[논문]Comparative Analysis of Tissue and Cell Cycle on the Far Eastern Catfish, Silurus asotus between Diploid and Triploid

Gil, Hyun Woo; Lee, Tae Ho; Han, Ho Jae; Park, In-Seok

doi:10.12717/dr.2017.21.2.193

Comparative Analysis of Tissue and Cell Cycle on the Far Eastern Catfish, Silurus asotus between Diploid and Triploid 원문보기

Development & reproduction = 발생과 생식, v.21 no.2, 2017년, pp.193 - 204

Gil, Hyun Woo (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University) , Lee, Tae Ho (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University) , Han, Ho Jae (College of Veterinary Medicine and Research Institute of Veterinary Science, Seoul National University) , Park, In-Seok (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University)

Abstract ▼ AI-Helper

The influence of triploidization on histological characteristics of retina, trunk kidney, liver and midgut tissue, and cell cycle of tail fin and gill tissue in far eastern catfish, Silurus asotus were analyzed. In the infertile triploid fish, the nucleus and/or cell size of secondary proximal tubule cells of trunk kidney, hepatocyte and midgut epithelium are much larger than those of the corresponding cells in the diploid fish (P<0.05). However, triploid tissue showed fewer number of outer nuclear layer in retina and nuclei in secondary proximal tubule of trunk kidney than those for diploid tissue. The mean percentages of the $G_l-$, the S- and the $G_2+M-phase$ fractions were 92.5%, 3.2% and 4.3% in tail fin tissue of diploid, and 93.4%, 2.6% and 4.0% in those of triploid, respectively. There were no significant differences in the percentages of each cell cycle fraction between diploid and triploid. The mean percentages of each phase fractions were 75.1%, 11.1% and 13.8% in gill tissue of diploid and 85.2%, 8.9% and 5.9% in those of triploid, respectively. The differences of cell cycle between tail fin tissue and gill tissue were statistically significant in diploid and triploid (P<0.05). Also, the differences between diploid and triploid were statistically significant in tail fin tissue and gill tissue (P<0.05). Cyclin D1 and cyclin E expressions were not significantly difference between gill tissue and tail fin tissue, and protein expressions of induced triploid were higher than those of diploid. Results from this study suggest that some characteristics in the triploid exhibiting larger cell and nucleus size with fewer number of cell than diploid can be used as an indicator in the identification of triploidization and ploidy level in far eastern catfish.

주제어

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문제 정의

The aim of this study is to conduct a comparative histological analysis of retina, kidney, liver and midgut tissue by looking at cellular and nuclear size, and conduct a comparative analysis of cell cycle from tissues of diploid and triploid far eastern catfish.

제안 방법

Reduced gonadal growth in induced triploids may allow increased energy allocation to somatic growth, but any growth advantage may be offset by diminished levels of gonadal steroids, which have an anabolic effect (Benfey, 1999). This study has been able to verify certain factual information, which in conjunction with other characteristics, can be used as indicators in the identification of triploidization and ploidy level in far eastern catfish.

대상 데이터

). Ten specimens were used for histological observations from each group. Fish were euthanized with an overdose of lidocaine-HCl (300 ppm at 22℃, Park et al.

데이터처리

The differences among groups were analyzed using Student’s t-test from the SPSS statistics package (SPSS 9.0, SPSS Inc. Chicago, IL, USA).

이론/모형

Comments from anonymous reviewers greatly improved the quality of this manuscript. All experiments in this study complied with the current law of Korea (the law regarding experimental animals, No. 9932).
Sperm was obtained by incision of surgically removed testes that had also been given an IP injection of hCG at 500 IU kg/BW. Eggs were fertilized with sperm diluted in saline using the wet method (Kim et al., 2001; Seol et al., 2008). The eggs were left to fertilize for 5 mins after which they were rapidly rinsed to remove excess sperm and immediately subjected to cold-shock treatment (4℃) for 60 mins in order to prevent the onset of the second polar body.

성능/효과

In this study, mitosis of diploid in each tissue was more active than those of induced triploid far eastern catfish in each tissue, and mitosis of gill tissue in each ploidy was more active than those of tail fin tissue in each ploidy. Merely, diploid and induced triploid far eastern catfish using this experiment was 3 years after hatched, and the measured time was spawning season, when diploid had matured gonad but induced triploid had maintained sterility of a gonad.
If protein expressions of cyclin D1 and cyclin E are higher, then cell cycle is shorter. In this study, proteins expressions of both cyclin in diploid were higher than those in induced triploid, and cell cycle of diploid was shorter than that of induced triploid.
On the other hand, the mean percentages of the Gl-, the S- and the G2+M -phase fractions between diploid and triploid had shown significantly difference in gill tissue (P<0.05), and the mean percentages of each phase fractions were 75.1%, 11.1% and 13.8% in gill tissue of diploid and 85.2%, 8.9% and 5.9% in those of triploid, respectively (Table 4).
The mean percentages of the Gl-, the S- and the G2+M phase fractions were 92.5%, 3.2% and 4.3% in tail fin tissue of diploid, and 93.4%, 2.6% and 4.0% in those of triploid, respectively (Table 4).

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