[국내논문]Supplemental effects of biofloc powder on growth performance, innate immunity, and disease resistance of Pacific white shrimp Litopenaeus vannamei원문보기
An 8-week feeding trial was conducted to study the effect of dietary supplementation of a biofloc powder on growth performance and non-specific immune response of Litopenaeus vannamei. Seven experimental diets were prepared with supplementation of graded levels of dried biofloc powder by 0, 0.5, 1.0...
An 8-week feeding trial was conducted to study the effect of dietary supplementation of a biofloc powder on growth performance and non-specific immune response of Litopenaeus vannamei. Seven experimental diets were prepared with supplementation of graded levels of dried biofloc powder by 0, 0.5, 1.0, 2.0, 4.0, 6.0, and 8.0% (designated as Con, BF0.5, BF1, BF2, BF4, BF6, and BF8, respectively). Triplicate groups of shrimp ($1.01{\pm}0.01g$) were hand-fed with one of the diets four times a day. At the end of the feeding trial, significantly (P<0.05) higher growth performance and feed utilization were obtained in BF4 groups compared to those fed the Con diet. The innate immunity of shrimp was improved by the dietary supplementation of biofloc. Dietary inclusion of biofloc at the level of 4.0% significantly increased disease resistance of shrimp against Vibrio harveyi. The results indicate that biofloc might be used as a dietary supplement for growth performance, innate immunity and disease resistance of Pacific white shrimp.
An 8-week feeding trial was conducted to study the effect of dietary supplementation of a biofloc powder on growth performance and non-specific immune response of Litopenaeus vannamei. Seven experimental diets were prepared with supplementation of graded levels of dried biofloc powder by 0, 0.5, 1.0, 2.0, 4.0, 6.0, and 8.0% (designated as Con, BF0.5, BF1, BF2, BF4, BF6, and BF8, respectively). Triplicate groups of shrimp ($1.01{\pm}0.01g$) were hand-fed with one of the diets four times a day. At the end of the feeding trial, significantly (P<0.05) higher growth performance and feed utilization were obtained in BF4 groups compared to those fed the Con diet. The innate immunity of shrimp was improved by the dietary supplementation of biofloc. Dietary inclusion of biofloc at the level of 4.0% significantly increased disease resistance of shrimp against Vibrio harveyi. The results indicate that biofloc might be used as a dietary supplement for growth performance, innate immunity and disease resistance of Pacific white shrimp.
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제안 방법
Each aquarium was supplied with filtered seawater and aeration to maintain enough dissolved oxygen. Triplicate groups of shrimp were hand-fed with one of the test diets four times a day at 08:00, 12.00, 16.00, and 18:00 h for 8 weeks. Daily feeding rates were slowly reduced from 10 to 8% of wet body weight during the 8-week feeding period.
CR and SH conducted the feeding trial, analysis, and drafted the manuscript. SJ manufactured the experimental diet.
SJ manufactured the experimental diet. KJ designed this study, led in analyzing data derived from this study, and revised the manuscript. All authors read and approved the final manuscript.
대상 데이터
The feeding trial was conducted in indoor shrimp culture facilities at the Marine Science Institute of the Jeju National University (Jeju, South Korea). Juvenile L.
데이터처리
All dietary treatments were assigned by a completely randomized design. Data were subjected to one-way analysis of variance (ANOVA) in SPSS version 12.0 (SPSS Inc., Chicago, IL, USA). When ANOVA identified differences among groups, the difference in means was made with LSD multiple range tests.
, Chicago, IL, USA). When ANOVA identified differences among groups, the difference in means was made with LSD multiple range tests. Statistical significance was determined at P ˂ 0.
이론/모형
Crude protein was measured by using an automatic Kjeltec Analyzer Unit 2300 (Foss Tecator, Höganäs, Sweden), and crude lipid was determined using the Soxhlet method with extraction in diethyl ether (Soxhlet Extraction System C-SH6, Korea).
, LTD, Japan). Diluted hemolymph protein content was measured using a microprotein determination method (C-690; Sigma). Oxidative radical production by hemocytes during respiratory burst was measured through the nitro blue tetrazolium (NBT) assay described by Dantzler et al.
참고문헌 (41)
Ajiboye OO, Yakubu AF, Adams TE. A perspective on the ingestion and nutritional effects of feed additives in farmed fish species. World J Fish & Marine Sci. 2012;4: 87-101.
Anand PSS, Kumar S, Panigrahi A, Ghoshal TK, Dayal JS, Biswas G, et al. Effects of C:N ratio and substrate integration on periphyton biomass, microbial dynamics and growth of Penaeus monodon juveniles. Aquaculture Int. 2013; 21:511-24.
Avnimelech Y. Biofloc technology-a practical guide book. 2nd ed. Baton Rouge: The World Aquaculture Society; 2012.
Bauer W, Prentice-Hernandez C, Tesser MB, Wasielesky W, Poersch LHS. Substitution of fishmeal with microbial floc meal and soy protein concentrate in diets for the pacific white shrimp Litopenaeus vannamei. Aquaculture. 2012;342-343:112-6.
Burford MA, Thompson PJ, McIntosh RP, Bauman RH, Pearson DC. The contribution of flocculated material to shrimp (Litopenaeus vannamei) nutrition in a high-intensity, zero-exchange system. Aquaculture. 2004; 232:525-37.
Castex M, Lemaire P, Wabete N, Chim L. Effect of dietary probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress status of shrimp Litopenaeus stylirostris. Aquaculture. 2009;294:306-13.
Castex M, Lemaire P, Wabete N, Chim L. Effect of probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress of Litopenaeus stylirostris under Vibrio nigripulchritudo challenge. Fish Shellfish Immunol. 2010;28:622-31.
Crab R, Lambert A, Defoirdt T, Bossier P, Verstraete W. The application of bioflocs technology to protect brine shrimp (Artemia franciscana) from pathogenic Vibrio harveyi. J Appl Microbiol. 2010;109:1643-9.
Dantzler AS, Burnett KG, Burnett LE. Effects of hypercapnic hypoxia and respiratory burst inhibition on the bactericidal activity of hemocytes from the penaeid shrimp Litopenaeus vannamei. Am Zool. 2001;41:1422-3.
De Schryver P, Crab R, Defoirdt T, Boon N, Verstraete W. The basics of bioflocs technoloty: the added value for aquaculture. Aquaculture. 2008;277:125-37.
Ekasari J, Azhar MH, Surawidjaja EH, Nuryati S, De Schryver P, Bossier P. Immune response and disease resistance of shrimp fed biofloc grown on different carbon sources. Fish Shellfish Immunol. 2014;41:332-9.
Hari RE, Livingstone DM, Siber R, Burkhardt-Holm PA, Guettinger H. Consequences of climatic change for water temperature and brown trout populations in Alpine rivers and streams. Glob Change Biol. 2006;12:10-26.
Haslun J, Correia E, Strychar K, Morris T, Samocha T. Characterization of bioflocs in a no water exchange super-intensive system for the production of food size pacific white shrimp Litopenaeus vannamei. Int J Aquacult. 2012; doi: 10.5376/ija.2012.02.0006
Hernandez-Lopez J, Gollas-Galvan T, Vargas-Albores F. Activation of the prophenoloxidase system of the brown shrimp (Penaeus californiensis Holmes). Comp biochem phys C. 1996;113:61-6.
Jang IK, Pang Z, Yu J, Kim SK, Seo HC, Cho YR. Selectively enhanced expression of prophenoloxidase activating enzyme 1 (PPAE1) at a bacteria clearance site in the white shrimp, Litopenaeus vannamei. BMC Immunol. 2011;12:70.
Johnson CN, Barnes S, Ogle J, Grimes DJ, Chang YJ, Peacock AD, Kline L. Microbial community analysis of water, foregut, and hindgut during growth of pacific white shrimp, Litopenaeus vannamei, in closed-system aquaculture. J World Aquac Soc. 2008;39:251-8.
Ju ZY, Forster IP, Conquest L, Dominy W. Enhanced growth effects on shrimp (Litopenaeus vannamei) from inclusion of whole shrimp floc or floc fractions to a formulated diet. Aquacult Nutr. 2008;14:533-43.
Kent M, Browd CL, Leffler JW. Consumption and digestion of suspended microbes by juvenile Pacific white shrimp Litopenaeus vannamei. Aquaculture. 2011;319:363-8.
Kiessling A, Askbrandt S. Nutritive value of two bacterial strains of single-cell protein for rainbow trout (Oncorhynchus mykiss). Aquaculture. 1993;109:119-30.
Kuhn DD, Boardman GD, Lawrence AL, Marsh L, Flick GJ. Microbial floc meal as a replacement ingredient for fish meal and soybean protein in shrimp feed. Aquaculture. 2009;296:51-7.
Kuhn DD, Lawrence AL, Boardman GD, Patnaik S, Marsh L, Flick GJ. Evaluation of two types of bioflocs derived from biological treatment of fish effluent as feed ingredients for Pacific white shrimp, Litopenaeus vannamei. Aquaculture. 2010;303:28-33.
Lee BJ, Kim SS, Song JW, Oh DH, Cha JH, Jeong JB, Heo MS, Kim KW, Lee KJ. Effects of dietary supplementation of citrus by-products fermented with a probiotic microbe on growth performance, innate immunity and disease resistance against Edwardsiella tarda in juvenile olive flounder, Paralichthys olivaceus (Temminck & Schlegel). J Fish Dis. 2013;36:617-28.
Li P, Gatlin DM. Dietary brewers yeast and the prebiotic $Grobiotic^{TM}$ AE influence growth performance, immune responses and resistance of hybrid striped bass (Morone chrysops $\times$ M. saxatilis) to Streptococcus iniae infection. Aquaculture. 2004;231:445-56.
Li J, Tan B, Mai K. Dietary probiotic Bacillus OJ and isomaltooligosaccharide influence the intestine microbial populations, immune responses and resistance to white spot syndrome virus in shrimp (Litopenaeus vannamei). Aquaculture. 2009;291:35-40.
Liu CH, Chen JC. Effect of ammonia on the immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus. Fish Shellfish Immunol. 2004;16:321-34.
Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Transl Res. 1967;70: 158-69.
Parrilla-Taylor DP, Zenteno-Savin T. Antioxidant enzyme activities in Pacific white shrimp (Litopenaeus vannamei) in response to environmental hypoxia and reoxygenation. Aquaculture. 2011;318:379-83.
Robertson L, Bray W, Leung-Truillo J, Lawrence A. Practical molt staging of Penaeus setiferus and Penaeus stylirostris. J World Aquac Soc. 1987;18:180-5.
Siwicki AK, Anderson DP, Rumsey GL. Dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis. Vet Immunol Immunop. 1994;41:125-39.
Wasielesky W, Atwood H, Stokes A, Browdy CL. Effect of natural production in a zero exchange suspended microbial floc based super-intensive culture system for white shrimp Litopenaeus vannamei. Aquaculture. 2006;258:396-403.
Wongsasak U, Chaijamrus S, Kumkhong S, Boonanuntanasarn S. Effects of dietary supplementation with ${\beta}$ -glucan and synbiotics on immune gene expression and immune parameters under ammonia stress in Pacific white shrimp. Aquaculture. 2015;436:179-87.
Xu WJ, Pan LQ, Zhao DH, Huang J. Preliminary investigation into the contribution of bioflocs on protein nutrition of Litopenaeus vannamei fed with different dietary protein levels in zero-water exchange culture tanks. Aquaculture. 2012;350-353:147-53.
Xu WJ, Pan LQ. Effects of bioflocs on growth performance, digestive enzyme activity and body composition of juvenile Litopenaeus vannamei in zero-water exchange tanks manipulating C/N ratio in feed. Aquaculture. 2012;356-357:147-52.
Xu WJ, Pan LQ. Enhancement of immune response and antioxidant status of Litopenaeus vannamei juvenile in biofloc-based culture tanks manipulating high C/N ratio of feed input. Aquaculture. 2013;412-413:117-24.
Zhao P, Huang J, Wang XH, Song XL, Yang CH, Zhang XG, et al. The application of bioflocs technology in high-intensive, zero exchange farming systems of Marsupenaeus japonicus. Aquaculture. 2012;354-355:97-106.
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