Antibacterial compounds are widely used in the treatment of human and animal diseases. The overuse of antibiotics has led to a rapid rise in the prevalence of drug-resistant bacteria, making the development of new antibacterial compounds essential. This study focused on developing a fast and easy me...
Antibacterial compounds are widely used in the treatment of human and animal diseases. The overuse of antibiotics has led to a rapid rise in the prevalence of drug-resistant bacteria, making the development of new antibacterial compounds essential. This study focused on developing a fast and easy method for identifying marine bacteria that produce antibiotic compounds. Eight randomly selected marine target bacterial species (Agrococcus terreus, Bacillus algicola, Mesoflavibacter zeaxanthinifaciens, Pseudoalteromonas flavipulchra, P. peptidolytica, P. piscicida, P. rubra, and Zunongwangia atlantica) were tested for production of antibacterial compounds against four strains of test bacteria (B. cereus, B. subtilis, Halomonas smyrnensis, and Vibrio alginolyticus). Colony picking was used as the primary screening method. Clear zones were observed around colonies of P. flavipulchra, P. peptidolytica, P. piscicida, and P. rubra tested against B. cereus, B. subtilis, and H. smyrnensis. The efficiency of colony scraping and broth culture methods for antimicrobial compound extraction was also compared using a disk diffusion assay. P. peptidolytica, P. piscicida, and P. rubra showed antagonistic activity against H. smyrnensis, B. cereus, and B. subtilis, respectively, only in the colony scraping method. Our results show that colony picking and colony scraping are effective, quick, and easy methods of screening for antibacterial compound-producing bacteria.
Antibacterial compounds are widely used in the treatment of human and animal diseases. The overuse of antibiotics has led to a rapid rise in the prevalence of drug-resistant bacteria, making the development of new antibacterial compounds essential. This study focused on developing a fast and easy method for identifying marine bacteria that produce antibiotic compounds. Eight randomly selected marine target bacterial species (Agrococcus terreus, Bacillus algicola, Mesoflavibacter zeaxanthinifaciens, Pseudoalteromonas flavipulchra, P. peptidolytica, P. piscicida, P. rubra, and Zunongwangia atlantica) were tested for production of antibacterial compounds against four strains of test bacteria (B. cereus, B. subtilis, Halomonas smyrnensis, and Vibrio alginolyticus). Colony picking was used as the primary screening method. Clear zones were observed around colonies of P. flavipulchra, P. peptidolytica, P. piscicida, and P. rubra tested against B. cereus, B. subtilis, and H. smyrnensis. The efficiency of colony scraping and broth culture methods for antimicrobial compound extraction was also compared using a disk diffusion assay. P. peptidolytica, P. piscicida, and P. rubra showed antagonistic activity against H. smyrnensis, B. cereus, and B. subtilis, respectively, only in the colony scraping method. Our results show that colony picking and colony scraping are effective, quick, and easy methods of screening for antibacterial compound-producing bacteria.
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제안 방법
All target marine bacteria were randomly selected for the experiment. They belong to five genera (Agrococcus, Bacillus, Mesoflavibacter, Pseudoalteromonas, and Zunongwangia) and some of them have shown bactericidal effects in previous studies.
In conclusion, this study was conducted to find an efficient method to screen antibacterial compound-producing marine bacteria. The colony picking method provides a fast, efficient, and inexpensive antibacterial compound-producing bacteria screening process.
The rapid spread of multidrug resistance in bacteria is leading to reemerging and newly emerging infectious diseases, creating an urgent need for new classes of antibiotics[4]. In this study, we tested two preliminary screening methods for the rapid identification of marine bacteria that produce antibacterial compounds.
대상 데이터
All strains of marine bacteria (Table 1) used in this study were from stocks maintained at Jeju International Ocean Science Center for Research & Education (Korea Institute of Ocean Science & Technology, Korea).
In particular, the gram negativity of marine bacteria has led them to thrive in harsh environments, resulting in ranges of structurally diversified secondary metabolites [16]. Bacillus cereus, Bacillus subtilis, Halomonas smyrnensis, and Vibrio alginolyticus were used as test bacteria. B.
Strains used for this study were cultured on marine agar (Becton Dickinson Company, USA) and in marine broth (Becton Dickinson Company) at 30°C. Eight strains of test bacteria and four of target bacteria were used in this study.
이론/모형
45 µm disposable membrane filter unit. Both samples were used for the antagonistic activity test by disk diffusion assay.
성능/효과
3C). The antagonistic activity of all test bacteria on all target bacteria was higher in the colony scraping method than in the broth culture method, with the exception of P. rubra against H. smyrnensis (Fig. 4). P.
alginolyticus. The other four bacterial species (A. terreus, M. zeaxanthinifaciens, B. algicola, and Z. atlantica) did not exhibit any antibacterial activity against the test bacteria.
The results of this study indicate that colony picking and colony scraping from bacterial cultures on solid agar are more effective methods of primary screening for antibacterial compound-producing marine bacteria.
참고문헌 (42)
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