J. Syst. Evol. Microbiol. 53: 1357-1361.
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